LOCUS       sequence-213735-        7941 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Truncated Hsh155 (aa 64-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 64-972
FEATURES             Location/Qualifiers
     source          1..7941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3324..3344)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3324..3342)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3363..3379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3363..3379)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3376..3398)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3387..3403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3411..3417,3418..3435,3436..3441))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3456..3477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3594..3611)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3765..4353)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3845..3864)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4524..5315,5316..5384))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5147..5166
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5385..5489)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    5526..6029
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     6070..6088
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6126..6148)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6248..6267
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6288..6568
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6569..7243
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7346..7801)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7483..7504)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7695..7714
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(7927..7941,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(7941,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
       61 ggcccccccc tcgagcatgt actttccgcc taactaccat cacctgtctt tatttattcc
      121 gctgacagat atgcacaacc aattatcgtc tcttgaaagc tttttagacg tgattgctgt
      181 tatttggcac gttgttagta tttaagctta ttcgggtttg cattgatatt tgcccgagca
      241 gaaatctcct tctcgatgag ttagaactga aacatctctt caattactta tacataataa
      301 acatagaaag ccaggacctg tcatcatgta ccacaaaaga aggtttgata tgaagtttga
      361 gccggattcc gatacacaaa ctgttacgtc ttcagaaaat acccaagacg ctgtcgtgcc
      421 aaggaaaaga aaatctcgtt gggatgtaaa agggtacgag ccacctgatg aatcttcaac
      481 agcggtcaaa gaaaactctg atagtgcttt ggttaatgta gaaggaatcc atgatttgat
      541 gttttttaaa ccttctgacc ataaatattt cgccgacgtt atatccaaaa aacccataga
      601 tgagttaaat aaggatgaaa agaaagaaag aaccctgtcg atgttacttc tgaaaataaa
      661 aaatggcaat acagcgagta ggaggacttc tatgcggatt ttaaccgata aagctgttac
      721 atttggtcca gaaatgatat ttaaccgctt attgcccata ttactagaca gaagtttgga
      781 agaccaggaa agacatctaa tgatcaagac catagatcgc gtcctttacc agctaggaga
      841 tttaacaaaa ccttacgttc acaaaatact tgtagtagct gccccattgc taatagatga
      901 agatccaatg gttcgttcaa caggccagga gataatcacc aatctgtcta cagtcgctgg
      961 cctaaaaacc attctaacag taatgagacc tgatattgag aatgaggatg agtatgttag
     1021 aaatgtaact tcaagagcgg cggctgtggt ggcaaaagcc cttggtgtta atcaattgct
     1081 tcctttcatc aatgcagcat gccactcacg gaagtcttgg aaagcaaggc atactggcat
     1141 aaaaattgtg cagcaaatag gtatactttt gggaataggt gtcttgaatc atcttactgg
     1201 gctgatgagt tgtatcaaag actgcctaat ggatgatcac gttccggtgc gaattgttac
     1261 agcacatact ttatcaacac tagcagaaaa ttcttatcca tatggtatcg aagttttcaa
     1321 cgttgtactg gagccattat ggaagggtat aagaagtcat cgtggtaaag tgctatcttc
     1381 ttttttaaaa gctgttggat ctatgatccc attgatggac ccagagtatg ccggttatta
     1441 taccacagaa gcaatgagaa taattagacg tgaattcgac tctcccgatg atgaaatgaa
     1501 aaagaccatt cttttagtat tacaaaaatg tagcgctgta gaatccataa cgccaaaatt
     1561 tctgagagaa gaaatcgcgc cagaattctt tcaaaaattt tgggtcagac gagtggcatt
     1621 ggatagacca ttaaacaaag ttgttacata tactactgtt acattagcaa agaaattagg
     1681 ttgttcttat accattgata aattattaac accattgaga gatgaagctg agccgttcag
     1741 aacaatggct gtccatgctg ttactagaac agtaaactta ttagggacag cagatttaga
     1801 tgagagatta gaaacgaggc ttattgatgc gctcttgata gcatttcaag aacaaacaaa
     1861 tagtgattct ataatcttta aagggtttgg ggcagtgacg gtgtcacttg acatccgtat
     1921 gaaaccgttt ttagcaccca ttgtgagtac gattttaaat catttaaagc ataaaactcc
     1981 cttggttcgt caacatgcag cagatctatg tgccatacta ataccagtca tcaagaactg
     2041 tcacgaattc gaaatgttga ataaattgaa cattatattg tatgaatcct taggcgaagt
     2101 atatccagag gtattaggtt ctataatcaa cgctatgtac tgtattacta gcgtaatgga
     2161 tttggataaa ctacaacctc ctattaatca aattttacca accttaactc caattttaag
     2221 aaacaagcat agaaaagtcg aagtaaacac tatcaaattt gttggactta ttggtaaatt
     2281 agcacctacc tatgcacctc ctaaagaatg gatgagaata tgttttgaac tattagaact
     2341 tttgaaaagt acaaataaag aaataagaag atcagcaaat gccacgtttg gattcattgc
     2401 tgaagcaatt ggacctcacg atgttcttgt tgcgttattg aacaatttaa aggttcaaga
     2461 acgtcagtta cgtgtatgca cggctgttgc tatcggtatc gttgctaaag tgtgtggtcc
     2521 ttacaacgtg ctgccagtaa tcatgaatga atacacaaca ccagagacta atgttcaaaa
     2581 cggtgttctc aaggccatgt cattcatgtt tgagtacatt ggtaacatgt ctaaggatta
     2641 catatacttc ataacaccat tattagaaga tgcactcacc gatagggatt tagttcatcg
     2701 tcagacagcg tcgaacgtaa taacccactt agctttaaac tgttcaggta caggtcacga
     2761 agatgccttc attcatttaa tgaatcttct aatacccaat atttttgaaa cttcaccaca
     2821 tgctattatg cgtattttag aaggtttgga ggcactgagt caagctcttg gccctggact
     2881 attcatgaac tacatatggg caggattgtt ccatccagcg aagaatgtaa ggaaagcatt
     2941 ttggagggtg tataataaca tgtacgtaat gtaccaggac gccatggtac ctttttaccc
     3001 cgttacacca gacaacaatg aagaatatat agaagaactg gatttagttc tgtgaatata
     3061 tatagagcat ataaataacc acaacttgta agaatatttt acttgacaca cctatatcat
     3121 cacgtcacga tgccttcata gattatgatg aaatataaac agaacctgct attggaaaaa
     3181 aaagggcagg aaaaaatttt gtgaaagtga gtaaaagaaa gatgaagagc gagacatgga
     3241 taataagaaa tagatgtcac taatggacaa ttggaagact gatatggaaa gttacgatga
     3301 aggaggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     3361 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     3421 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     3481 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     3541 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     3601 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3661 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3721 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3781 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3841 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3901 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3961 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4021 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4081 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4141 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4201 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4261 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4321 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4381 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4441 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4501 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4561 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4621 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4681 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4741 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4801 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4861 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4921 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4981 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5041 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5101 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5161 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5221 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5281 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5341 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5401 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5461 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc
     5521 ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa atatataaat
     5581 taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt
     5641 aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca
     5701 ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt
     5761 gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc
     5821 taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt
     5881 tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa
     5941 aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg
     6001 aggcgcgtgt aagttacagg caagcgatcc gtctaagaaa ccattattat catgacatta
     6061 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     6121 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     6181 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     6241 aactatgcgg catcagagca gattgtactg agagtgcacc atagatcacg acattactat
     6301 atatataata taggaagcat ttaatagaac agcatcgtaa tatatgtgta ctttgcagtt
     6361 atgacgccag atggcagtag tggaagatat tctttattga aaaatagctt gtcaccttac
     6421 gtacaatctt gatccggagc ttttcttttt ttgccgatta agaattaatt cggtcgaaaa
     6481 aagaaaagga gagggccaag agggagggca ttggtgacta ttgagcacgt gagtatacgt
     6541 gattaagcac acaaaggcag cttggagtat gtctgttatt aatttcacag gtagttctgg
     6601 tccattggtg aaagtttgcg gcttgcagag cacagaggcc gcagaatgtg ctctagattc
     6661 cgatgctgac ttgctgggta ttatatgtgt gcccaataga aagagaacaa ttgacccggt
     6721 tattgcaagg aaaatttcaa gtcttgtaaa agcatataaa aatagttcag gcactccgaa
     6781 atacttggtt ggcgtgtttc gtaatcaacc taaggaggat gttttggctc tggtcaatga
     6841 ttacggcatt gatatcgtcc aactgcatgg agatgagtcg tggcaagaat accaagagtt
     6901 cctcggtttg ccagttatta aaagactcgt atttccaaaa gactgcaaca tactactcag
     6961 tgcagcttca cagaaacctc attcgtttat tcccttgttt gattcagaag caggtgggac
     7021 aggtgaactt ttggattgga actcgatttc tgactgggtt ggaaggcaag agagccccga
     7081 aagcttacat tttatgttag ctggtggact gacgccagaa aatgttggtg atgcgcttag
     7141 attaaatggc gttattggtg ttgatgtaag cggaggtgtg gagacaaatg gtgtaaaaga
     7201 ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa taggttatta ctgagtagta
     7261 tttatttaag tattgtttgt gcacttgccg atctatgcgg tgtgaaatac cgcacagatg
     7321 cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg
     7381 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     7441 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     7501 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     7561 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     7621 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     7681 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     7741 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     7801 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     7861 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     7921 ggttttccca gtcacgacgt t
//
