LOCUS       sequence-21264-e         781 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Fiordalisi JJ, Johnson RL, Ulku AS, Der CJ, Cox AD
  TITLE     Mammalian expression vectors for Ras family proteins: generation and
            use of expression constructs to analyze Ras family function.
  JOURNAL   Methods Enzymol. 2001  . 332():3-36.
  PUBMED    11305105
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol. 2001  . 332():3-36."
COMMENT     Sequence Label: pCGN R-Ras 43N
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           109..148
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             178..204
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             184..210
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 cgtggagnat tagcagagct cgttagtgaa ccgtcagatc gcctggagac gccatccacg
       61 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggggga tcttggtggc
      121 gtgaaactcc cgcacctctt cggccagcgc cttgtagaag cgcgtatggc ttctagctat
      181 ccttatgacg tgcctgacta tgccagcctg ggaggacctt ctagcggatc cgtcgacatg
      241 agctctggtg ctgcttccgg tacaggtcgt ggtcgtccac gtggtggagg tccaggtccc
      301 ggggaccctc cgcctagcga gacacacaag cttgtggtcg tgggtggtgg tggcgtgggc
      361 aagaacgcgc tgaccattca gttcatccag tcctacttcg tgtctgacta cgaccccact
      421 attgaggact cctacacgaa gatctgcagt gtggatggca tcccagcccg gctggacatc
      481 ctggacaccg cgggccagga agagttcggg gccatgagag agcagtacat gcgtgctggc
      541 cacggcttcc tgctggtgtt cgccattaat gaccggcaga gtttcaacga ggtgggcaag
      601 ctcttcacgc agattctgcg ggtcaaggac cgcgacgact tccccgttgt gttggtcggg
      661 aacaaggcag atctggagtc acagcgccag gtcccccgat cagaagcctc tgccttcggc
      721 gcctcccacc acgtggccta ctttgangcc tcggccaaac tgcgtctcac tgaagtatgn
      781 c
//
