LOCUS       sequence-213746-        7824 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Truncated Hsh155 (aa 103-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 103-972
FEATURES             Location/Qualifiers
     source          1..7824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..29
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10..28
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3190..3210)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3190..3208)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3229..3245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3229..3245)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3242..3264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3253..3269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3277..3283,3284..3301,3302..3307))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3322..3343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3460..3477)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3631..4219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3711..3730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4390..5181,5182..5250))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5013..5032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5251..5355)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    5392..5895
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     5936..5954
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5992..6014)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6114..6133
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6154..6434
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6435..7109
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7212..7667)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7349..7370)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7561..7580
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7793..7815
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7807..7824
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7808..7824
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gagcgcgcgt aatacgactc actatagggc gaattgggta ccgggccccc ccctcgagca
       61 tgtactttcc gcctaactac catcacctgt ctttatttat tccgctgaca gatatgcaca
      121 accaattatc gtctcttgaa agctttttag acgtgattgc tgttatttgg cacgttgtta
      181 gtatttaagc ttattcgggt ttgcattgat atttgcccga gcagaaatct ccttctcgat
      241 gagttagaac tgaaacatct cttcaattac ttatacataa taaacataga aagccaggac
      301 ctgtcatcat ggtaaaaggg tacgagccac ctgatgaatc ttcaacagcg gtcaaagaaa
      361 actctgatag tgctttggtt aatgtagaag gaatccatga tttgatgttt tttaaacctt
      421 ctgaccataa atatttcgcc gacgttatat ccaaaaaacc catagatgag ttaaataagg
      481 atgaaaagaa agaaagaacc ctgtcgatgt tacttctgaa aataaaaaat ggcaatacag
      541 cgagtaggag gacttctatg cggattttaa ccgataaagc tgttacattt ggtccagaaa
      601 tgatatttaa ccgcttattg cccatattac tagacagaag tttggaagac caggaaagac
      661 atctaatgat caagaccata gatcgcgtcc tttaccagct aggagattta acaaaacctt
      721 acgttcacaa aatacttgta gtagctgccc cattgctaat agatgaagat ccaatggttc
      781 gttcaacagg ccaggagata atcaccaatc tgtctacagt cgctggccta aaaaccattc
      841 taacagtaat gagacctgat attgagaatg aggatgagta tgttagaaat gtaacttcaa
      901 gagcggcggc tgtggtggca aaagcccttg gtgttaatca attgcttcct ttcatcaatg
      961 cagcatgcca ctcacggaag tcttggaaag caaggcatac tggcataaaa attgtgcagc
     1021 aaataggtat acttttggga ataggtgtct tgaatcatct tactgggctg atgagttgta
     1081 tcaaagactg cctaatggat gatcacgttc cggtgcgaat tgttacagca catactttat
     1141 caacactagc agaaaattct tatccatatg gtatcgaagt tttcaacgtt gtactggagc
     1201 cattatggaa gggtataaga agtcatcgtg gtaaagtgct atcttctttt ttaaaagctg
     1261 ttggatctat gatcccattg atggacccag agtatgccgg ttattatacc acagaagcaa
     1321 tgagaataat tagacgtgaa ttcgactctc ccgatgatga aatgaaaaag accattcttt
     1381 tagtattaca aaaatgtagc gctgtagaat ccataacgcc aaaatttctg agagaagaaa
     1441 tcgcgccaga attctttcaa aaattttggg tcagacgagt ggcattggat agaccattaa
     1501 acaaagttgt tacatatact actgttacat tagcaaagaa attaggttgt tcttatacca
     1561 ttgataaatt attaacacca ttgagagatg aagctgagcc gttcagaaca atggctgtcc
     1621 atgctgttac tagaacagta aacttattag ggacagcaga tttagatgag agattagaaa
     1681 cgaggcttat tgatgcgctc ttgatagcat ttcaagaaca aacaaatagt gattctataa
     1741 tctttaaagg gtttggggca gtgacggtgt cacttgacat ccgtatgaaa ccgtttttag
     1801 cacccattgt gagtacgatt ttaaatcatt taaagcataa aactcccttg gttcgtcaac
     1861 atgcagcaga tctatgtgcc atactaatac cagtcatcaa gaactgtcac gaattcgaaa
     1921 tgttgaataa attgaacatt atattgtatg aatccttagg cgaagtatat ccagaggtat
     1981 taggttctat aatcaacgct atgtactgta ttactagcgt aatggatttg gataaactac
     2041 aacctcctat taatcaaatt ttaccaacct taactccaat tttaagaaac aagcatagaa
     2101 aagtcgaagt aaacactatc aaatttgttg gacttattgg taaattagca cctacctatg
     2161 cacctcctaa agaatggatg agaatatgtt ttgaactatt agaacttttg aaaagtacaa
     2221 ataaagaaat aagaagatca gcaaatgcca cgtttggatt cattgctgaa gcaattggac
     2281 ctcacgatgt tcttgttgcg ttattgaaca atttaaaggt tcaagaacgt cagttacgtg
     2341 tatgcacggc tgttgctatc ggtatcgttg ctaaagtgtg tggtccttac aacgtgctgc
     2401 cagtaatcat gaatgaatac acaacaccag agactaatgt tcaaaacggt gttctcaagg
     2461 ccatgtcatt catgtttgag tacattggta acatgtctaa ggattacata tacttcataa
     2521 caccattatt agaagatgca ctcaccgata gggatttagt tcatcgtcag acagcgtcga
     2581 acgtaataac ccacttagct ttaaactgtt caggtacagg tcacgaagat gccttcattc
     2641 atttaatgaa tcttctaata cccaatattt ttgaaacttc accacatgct attatgcgta
     2701 ttttagaagg tttggaggca ctgagtcaag ctcttggccc tggactattc atgaactaca
     2761 tatgggcagg attgttccat ccagcgaaga atgtaaggaa agcattttgg agggtgtata
     2821 ataacatgta cgtaatgtac caggacgcca tggtaccttt ttaccccgtt acaccagaca
     2881 acaatgaaga atatatagaa gaactggatt tagttctgtg aatatatata gagcatataa
     2941 ataaccacaa cttgtaagaa tattttactt gacacaccta tatcatcacg tcacgatgcc
     3001 ttcatagatt atgatgaaat ataaacagaa cctgctattg gaaaaaaaag ggcaggaaaa
     3061 aattttgtga aagtgagtaa aagaaagatg aagagcgaga catggataat aagaaataga
     3121 tgtcactaat ggacaattgg aagactgata tggaaagtta cgatgaagga ggagctccag
     3181 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     3241 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3301 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3361 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3421 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3481 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3541 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3601 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3661 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3721 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3781 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3841 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3901 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3961 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4021 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4081 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4141 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4201 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4261 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4321 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4381 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4441 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4501 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4561 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4621 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4681 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4741 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4801 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4861 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4921 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4981 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     5041 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     5101 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     5161 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     5221 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     5281 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5341 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcctttt catcacgtgc
     5401 tataaaaata attataattt aaatttttta atataaatat ataaattaaa aatagaaagt
     5461 aaaaaaagaa attaaagaaa aaatagtttt tgttttccga agatgtaaaa gactctaggg
     5521 ggatcgccaa caaatactac cttttatctt gctcttcctg ctctcaggta ttaatgccga
     5581 attgtttcat cttgtctgtg tagaagacca cacacgaaaa tcctgtgatt ttacatttta
     5641 cttatcgtta atcgaatgta tatctattta atctgctttt cttgtctaat aaatatatat
     5701 gtaaagtacg ctttttgttg aaatttttta aacctttgtt tatttttttt tcttcattcc
     5761 gtaactcttc taccttcttt atttactttc taaaatccaa atacaaaaca taaaaataaa
     5821 taaacacaga gtaaattccc aaattattcc atcattaaaa gatacgaggc gcgtgtaagt
     5881 tacaggcaag cgatccgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     5941 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     6001 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     6061 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     6121 agagcagatt gtactgagag tgcaccatag atcacgacat tactatatat ataatatagg
     6181 aagcatttaa tagaacagca tcgtaatata tgtgtacttt gcagttatga cgccagatgg
     6241 cagtagtgga agatattctt tattgaaaaa tagcttgtca ccttacgtac aatcttgatc
     6301 cggagctttt ctttttttgc cgattaagaa ttaattcggt cgaaaaaaga aaaggagagg
     6361 gccaagaggg agggcattgg tgactattga gcacgtgagt atacgtgatt aagcacacaa
     6421 aggcagcttg gagtatgtct gttattaatt tcacaggtag ttctggtcca ttggtgaaag
     6481 tttgcggctt gcagagcaca gaggccgcag aatgtgctct agattccgat gctgacttgc
     6541 tgggtattat atgtgtgccc aatagaaaga gaacaattga cccggttatt gcaaggaaaa
     6601 tttcaagtct tgtaaaagca tataaaaata gttcaggcac tccgaaatac ttggttggcg
     6661 tgtttcgtaa tcaacctaag gaggatgttt tggctctggt caatgattac ggcattgata
     6721 tcgtccaact gcatggagat gagtcgtggc aagaatacca agagttcctc ggtttgccag
     6781 ttattaaaag actcgtattt ccaaaagact gcaacatact actcagtgca gcttcacaga
     6841 aacctcattc gtttattccc ttgtttgatt cagaagcagg tgggacaggt gaacttttgg
     6901 attggaactc gatttctgac tgggttggaa ggcaagagag ccccgaaagc ttacatttta
     6961 tgttagctgg tggactgacg ccagaaaatg ttggtgatgc gcttagatta aatggcgtta
     7021 ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt aaaagactct aacaaaatag
     7081 caaatttcgt caaaaatgct aagaaatagg ttattactga gtagtattta tttaagtatt
     7141 gtttgtgcac ttgccgatct atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     7201 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     7261 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     7321 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     7381 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     7441 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     7501 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     7561 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     7621 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
     7681 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     7741 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     7801 cgacgttgta aaacgacggc cagt
//
