LOCUS       sequence-212637-        2407 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  For tetracycline-inducible expression of bio-myc-NES-mCE Delta-NLS 
            (""bio-myc-cCE"") in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Trotman JB, Agana BA, Giltmier AJ, Wysocki VH, Schoenberg DR
  TITLE     RNA-binding proteins and heat-shock protein 90 are constituents of 
            the cytoplasmic capping enzyme interactome.
  JOURNAL   J Biol Chem. 2018 Oct 26;293(43):16596-16607. doi: 
            10.1074/jbc.RA118.004973. Epub 2018 Aug 30.
  PUBMED    30166341
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.004973"; journalName: "J Biol Chem"; date: 
            "2018-10-26- 26"; volume: "293"; issue: "43"; pages: "16596-16607"
COMMENT     Sequence Label: pcDNA4/TO-bio-myc-cCE
FEATURES             Location/Qualifiers
     source          1..2407
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             427..453
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             2249..2272
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2301..2318)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctccggact
       61 ctagcgttta aacttaagct tggtaccatg gagttaaaaa ccgccgcact agctcaacat
      121 gacgaggctg ctggaaaggc cggtgaaggt gaaatccctg cccctcttgc tggtaccgtt
      181 tctaagatac tggtaaaaga aggtgacact gttaaagctg gtcaaacagt tctggtgctg
      241 gaggctatga aaatggagac agaaattaac gctcctactg acggaaaagt tgaaaaggtg
      301 ttagttaagg aaagagatgc tgttcaaggt ggtcaaggtc taatcaagat cggcgttggc
      361 accatggcat caatgcagaa gctgatctca gaggaggacc tgcttatggc catggaggcc
      421 cttcagctac caccgcttga gagacttact cttgatatgg ccatggaggc cgcttacaac
      481 aagatcccgc cgcggtggct gaactgtccg cggcgcggtc agccggtggc aggaagattc
      541 ttacctctga agacgatgct ggggccaaga tatgatagtc aagttgctga agaaaaccgg
      601 ttccatccca gtatgctctc aaattatcta aagagcctga aggttaaaat gagcttgttg
      661 gtggatctga caaatacttc aaggttctat gacagaaatg acatagaaaa agaaggaatc
      721 aagtatatca aacttcagtg taaaggacat ggcgaatgcc ccaccactga gaatactgag
      781 acattcattc gcctgtgtga gaggtttaat gaaagaagcc cacctgaact tataggtgtt
      841 cactgcaccc atggcttcaa ccgcactggt ttccttatat gtgccttttt ggtggaaaaa
      901 atggattgga gtattgaagc agcagttgct acatttgccc aagccagacc accaggaatc
      961 tacaagggtg attatttgaa ggaacttttt cgtcgctatg gagatataga agaagcacca
     1021 ccaccacctg tattgccaga ttggtgtttt gaagatgagg atgaagaaga tgaggatgaa
     1081 gatggaaaaa aggactcaga accagggtca agtgcatcct ttagtaaaag gagaaaagag
     1141 cggttaaaac tgggagctat tttcttggaa ggcataactg ttaaaggtgt aactcaagta
     1201 acaactcaac ccaagttagg agaggtgcag cagaaatgtc atcagttctg tggctgggaa
     1261 gggtctgggt tccctggagc acagcctgtc tccatggaca agcaaaatat tagactttta
     1321 gagcagaagc cttataaagt aagctggaaa gcagacggca ctcgttacat gatgttgatt
     1381 gatggcacaa atgaagtttt tatgattgac agagataatt ccgtttttca tgtttcaaat
     1441 ctggaatttc catttcgtaa agatcttcgc atgcatttat caaatactct tttggatggg
     1501 gaaatgatca ttgacaaagt aaatggacag gccgtcccaa gatatttgat ctatgacata
     1561 attaagttca atgcacaacc agttggagac tgtgatttta atattcgtct acagtgtatt
     1621 gaacgtgaaa ttataagtcc acgacatgaa aaaatgaaga ctggactcat tgacaaaaca
     1681 caggaaccat ttagtgtcag acctaaacaa ttttttgaca tcaatatttc aagaaagctt
     1741 ctggaaggaa attttgccaa agaagtcagc catgaaatgg atggacttat ttttcagcct
     1801 attggaaaat acaagcctgg tcgatgtgat gacattttga aatggaaacc tcccagtcta
     1861 aactctgtgg attttcgact taagataaca agaatgggag gagaagggtt gcttccacag
     1921 aatgttggcc ttctctatgt tggaggttat gaaagaccct ttgcacaaat caaggtgaca
     1981 aaagaactaa aacagtatga caacaaaatt atagaatgca aatttgagaa caatagctgg
     2041 gtcttcatga gacagaggat agacaaaagt ttcccaaatg cctacaacac agccatggct
     2101 gtgtgcaata gcatctcgaa ccctgtcacc aaggagatgc tgtttgaatt cattgacaga
     2161 tgtgcagcag ccgcccaggg acagcccctg gaccctgaca cggagctcat gccgccccca
     2221 ccgcccaaaa gactgcatcg gccaacctga ttacaaggat gacgacgata agtaggggcc
     2281 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     2341 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     2401 aaatgag
//
