LOCUS       sequence-212600-        7004 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses proteins in a subset of neurons in C. elegans, including 
            isolated expression in the DA9 cholinergic motor neuron in the tail.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Edwards SL, Yorks RM, Morrison LM, Hoover CM, Miller KG
  TITLE     Synapse-Assembly Proteins Maintain Synaptic Vesicle Cluster 
            Stability and Regulate Synaptic Vesicle Transport in Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2015 Sep;201(1):91-116. doi: 10.1534/genetics.115.177337.
  PUBMED    26354975
REFERENCE   2  (bases 1 to 7004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115"; journalName: "Genetics"; date: "2015-09"; 
            volume: "201"; issue: "1"; pages: "91-116"
COMMENT     Sequence Label: KG#471
FEATURES             Location/Qualifiers
     source          1..7004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    120..141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(156..161,162..179,180..186)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    194..210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     199..221
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     218..234
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     218..234
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4803..4825)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4925..4944
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5026..5044)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5112..5216
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5217..5285,5286..6077)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5435..5454)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6248..6836
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6737..6756
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(6990..7004,1..3)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
       61 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      121 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      181 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
      241 tacgccaagc ttgcatgcct gacactaagt ttctctgcag tgttacacga actttttaca
      301 atctactact actaacgaac gtcgcttgta tgtgttgcgc tctgaaaagt tcacatctgt
      361 tgctgccttt tgtacattat ttgagaatct ttactcattt cgtgttcttt tatcaattta
      421 gtgaataaac tgattcatat tgattaacaa gtagttgaac aaaaagaaac ggtgtccttt
      481 tgtgtttgca ttttatctct gtcctttgta gtctgatttt aattttttct tactctattc
      541 atctccttct ctgctccacc aaaccggtat atatgactag ggcttacaca gacgtgcttc
      601 attttctttt cttgcttctt ccccacaaat gatacactgt caatgggcga ggaagaagag
      661 ctgcttcttg ggacggactc gtcgttcttt ctcatcaatc accggcgatg cactcgacac
      721 aaacacgtta caagaaagac cctcccgggc tctttcagcc cattttgttg aaatatgtgc
      781 ttttgaaacg caacattcga atgatttgtc acgacagtcg gcctaattaa ttgactctcg
      841 aaaatgagca tacgattgag tgttaatgtg ccgaagatgt agggaggagc aagaaaagag
      901 ggtggaagag atgatgagtg acagactgtc ttaacagatg acctgcttgt ggtggtcacc
      961 tgtcgagtta tgatctcagt aattgcttca attttccaca tacgttccca agtgtctcat
     1021 tagaatataa ataagacaat attttgttct gtgtcctgta gagaatacac aattccaact
     1081 agaaacgttt tttttcattt ttttaaattg atttcaaaac atcttttgac ctgatgtcag
     1141 tgtgggggat ttttttggca tttgtaatcg aaattaatga atttttagac aatttctgaa
     1201 aaatagtaag gtttaaaaaa atcttaagga cggcacacac tgtacaacaa tactttgact
     1261 gatggttttt ctgtttttca ttacaatctt attttttcta gtcagctttt ttatgtgatt
     1321 atttaaaaaa acaatggtct tgctttagat agtcaagatc tcacactctt aataaaaaag
     1381 gttttaatac ttcagatttt gctttaactt ttggaaattg ccaaccgttt ctgaaaaatt
     1441 tgaagtcttt gaaaacaatt acagcacttt tgcacgtttt gctgctatca aatgtttaat
     1501 ataaataagc aaaatatttg tgaaaaataa aatacttcac ttcatttttt aaattttgct
     1561 tctttctcac aaatgatata ttgactgtaa atgtcagaat tttgaaaagt taaaaaaaat
     1621 tatgttgaaa atttattttt ttttcttact gacagactga tgtgcctttt tcagcatctt
     1681 gaataagttc aagttttttt aatcgaagct gtttccagtg tctgtcagtg acccacacac
     1741 tagaacgcct aaaataaaaa tcgaaatttt tggagtatcc gaatttaatt tggaatcaaa
     1801 ttgatgcttg aaatatatgt gagctcttaa aataataata aaaaattgct acaatcattt
     1861 tattaaaata tttttcgatc taaaaattgt tcatcattga gtaatgtaat tttgataaat
     1921 tagacccccc ccccttcttc tacaaaactg atcaaaaggt ccgaaagact tagaagaccc
     1981 gcacaatagt agaatattca atggccgaat agtgaatgcg cgaggcgggt ggttgctgtc
     2041 ttctcggttc atataaggct catctgttca atcaccaaat gatcgatgtg tttttgtggc
     2101 cttcggcgtc agtttggagc agcacttcaa cctcattgaa acctctgtct tcctcctatc
     2161 tttgcctttt tttagtcaag aggcggtgtg aacgacgcaa tattcccgtt ggttatcacc
     2221 acactagaag tttttttgag cactcttatc agggaacaat tattgatgat ttattgccaa
     2281 ctagtgggtt ggttttgcta ttttttgaaa tttactggta tggtcttttg agattttcag
     2341 gcagttaaaa cttactaaga ctgatggtta acaaaaagtt gtaatataca atactaactg
     2401 aagcgaaact ttgaacgaaa aatatattaa actgttaaat tttatttgca gctagctaac
     2461 taataatttg tatgtttagt gcatttaaaa aagttttcag ctatattata atgatagttt
     2521 gaaactatga taaaactgag tttatacgac tttttgaata cacaaaaaaa atttaaaaat
     2581 tttttgaaaa cttttttaat gtattttttg tagtatttta ttttccctgg cctaggaatt
     2641 atgaaaaaaa gtttctagat ttcaatttat tccttatttt gacaaattat tatgaaataa
     2701 ttcattaatt tcattttgta cgtaaatggg cgggactaat gagatcctca aactccttgt
     2761 gattctcttt cctcctcgcc ttcttctacc atactcctgg acctcgctct tcccgcgcac
     2821 tctcttcccc ctctttcact cttgccacac ttgccaaggg agtcataaat cgtgcaacat
     2881 gtttagtagg tgccgttttg aaatatagat gctctttttt tctttctcac aaaccatctt
     2941 ttagttggtc tcgctcatct attttgaaaa atgacgatcc gtgtagagct ttgattcttc
     3001 atagttattt tcttgtgtgt gcgtcgggcc tcatactgcc aggtagagtg tacgtgttta
     3061 cctaacaagg gagaggtcta tgtggcactg cgcacttcat cgtatgcaaa tttgctttta
     3121 tgatctcttc cctcaggtca ttgtccatca taagcatccg cacacttttt ttttggtgtt
     3181 ttcttctctt tttttgggtc accagttcac aaccaaaaat ataggtggtc aaaacataac
     3241 atttttctgt acttacagta agtggaaatg tcgtctaagc acacttagaa tattctaaat
     3301 aaaagagaat tattagaaaa caactttaaa atgtttctgg caacgttttg aaagtttgag
     3361 aaatttataa caaacgttca aatcaagttg caacataatt agaatataaa atttgtgaaa
     3421 aacacttttt ggtcaacttc caaaattatc aatgatgaaa aagtgactgt ttgcaactat
     3481 ttggctttaa attaaagaac taatttttta ttttaatttt cactatttcc ctacaagcaa
     3541 cctgtaaagt gatattgtgt gtgactattt tttctctttt accacaatat ttgagtatta
     3601 accccccttt agctgtcttc aattatcatt taattcagaa tttcaggtag gcgcgcctct
     3661 agaggatccc cgggattggc caaaggaccc aaaggtatgt ttcgaatgat actaacataa
     3721 catagaacat tttcaggagg acccttggct agcagctctc ctgcagggtt cgacggtacc
     3781 ggtcagatat cactcgagga gctcccgaga tcttcagcgc tcagcattcg tagaattgta
     3841 agtttaaact ttctcatact aactaacgcg ctctatttaa attttcagaa ttccaactga
     3901 gcgccggtcg ctaccattac caacttgtct ggtgtcaaaa ataatagggg ccgctgtcat
     3961 cagagtaagt ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagcatct
     4021 cgcgcccgtg cctctgactt ctaagtccaa ttactcttca acatccctac atgctctttc
     4081 tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt
     4141 tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt
     4201 aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat
     4261 cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt
     4321 tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt
     4381 tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc
     4441 atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat
     4501 taattttcct gcttttgctt tttggggttt cccctattgt ttgtcaagat ttcgaggacg
     4561 gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa
     4621 ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag
     4681 tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca aacataactg
     4741 tttcctacta gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     4801 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     4861 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     4921 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     4981 agatgcgtaa ggagaaaata ccgcatcagg cggccttaat taagggcctc gtgatacgcc
     5041 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5101 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5161 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5221 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5281 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5341 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5401 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5461 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5521 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5581 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5641 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5701 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5761 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5821 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5881 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5941 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6001 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6061 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6121 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6181 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6241 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6301 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6361 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6421 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6481 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6541 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6601 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6661 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6721 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6781 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6841 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6901 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6961 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagc
//
