LOCUS       sequence-212862-        8304 bp    DNA     circular SYN 14-APR-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8304)
  AUTHORS   Liu X, Salokas K, Tamene F, Jiu Y, Weldatsadik RG, Ohman T, 
            Varjosalo M
  TITLE     An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping
            of protein interactions and subcellular localizations.
  JOURNAL   Nat Commun. 2018 Mar 22;9(1):1188. doi: 10.1038/s41467-018-03523-2.
  PUBMED    29568061
REFERENCE   2  (bases 1 to 8304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-22"; volume: "9(1):1188. doi"; pages: " 
            10.1038/s41467-018-03523-2"
COMMENT     Sequence Label: MAC_N_LMNA
FEATURES             Location/Qualifiers
     source          1..8304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        31..410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        411..614
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    616..634
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    637..655
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     665..689
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(857..1207,1208..1210,1211..1819)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             1820..1846
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1877..1900
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1937..1960
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    1973..1997
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3990..4014)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4052..4069)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4058..4282
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4415..4434)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4566..4613
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4621..5643
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4621..5643
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5773..5894
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5810..5829)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5864..5883
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5943..5959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5943..5959)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5956..5978)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5967..5983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5991..5997,5998..6015,6016..6021))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6036..6057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6174..6191)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6345..6933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6425..6444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7104..7895,7896..7964))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7727..7746
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7965..8069)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8144..8163)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
       61 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      121 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      181 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      241 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      301 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      361 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      421 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      481 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      541 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      601 atataagcag agctctccct atcagtgata gagatctccc tatcagtgat agagatcgtc
      661 gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
      721 tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa cttaagcttg
      781 gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg
      841 gcggccgctc gagaccatga aggacaacac cgtgcccctg aagctgatcg ccctgctggc
      901 caacggcgag ttccactctg gcgagcagct gggcgagaca ctgggcatga gcagagccgc
      961 catcaacaag cacatccaga ccctgcggga ctggggcgtg gacgtgttca cagtgcccgg
     1021 caagggctac agcctgcccg agcccatcca gctgctgaac gccaagcaga tcctgggcca
     1081 gctggatggc ggatctgtgg ccgtgctgcc cgtgatcgac agcaccaacc agtatctgct
     1141 cgaccggatc ggcgagctga agtccggcga cgcctgtatc gccgagtacc agcaggccgg
     1201 cagaggcggc aggggcagaa agtggttcag ccccttcggc gccaacctgt acctgagcat
     1261 gttctggaga ctggaacagg gcccagccgc cgctattggc ctgagcctgg tcatcggcat
     1321 cgtgatggcc gaggtgctgc ggaagctggg cgccgacaaa gtgcgcgtga agtggcccaa
     1381 cgacctgtat ctgcaggacc ggaagctggc cggcatcctg gtggaactga ccggcaaaac
     1441 aggcgacgcc gcccagatcg tgatcggcgc tggcatcaac atggccatgc ggcgggtgga
     1501 agagagcgtg gtcaaccagg gctggatcac cctgcaggaa gccggcatca acctggaccg
     1561 gaacaccctg gccgccatgc tgatcagaga gctgagagcc gccctggaac tgttcgagca
     1621 ggaaggcctg gccccctacc tgagcagatg ggagaagctg gacaacttca tcaaccggcc
     1681 cgtgaaactg atcatcggcg acaaagagat cttcggcatc agccggggca tcgacaagca
     1741 gggggccctg ctcctggaac aggacggcat catcaagccc tggatgggcg gcgagatcag
     1801 cctgcggagc gccgagaagt acccatacga tgttcctgac tatgccggta ccgagctcgg
     1861 atccaccatg gctagctgga gccacccgca gttcgagaaa ggtggaggtt ccggaggtgg
     1921 atcgggaggt ggatcgtgga gccacccgca gttcgaaaaa gcggccgata tcacaagttt
     1981 gtacaaaaaa gttggcatgg agaccccgtc ccagcggcgc gccacccgca gcggggcgca
     2041 ggccagctcc actccgctgt cgcccacccg catcacccgg ctgcaggaga aggaggacct
     2101 gcaggagctc aatgatcgct tggcggtcta catcgaccgt gtgcgctcgc tggaaacgga
     2161 gaacgcaggg ctgcgccttc gcatcaccga gtctgaagag gtggtcagcc gcgaggtgtc
     2221 cggcatcaag gccgcctacg aggccgagct cggggatgcc cgcaagaccc ttgactcagt
     2281 agccaaggag cgcgcccgcc tgcagctgga gctgagcaaa gtgcgtgagg agtttaagga
     2341 gctgaaagcg cgcaatacca agaaggaggg tgacctgata gctgctcagg ctcggctgaa
     2401 ggacctggag gctctgctga actccaagga ggccgcactg agcactgctc tcagtgagaa
     2461 gcgcacgctg gagggcgagc tgcatgatct gcggggccag gtggccaagc ttgaggcagc
     2521 cctaggtgag gccaagaagc aacttcagga tgagatgctg cggcgggtgg atgctgagaa
     2581 caggctgcag accatgaagg aggaactgga cttccagaag aacatctaca gtgaggagct
     2641 gcgtgagacc aagcgccgtc atgagacccg actggtggag attgacaatg ggaagcagcg
     2701 tgagtttgag agccggctgg cggatgcgct gcaggaactg cgggcccagc atgaggacca
     2761 ggtggagcag tataagaagg agctggagaa gacttattct gccaagctgg acaatgccag
     2821 gcagtctgct gagaggaaca gcaacctggt gggggctgcc cacgaggagc tgcagcagtc
     2881 gcgcatccgc atcgacagcc tctctgccca gctcagccag ctccagaagc agctggcagc
     2941 caaggaggcg aagcttcgag acctggagga ctcactggcc cgtgagcggg acaccagccg
     3001 gcggctgctg gcggaaaagg agcgggagat ggccgagatg cgggcaagga tgcagcagca
     3061 gctggacgag taccaggagc ttctggacat caagctggcc ctggacatgg agatccacgc
     3121 ctaccgcaag ctcttggagg gcgaggagga gaggctacgc ctgtccccca gccctacctc
     3181 gcagcgcagc cgtggccgtg cttcctctca ctcatcccag acacagggtg ggggcagcgt
     3241 caccaaaaag cgcaaactgg agtccactga gagccgcagc agcttctcac agcacgcacg
     3301 cactagcggg cgcgtggccg tggaggaggt ggatgaggag ggcaagtttg tccggctgcg
     3361 caacaagtcc aatgaggacc agtccatggg caattggcag atcaagcgcc agaatggaga
     3421 tgatcccttg ctgacttacc ggttcccacc aaagttcacc ctgaaggctg ggcaggtggt
     3481 gacgatctgg gctgcaggag ctggggccac ccacagcccc cctaccgacc tggtgtggaa
     3541 ggcacagaac acctggggct gcgggaacag cctgcgtacg gctctcatca actccactgg
     3601 ggaagaagtg gccatgcgca agctggtgcg ctcagtgact gtggttgagg acgacgagga
     3661 tgaggatgga gatgacctgc tccatcacca ccacggctcc cactgcagca gctcggggga
     3721 ccccgctgag tacaacctgc gctcgcgcac cgtgctgtgc gggacctgcg ggcagcctgc
     3781 cgacaaggca tctgccagcg gctcaggagc ccaggtgggc ggacccatct cctctggctc
     3841 ttctgcctcc agtgtcacgg tcactcgcag ctaccgcagt gtggggggca gtgggggtgg
     3901 cagcttcggg gacaatctgg tcacccgctc ctacctcctg ggcaactcca gcccccgaac
     3961 ccagagcccc cagaactgca gcatcatgta cccaactttc ttgtacaaag tggtgacgta
     4021 agctaggggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     4081 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4141 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4201 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4261 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     4321 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4381 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4441 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4501 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4561 acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttccttggcc
     4621 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     4681 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     4741 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     4801 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     4861 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     4921 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     4981 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     5041 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     5101 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     5161 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     5221 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     5281 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     5341 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     5401 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     5461 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     5521 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     5581 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     5641 tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     5701 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     5761 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5821 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5881 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     5941 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6001 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6061 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6121 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6181 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6241 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6301 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6361 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6421 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6481 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6541 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6601 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6661 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6721 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6781 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6841 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6901 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6961 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7021 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7081 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7141 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7201 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7261 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7321 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7381 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7441 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7501 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7561 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7621 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7681 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7741 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7801 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7861 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7921 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7981 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8041 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     8101 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     8161 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     8221 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     8281 ttagggttag gcgttttgcg ctgc
//
