LOCUS       sequence-212613-       11316 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses wild-type Rpb1 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11316)
  AUTHORS   Morrill SA, Exner AE, Babokhov M, Reinfeld BI, Fuchs SM
  TITLE     DNA Instability Maintains the Repeat Length of the Yeast RNA 
            Polymerase II C-terminal Domain.
  JOURNAL   J Biol Chem. 2016 May 27;291(22):11540-50. doi: 
            10.1074/jbc.M115.696252. Epub 2016 Mar 29.
  PUBMED    27026700
REFERENCE   2  (bases 1 to 11316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.696252"; journalName: "J Biol Chem"; date: 
            "2016-05-27- 27"; volume: "291"; issue: "22"; pages: "11540-50"
COMMENT     Sequence Label: pSMF2
FEATURES             Location/Qualifiers
     source          1..11316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        610..714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(715..783,784..1575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(933..952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1746..2334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2235..2254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2488..2505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2658..2663,2664..2681,2682..2688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2696..2712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2701..2723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2720..2736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2720..2736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9152..9559
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             9560..10654
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(11147..11166)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11266..11288
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgtctcg
     2761 aggagcgtgc agcaccattc accgccagct ttgtatcgtc ctttagagtg cagcaagaac
     2821 attaattgct ctcacagagg tatcataaga acatccgatt cgttcattaa ctgaaatcat
     2881 atctgtgttg ttctgatcga ctctctttgt tagttttatc ttccattttt ttttattcct
     2941 ttcattttcc aataaaattt gaacaggatc gatataaaca gataaaaacc ctccaaagtt
     3001 aaccatttgt ttatatttgc ttttctgcat aagttttcct agcgaattcc ctgatcaact
     3061 ttcaaggaaa actaaaacta ctgtattata agaggttttt tttttcattc cagattaatt
     3121 ttgaaatacg atatcctcaa gtttatctac cagaatattt gactaagaaa tcaaactctg
     3181 ttaataataa tataattata aaaacctcaa ctagaaactc caaaaaaaaa aatttaccat
     3241 tttttacttt ctatccttgt taaccaaatt tcaaaaaaat tttacctttt ctttttccag
     3301 aagagggacc aatcataaag atagcaataa cactttaccc caaaatataa atcagacatg
     3361 gtaggacaac agtattctag tgctccactc cgtacagtaa aagaggtcca attcggtctt
     3421 ttctcacctg aagaagttag agcaatcagt gtggccaaaa ttagatttcc agagacaatg
     3481 gatgaaaccc agacgagagc gaaaattggt ggtctaaacg accctaggtt aggctctatt
     3541 gatcgtaatc tgaagtgtca aacttgtcaa gagggtatga acgaatgtcc tggtcatttt
     3601 ggtcacatag atttagcaaa acctgtattt catgttggtt ttattgccaa aattaagaaa
     3661 gtatgtgagt gtgtctgtat gcactgtggt aagctattac tggatgaaca taatgaatta
     3721 atgagacaag ctctagcaat caaagacagt aaaaaaaggt ttgctgcaat ttggacttta
     3781 tgtaaaacaa aaatggtctg cgaaacagat gtcccttctg aagatgatcc tactcagctc
     3841 gtatcaaggg gaggttgtgg taatacacag cctacaattc gtaaggatgg gttgaaatta
     3901 gttggtagtt ggaaaaaaga tagagccacg ggggatgcgg atgaaccaga actaagagtt
     3961 ttaagtacgg aggaaatctt gaatattttt aagcatatct cagtaaaaga cttcactagt
     4021 ttgggtttca acgaagtttt ttctcgtcca gaatggatga ttttaacatg ccttcctgtc
     4081 ccaccaccac cggtgcgtcc atccatttcc ttcaatgaat ctcaaagagg tgaggatgat
     4141 ttaaccttta aacttgctga tattttaaaa gctaatatta gtttggaaac actagagcat
     4201 aacggtgctc cacatcatgc tattgaagaa gcagagagtt tattacaatt tcatgttgcc
     4261 acttatatgg ataatgatat tgctggtcaa ccacaagctc ttcaaaagtc cggccgtccc
     4321 gttaaatcta ttcgtgctcg tttgaagggt aaagagggtc gtatcagagg taatttaatg
     4381 ggtaagcgtg tggatttttc ggcaagaact gttatttctg gtgatcctaa tttggaatta
     4441 gaccaagtcg gtgttccaaa atctattgcc aagactttaa catacccaga agtggtcaca
     4501 ccatataaca tagatcgtct gacgcaactt gttaggaatg gaccaaatga gcaccccggt
     4561 gccaaatacg tcattcgtga tagcggagac cgtatagatt taagatacag taaaagggca
     4621 ggtgatattc aattacagta tgggtggaaa gttgaacgtc atattatgga caatgatcca
     4681 gttttattca accgtcaacc ttcgttgcac aaaatgtcca tgatggccca cagagtaaaa
     4741 gttattccat attctacatt tagattgaat ttgtccgtta catctccata caatgccgat
     4801 ttcgacggtg acgaaatgaa tcttcacgtt cctcagtctg aggaaacaag ggcggaactt
     4861 tctcaattat gtgctgttcc tctgcaaatt gtttcaccac aatctaacaa accttgtatg
     4921 ggtattgttc aagatacttt gtgtggtatt cgtaaactga cattaagaga tacatttata
     4981 gaacttgatc aagttttgaa tatgctttat tgggttccag attgggatgg tgttattccg
     5041 acacctgcaa ttatcaagcc caaacctttg tggtccggta aacaaatctt gtctgtggct
     5101 atcccaaacg gtattcattt acaacgtttt gatgagggca ctactctgct ttctccaaag
     5161 gataatggta tgcttattat tgacggtcaa atcatttttg gtgtagtaga gaaaaaaacc
     5221 gttggttcct ccaatggtgg tttaattcat gttgttacga gagaaaaggg acctcaagtt
     5281 tgtgctaagt tgtttggtaa catacagaaa gttgttaact tttggttact acataatggg
     5341 ttttcaacag gtattggtga taccattgcg gacggcccaa caatgaggga aattacagag
     5401 acaattgcag aggctaaaaa gaaagttttg gatgttacga aagaagccca ggcaaactta
     5461 ttgactgcta aacatggtat gactctccgt gagtcttttg aggataacgt tgttcggttc
     5521 ctaaatgaag caagagataa ggcaggtcgt ttagctgaag tcaatttgaa agatttgaac
     5581 aatgtgaaac aaatggttat ggcaggttcc aagggttcat ttattaatat cgcgcaaatg
     5641 tcagcttgtg taggacagca atctgttgaa ggtaaacgta ttgcttttgg gttcgttgat
     5701 cgtaccttac ctcatttctc taaagatgat tactccccag agtctaaagg ttttgttgag
     5761 aactcatatt tgagaggttt gaccccacaa gaattttttt tccatgcaat gggtggtcgt
     5821 gaaggtctta tcgataccgc cgtcaaaaca gccgaaacag gttatattca acgtcgttta
     5881 gtgaaagctc tagaagatat catggttcat tacgataaca ccacaagaaa ctcattgggt
     5941 aacgttattc agtttattta tggtgaagat ggtatggatg ctgcgcatat tgaaaagcaa
     6001 tcgctagata ctattggtgg ctccgatgca gcttttgaaa agagatacag agttgattta
     6061 ttgaatacag accataccct tgatccctca ctattggaat ccggatctga gatacttggc
     6121 gatttgaaac ttcaagttct cctggatgaa gaatacaaac aattagtgaa agatcgtaaa
     6181 tttttgaggg aagtttttgt tgatggtgaa gcaaactggc cattaccagt caacataaga
     6241 cgtattattc aaaatgctca acaaactttc cacatagatc atacgaaacc atctgattta
     6301 acaatcaaag acatcgttct tggtgtaaag gatttgcaag aaaacttatt agtgttgcgt
     6361 ggtaagaatg aaattataca aaatgcccag cgagatgcag ttacattgtt ctgctgttta
     6421 ttacgttccc gtttggccac acgtagagtt ctacaagagt acagactaac aaaacaggca
     6481 ttcgattggg tattaagtaa tatcgaggca caattcctcc gttctgttgt tcaccctggt
     6541 gaaatggttg gtgttctagc agcccaatcc attggtgaac cagccacaca aatgaccctt
     6601 aacaccttcc attttgctgg tgttgcttcc aaaaaagtta cttctggtgt cccccgttta
     6661 aaggaaattt tgaatgtggc caaaaacatg aaaacccctt ccttgactgt atacttagag
     6721 cctggtcatg ctgccgatca agaacaagcg aagttgatca gatctgctat cgagcatacc
     6781 actttaaaga gtgtcactat tgcttcagaa atttactatg atcctgatcc acgttccaca
     6841 gttattccag aagatgaaga aattatccaa cttcatttct cattattgga tgaagaggct
     6901 gaacaatctt ttgaccaaca atcaccttgg ttattacgtc tggaactgga tcgtgcagca
     6961 atgaatgata aagacttaac aatgggtcag gttggtgaaa gaatcaagca aacattcaaa
     7021 aatgatttgt ttgttatctg gtctgaagac aacgatgaga agttgatcat ccgttgtcgt
     7081 gttgttcgtc caaagtcact agatgctgag actgaagcag aagaagatca tatgttgaag
     7141 aaaattgaga acacaatgtt agagaatatt acattacgtg gtgtagagaa catcgagcgt
     7201 gttgtcatga tgaaatatga ccgtaaagta ccaagtccaa ctggtgaata cgttaaggaa
     7261 cctgaatggg tgttggaaac agatggtgtt aacttatctg aagttatgac tgttcctggt
     7321 atcgacccaa ccagaatcta taccaactcc ttcattgata taatggaagt tctaggtatt
     7381 gaagctggtc gtgcagcctt gtataaagaa gtttacaatg ttattgcttc tgatggttcg
     7441 tatgttaact accgtcatat ggctttgtta gtcgatgtta tgacaaccca aggtggctta
     7501 acttctgtta ctcgtcatgg tttcaacaga tcaaatacag gtgccttaat gagatgttca
     7561 tttgaagaaa ctgtcgaaat tttgtttgaa gctggtgctt cagccgaatt agatgattgt
     7621 cgtggtgttt cggaaaatgt cattcttggt caaatggctc caatcggtac cggtgcattt
     7681 gatgtgatga tcgatgagga gtcactggta aaatacatgc cagaacaaaa aataactgag
     7741 attgaagacg gacaagatgg tggcgtcaca ccatacagta acgaaagtgg tttggtcaat
     7801 gcagatcttg acgttaaaga tgagctaatg ttttcacctc tggttgattc gggttcaaat
     7861 gacgctatgg ctggaggatt tacagcgtac ggtggtgctg attatggtga agccacgtct
     7921 ccatttggtg cttatggtga agcacctaca tctcccgggt ttggagtctc ctcaccaggc
     7981 ttttctccaa cttccccaac atactctcct acctctccag cgtactcacc aacatcacca
     8041 tcgtactcac caacatcacc atcgtactcg ccaacatcac catcgtactc acctacatca
     8101 ccatcgtatt caccaacgtc accatcatat tcgccaacgt caccatcata ttcgccaacg
     8161 tcgccatcgt attctccaac gtcaccatcg tattcgccaa cgtcgccttc ctactctccc
     8221 acgtcgccaa gctacagccc tacgtctcct tcttattctc ctacatctcc atcatactct
     8281 cctacgtcac caagttacag cccaacgtca ccaagttaca gcccaacgtc tccagcctat
     8341 tccccaacat caccaagtta tagtcctaca tcgccttcat actctccaac atcaccatcc
     8401 tattccccaa catcaccttc ttactctccc acctctccaa actatagccc tacttcacct
     8461 tcttactccc caacatctcc aggctacagc ccaggatctc ctgcatattc tccaaagcaa
     8521 gacgaacaaa agcataatga aaatgaaaat tccagatgag ctcgtatatc atccttacgt
     8581 atttgacgtt attacattat atatagtttc tcaaataata tttctagttt atttttgtat
     8641 cataataaaa acgtatacca aatataccat tatttttcat agcattatgg tagggatagg
     8701 gaatcaagta actaatttat atccgcagag cattgggaaa accaacggcg ctagtaaatg
     8761 catttaaatt acgtccgtcc aacttctaag cttcaagtgg tagactctta actctgaacc
     8821 tttttagcaa ttaagctctt gaagatatca aaagtgttac cgtccggctg taaattataa
     8881 acgtttcctg taaattgagt ggaactaccg cttaccaatt cttttgcaat cagtaaaccg
     8941 tagtcttccg tgataccagt aatcatggct tgcgtatttc cgtgatctgg taatgttact
     9001 atttggttac tatgtaacca caactcataa taacttggca atatttccgc agctccgtag
     9061 ttaataaact gttttaatat gacctcaagg ttattcatat agagtgcctg cgggcccgat
     9121 ccgatatcca ggcctcagct taggcctaga caactgtggg aatactcagg tatcgtaaga
     9181 tgcaagagtt cgaatctctt agcaaccatt atttttttcc tcaacataac gagaacacac
     9241 aggggcgcta tcgcacagaa tcaaattcga tgactggaaa ttttttgtta atttcagagg
     9301 tcgcctgacg catatacctt tttcaactga aaaattggga gaaaaaggaa aggtgagagc
     9361 cgcggaaccg gcttttcata tagaatagag aagcgttcat gactaaatgc ttgcatcaca
     9421 atacttgaag ttgacaatat tatttaagga cctattgttt tttccaatag gtggttagca
     9481 atcgtcttac tttctaactt ttcttacctt ttacatttca gcaatatata tatatatatt
     9541 tcaaggatat accattctaa tgtctgcccc taagaagatc gtcgttttgc caggtgacca
     9601 cgttggtcaa gaaatcacag ccgaagccat taaggttctt aaagctattt ctgatgttcg
     9661 ttccaatgtc aagttcgatt tcgaaaatca tttaattggt ggtgctgcta tcgatgctac
     9721 aggtgtccca cttccagatg aggcgctgga agcctccaag aaggttgatg ccgttttgtt
     9781 aggtgctgtg ggtggtccta aatggggtac aggtagtgtt agacctgaac aaggtttact
     9841 aaaaatccgt aaagaacttc aattgtacgc caacttaaga ccatgtaact ttgcatccga
     9901 ctctctttta gacttatctc caatcaagcc acaatttgct aaaggtactg acttcgttgt
     9961 tgtcagagaa ttagtgggag gtatttactt tggtaagaga aaggaagacg atggtgatgg
    10021 tgtcgcttgg gatagtgaac aatacaccgt tccagaagtg caaagaatca caagaatggc
    10081 cgctttcatg gccctacaac atgagccacc attgcctatt tggtccttgg ataaagctaa
    10141 tgttttggcc tcttcaagat tatggagaaa aactgtggag gaaaccatca agaacgaatt
    10201 tcctacattg aaggttcaac atcaattgat tgattctgcc gccatgatcc tagttaagaa
    10261 cccaacccac ctaaatggta ttataatcac cagcaacatg tttggtgata tcatctccga
    10321 tgaagcctcc gttatcccag gttccttggg tttgttgcca tctgcgtcct tggcctcttt
    10381 gccagacaag aacaccgcat ttggtttgta cgaaccatgc cacggttctg ctccagattt
    10441 gccaaagaat aaggttgacc ctatcgccac tatcttgtct gctgcaatga tgttgaaatt
    10501 gtcattgaac ttgcctgaag aaggtaaggc cattgaagat gcagttaaaa aggttttgga
    10561 tgcaggtatc agaactggtg atttaggtgg ttccaacagt accaccgaag tcggtgatgc
    10621 tgtcgccgaa gaagttaaga aaatccttgc ttaaaaagat tctctttttt tatgatattt
    10681 gtacataaac tttataaatg aaattcataa tagaaacgac acgaaattac aaaatggaat
    10741 atgttcatag ggtagacgaa actatatacg caatctacat acatttatca agaaggagaa
    10801 aaaggaggat agtaaaggaa tacaggtaag caaattgata ctaatggctc aacgtgataa
    10861 ggaaaaagaa ttgcacttta acattaatat tgacaaggag gagggcacca cacaaaaagt
    10921 taggtgtaac agaaaatcat gaaactacga ttcctaattt gatattggag gattttctct
    10981 aaaaaaaaaa aaatacaaca aataaaaaac actcaatgac ctgaccattt gatggagttt
    11041 aagtcaatac cttcttgaac catttcccat aatggtgaaa gttccctcaa gaattttact
    11101 ctgtcagaaa cggccttacg acgtagtcga tatggtgcac tctcagtaca atctgctctg
    11161 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
    11221 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
    11281 gtcagaggtt ttcaccgtca tcaccgaaac gcgcga
//
