LOCUS       sequence-211973-        5073 bp    DNA     circular SYN 14-APR-2025
DEFINITION  ProB driven mCherry expression with synTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Cooper KG, Chong A, Starr T, Finn CE, Steele-Mortimer O
  TITLE     Predictable, Tunable Protein Production in Salmonella for Studying 
            Host-Pathogen Interactions.
  JOURNAL   Front Cell Infect Microbiol. 2017 Nov 16;7:475. doi: 
            10.3389/fcimb.2017.00475. eCollection 2017.
  PUBMED    29201859
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol."; date: "2017-11-16"; volume: "7:475. doi"; pages:
            " 10.3389/fcimb.2017.00475. eCollection 2017"
COMMENT     Sequence Label: pCON1-ProB.mCherry
FEATURES             Location/Qualifiers
     source          1..5073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             217..927
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(362..380)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     630..649
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      948..1019
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1035..1062
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     oriT            1675..1762
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     primer_bind     complement(3075..3092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3246..3834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3326..3345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4005..4796,4797..4865))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4628..4647
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4866..4970)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5038..5056
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcgagctcg gtacccgggc acagctaaca ccacgtcgtc cctatctgct gccctaggtc
       61 tatgagtggt tgctggataa ctttacgggc atgcataagg ctcgtaatat atattcaggg
      121 agaccacaac ggtttccctc tacaaataat tttgtttaac ttttactaga gtcacacagg
      181 aaagtactag tctagattta agaaggagat atacatatgg tgagcaaggg cgaggaggat
      241 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
      301 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      361 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      421 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
      481 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      541 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
      601 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
      661 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
      721 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
      781 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
      841 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
      901 ggcggcatgg acgagctgta caagtaagca tgcaagcttc caggcatcaa ataaaacgaa
      961 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1021 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttataaa gcttaatgcg
     1081 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
     1141 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
     1201 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
     1261 gcgtgctgct agcgctttta gccgctttag cggcctttcc ccctacccga agggtggggg
     1321 cgcgtgtgca gccccgcagg gcctgtctcg gtcgatcatt cagcccggct catccttctg
     1381 gcgtggcggc agaccgaaca aggcgcggtc gtggtcgcgt tcaaggtacg catccattgc
     1441 cgccatgagc cgatcctccg gccactcgct gctgttcacc ttggccaaaa tcatggcccc
     1501 caccagcacc ttgcgccttg tttcgttctt gcgctcttgc tgctgttccc ttgcccgcac
     1561 ccgctgaatt tcggcattga ttcgcgctcg ttgttcttcg agcttggcca gccgatccgc
     1621 cgccttgttg ctccccttaa ccatcttgac accccattgt taatgtgctg tctcgtaggc
     1681 tatcatggag gcacagcggc ggcaatcccg accctacttt gtaggggagg gcgcacttac
     1741 cggtttctct tcgagaaact ggcctaacgg ccacccttcg ggcggtgcgc tctccgaggg
     1801 ccattgcatg gagccgaaaa gcaaaagcaa cagcgaggca gcatggcgat ttatcacctt
     1861 acggcgaaaa ccggcagcag gtcgggcggc caatcggcca gggccaaggc cgactacatc
     1921 cagcgcgaag gcaagtatgc ccgcgacatg gatgaagtct tgcacgccga atccgggcac
     1981 atgccggagt tcgtcgagcg gcccgccgac tactgggatg ctgccgacct gtatgaacgc
     2041 gccaatgggc ggctgttcaa ggaggtcgaa tttgccctgc cggtcgagct gaccctcgac
     2101 cagcagaagg cgctggcgtc cgagttcgcc cagcacctga ccggtgccga gcgcctgccg
     2161 tatacgctgg ccatccatgc cggtggcggc gagaacccgc actgccacct gatgatctcc
     2221 gagcggatca atgacggcat cgagcggccc gccgctcagt ggttcaagcg gtacaacggc
     2281 aagaccccgg agaagggcgg ggcacagaag accgaagcgc tcaagcccaa ggcatggctt
     2341 gagcagaccc gcgaggcatg ggccgaccat gccaaccggg cattagagcg ggctggccac
     2401 gacgcccgca ttgaccacag aacacttgag gcgcagggca tcgagcgcct gcccggtgtt
     2461 cacctggggc cgaacgtggt ggagatggaa ggccggggca tccgcaccga ccgggcagac
     2521 gtggccctga acatcgacac cgccaacgcc cagatcatcg acttacagga ataccgggag
     2581 gcaatagacc atgaacgcaa tcgacagagt gaagaaatcc agaggcatca acgagttagc
     2641 ggagcagatc gaaccgctgg cccagagcat ggcgacactg gccgacgaag cccggcaggt
     2701 catgagccag acccagcagg ccagcgaggc gcaggcggcg gagtggctga aagcccagcg
     2761 ccagacaggg gcggcatggg tggagctggc caaagagttg cgggaggtag ccgccgaggt
     2821 gagcagcgcc gcgcagagcg cccggagcgc gtcgcggggg tggcactgga agctatggct
     2881 aaccgtgatg ctggcttcca tgatgcctac ggtggtgctg ctgatcgcat cgttgctctt
     2941 gctcgacctg acgccactga caaccgagga cggctcgatc tggctgcgct tggtggcccg
     3001 atgaagaacg acaggacttt gcaggccata ggccgacagc tcaaggccat gggctgtgag
     3061 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3121 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3181 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3241 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3301 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3421 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3481 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3541 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3601 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3661 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     3721 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3781 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3841 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3901 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3961 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4021 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4081 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4141 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4201 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4261 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4321 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4381 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4441 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4501 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4561 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4621 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4681 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4741 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4801 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4861 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4921 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4981 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     5041 aggcgtatca cgaggccctt tcgtcttcaa gaa
//
