LOCUS       sequence-212687-        9964 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses a plasma membrane calcium pump hPMCA2w/b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Yu X, Taylor AMW, Nagai J, Golshani P, Evans CJ, Coppola G, Khakh BS
  TITLE     Reducing Astrocyte Calcium Signaling In Vivo Alters Striatal 
            Microcircuits and Causes Repetitive Behavior.
  JOURNAL   Neuron. 2018 Aug 27. pii: S0896-6273(18)30688-3. doi: 
            10.1016/j.neuron.2018.08.015.
  PUBMED    30174118
REFERENCE   2  (bases 1 to 9964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2018 Aug 27. pii: S0896-6273(18)30688-3. doi: 
            10.1016/j.neuron.2018.08.015."
COMMENT     Sequence Label: pcDNA3.1-CMV-mCherry-hPMCA2w/b
FEATURES             Location/Qualifiers
     source          1..9964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        691..709
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             715..1422
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(860..878)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1128..1147
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1438..1464
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1465..1482
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1483..1500
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      1552..1561
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2059..2070)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      2620..2629
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5321..5338)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5327..5551
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5597..6025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5684..5703)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5894..5915
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6034..6054)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6039..6368
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6219..6354
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6281..6300
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6435..7229
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6489..6508)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7099..7118
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7403..7524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7440..7459)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7494..7513
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7573..7589)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7573..7589)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7586..7608)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7597..7613
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7621..7627,7628..7645,7646..7651))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7666..7687
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7804..7821)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7975..8563)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8055..8074)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8734..9525,9526..9594))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9357..9376
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9595..9699)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9774..9793)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagg ctagagtact taatacgact cactataggc tagcatggtg
      721 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
      781 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      841 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
      901 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
      961 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     1021 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     1081 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     1141 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     1201 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     1261 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     1321 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     1381 gagggccgcc actccaccgg cggcatggac gagctgtaca agtccggact cagatcctac
     1441 ccctatgatg tgcctgacta tgcccaccac caccaccacc acctggtgcc caggggcagc
     1501 agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcggtggc ggccgccatg
     1561 ggtgacatga ccaacagcga cttttactcc aaaaaccaaa gaaatgagtc gagccatggg
     1621 ggcgagttcg ggtgcacaat ggaggagctc cgctccctca tggagctgcg gggcactgag
     1681 gctgtggtca agatcaagga gacttatggg gacaccgaag ccatctgccg gcgcctcaaa
     1741 acctcacctg ttgaaggttt gccggacacc gctccagacc tggaaaagag aaagcaaatt
     1801 tttgggcaaa actttatacc tccaaagaag ccaaaaacct tcctgcagct cgtgtgggag
     1861 gcgctgcagg acgtgacgct catcatcctg gagattgccg ccatcatctc cctggggctg
     1921 tccttctacc acccgcccgg cgagggcaac gaaggatgtg cgacggccca gggtggggca
     1981 gaggatgaag gagaggcaga ggcaggttgg atcgaggggg ccgccattct cctctcagtt
     2041 atctgtgtgg tcctggtcac ggccttcaat gactggagca aagagaaaca gttccggggc
     2101 ctgcagagcc gcatcgagca ggaacagaaa tttaccgtag tccgggctgg ccaggtggtg
     2161 cagatccctg tggctgagat cgtggttggg gacatagccc aggtcaaata tggtgacctc
     2221 ctccctgccg acggcctctt catccagggc aatgacctca agattgatga aagctcccta
     2281 actggagagt ctgaccaggt gcgcaagtcc gtggacaagg accccatgct gctgtcagga
     2341 acccacgtga tggagggctc aggacggatg ttggtgactg ctgtgggtgt gaactctcag
     2401 actggcatca tctttaccct cctgggggct ggtggtgaag aggaagagaa gaaagacaaa
     2461 aaaggtgtga agaaggggga tggccttcag ctaccagcag cagacggtgc ggcagcttca
     2521 aatgctgcag atagtgcgaa tgccagccta gtcaatggta aaatgcagga tggcaatgtg
     2581 gacgccagcc agagcaaagc caaacagcag gacggggcag ccgccatgga gatgcagccc
     2641 ctcaagagtg ccgagggcgg cgacgctgac gacaggaaga aggccagcat gcacaagaag
     2701 gagaagtccg tgctgcaggg caagctcacc aagctggctg tgcagatcgg gaaggcgggc
     2761 ttggtgatgt cagccatcac ggtgatcatc ctggtgctct acttcactgt ggacaccttc
     2821 gtggtcaaca agaagccgtg gctgcctgag tgcacgcccg tctacgtgca gtactttgtc
     2881 aagttcttca tcattggcgt gacggtgctg gtggtcgccg tgcccgaggg gctccctctg
     2941 gccgtcacca tctcgttggc ctattcggtg aagaaaatga tgaaggacaa caacctggta
     3001 cgccacctgg atgcctgtga gaccatgggc aatgccacag ccatctgctc agacaagaca
     3061 ggcacgctga ccaccaatcg catgacagtg gtacaggcct atgtcggcga cgtccactat
     3121 aaagagatcc ccgaccccag ctccatcaac accaagacca tggagctgct gatcaatgcc
     3181 atcgccatca acagcgccta caccaccaag attctgcccc cagagaagga gggcgccctg
     3241 cctcggcagg tgggcaacaa gacggagtgc ggcctgctgg gcttcgtgct ggacctgaag
     3301 caggactacg agcccgtgcg cagccagatg ccagaggaga agttgtacaa agtgtacacc
     3361 ttcaactccg tgcgcaagtc catgagcact gtcatcaagc tgcccgacga gagcttccgc
     3421 atgtacagca agggggcttc tgagatcgtg ctcaagaagt gctgcaaaat cctcaatggg
     3481 gcgggagagc ctcgtgtctt ccggccccgc gaccgggacg agatggtaaa gaaggtgatt
     3541 gagcccatgg cttgcgatgg gctccgcact atctgcgtgg cctaccgcga cttccccagc
     3601 agcccggagc cggactggga caatgagaat gacatcctca acgaactcac ctgcatctgc
     3661 gtggtgggca tcgaggaccc ggtgcggcca gaggtcccag aagccatccg caagtgccag
     3721 cgggcaggca tcacggtccg catggtcact ggcgacaata tcaacacggc tcgggccatc
     3781 gccatcaagt gtggcatcat ccatcctggg gaggactttc tgtgcctcga gggcaaggag
     3841 ttcaacagga ggatccgcaa cgagaagggg gagattgagc aggagcgaat tgacaagatc
     3901 tggccaaagc tgcgggtgct ggctcgctcc tccccaacgg acaagcatac cctggttaaa
     3961 ggcatcatcg acagcacaca cactgagcag cggcaggtgg tggccgtgac gggggacggg
     4021 accaacgacg ggcctgcact caagaaggcc gacgtgggct tcgccatggg catcgcaggc
     4081 actgacgtgg ccaaggaggc ctcagacatc atcctgacag acgacaattt cagcagcatc
     4141 gtcaaggcag tgatgtgggg ccgcaacgtc tatgacagca tctccaaatt cttgcagttc
     4201 cagctcaccg tcaacgtggt ggccgtgatt gtggccttca caggcgcctg catcacgcag
     4261 gactcccctc tgaaggccgt gcagatgctc tgggtgaacc tcatcatgga cacgtttgcc
     4321 tcgctggcac tggccactga gccgcccacg gagaccctgc tgctgaggaa gccgtacggc
     4381 cgcaacaagc cgctcatctc caggaccatg atgaagaaca tcctgggcca tgctgtctac
     4441 cagcttgccc tcatcttcac cctgctcttt gttggcgaga agatgttcca gatcgacagc
     4501 gggaggaacg cgcccctgca ttcgccaccc tcagaacatt acaccatcat cttcaacacc
     4561 ttcgtcatga tgcagctctt caacgagatc aacgcccgca agatccacgg cgagcgcaat
     4621 gtctttgacg gcatcttccg gaaccccatc ttctgcacca tcgtgctggg cacctttgcc
     4681 atccagatag tgatcgtgca gtttggaggg aagccattca gctgctctcc actgcagctg
     4741 gaccagtgga tgtggtgcat attcattggg ttaggagagc tcgtttgggg ccaggtcatc
     4801 gccaccatcc cgaccagcag actcaagttc ctcaaggagg caggcaggct cacacagaag
     4861 gaggagatcc cggaggagga gctcaacgag gacgtggagg agatcgacca cgcggagcgg
     4921 gagctgcggc ggggccagat cctgtggttc cgaggcctga atcggatcca gacacagatc
     4981 cgcgtcgtga aggcgttccg tagctctctc tatgaaggtt tagaaaagcc tgaatctcga
     5041 acctccatcc ataacttcat ggctcatcct gaattccgga tcgaagattc ccagccccac
     5101 atccccctca ttgatgacac cgacctggaa gaagatgccg cgctcaagca gaactcgagc
     5161 ccgccgtcat ccctcaacaa gaacaacagc gccatcgaca gtgggatcaa cctgacgacc
     5221 gacacaagca aatcagctac ctcttcaagt ccagggagcc ccatccacag cctggagacg
     5281 tcgctttagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     5341 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5401 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     5461 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     5521 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     5581 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5641 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5701 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5761 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5821 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5881 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5941 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6001 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     6061 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6121 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6181 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6241 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6301 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6361 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     6421 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     6481 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     6541 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     6601 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     6661 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     6721 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     6781 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     6841 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     6901 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     6961 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     7021 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     7081 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     7141 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     7201 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     7261 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     7321 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     7381 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     7441 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7501 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     7561 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7621 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7681 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7741 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7801 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7861 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7921 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7981 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8041 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8101 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8161 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8221 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8281 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8341 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8401 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8461 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8521 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8581 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8641 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8701 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8761 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8821 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8881 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8941 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9001 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9061 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9121 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9181 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9241 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9301 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9361 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9421 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9481 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9541 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9601 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9661 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9721 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     9781 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     9841 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     9901 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     9961 cgtt
//
