LOCUS       sequence-214275-        5244 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Luciferase reporter for the human BRN2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Zeng H, Jorapur A, Shain AH, Lang UE, Torres R, Zhang Y, McNeal AS, 
            Botton T, Lin J, Donne M, Bastian IN, Yu R, North JP, Pincus L, 
            Ruben BS, Joseph NM, Yeh I, Bastian BC, Judson RL
  TITLE     Bi-allelic Loss of CDKN2A Initiates Melanoma Invasion via BRN2 
            Activation.
  JOURNAL   Cancer Cell. 2018 Jul 9;34(1):56-68.e9. doi: 
            10.1016/j.ccell.2018.05.014.
  PUBMED    29990501
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.05"; journalName: "Cancer Cell"; date: 
            "2018-07-9- 9"; volume: "34"; issue: "1"; pages: "56-68.e9"
COMMENT     Sequence Label: pGL410-BRN2p
FEATURES             Location/Qualifiers
     source          1..5244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1096..1105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1102..2754
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2798..2919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2809..2828)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2863..2882
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3167..3184)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3338..3926)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3418..3437)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4126..4917,4918..4986))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5091..5139
                     /note="synthetic polyadenylation signal"
     misc_feature    5153..5244
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5193..5212
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgaagc cttgatggga atgacttaca
       61 agccgcggtg cgggctgagc ctgcctctct cggggttctt tccccaaggg agtgggagac
      121 aattgggcct ggaacctaaa aggaagagag cctgtgctag ccgcggggta gaggggaagg
      181 agaagagctt tgggcagaag tgtccgcaca ccctggagca agagacactt gtgcactgaa
      241 attctaaccg tgggatgcaa ggagagttga acaggggaac aggtctggct cttggctcta
      301 ggtttaagag atacacactg tgcgtgtgtc cctaggtgaa taaaatccga ctcccgctgg
      361 ctcggtgcga cccagctttt cccttacccc ctccgtttcc ccatcttaaa gccctattgg
      421 gaaacgaaag tggacaccac tgcctcccgc cccccacccc aatccaagtc ttccaagtgt
      481 ctgtggccga taagagcacc gggaccgccc ccccgccggt cttctctgcc tgggagacag
      541 atggggggcg gggcccatcc gagagagggc ggaggaggcc ccgggtgagg aagaagcggg
      601 ggggtgagaa ctgagagaat ctgaatcggg aggcgaaggg gacggggagg agggctagga
      661 ggactccgag cccgggggag ggggagggag tagctctgcg ccaatcagtg gcgccggcct
      721 gggaggttgc tagcggtatc cacgtaaatc aaagggcgcg gagccaatgg gagggggcgg
      781 agggggcggg gtccaggcgc gtgccgctgc gagccggcgc tgccaagagg gcgggagaga
      841 gctggagaga gcagggagag gggggagcgc cgagctagtc agagagtgag cgagagcgag
      901 aaggagggag aggaggagaa agagagcgag ggcgggcggg aggcggcggc ggcggcagca
      961 gcagcagtaa tagcaggagc agcaacagaa ggcgtcggag cgggcgtcgg agctgcccgc
     1021 tgtgggagag agaggagaca gaaagagcga tctggcctcg gcggccaagc ttggcaatcc
     1081 ggtactgttg gtaaagccac catggaagat gccaaaaaca ttaagaaggg cccagcgcca
     1141 ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac
     1201 gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac
     1261 gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat
     1321 acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg
     1381 ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag
     1441 ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg
     1501 caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat
     1561 agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca
     1621 cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc
     1681 ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc
     1741 accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc
     1801 gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg
     1861 ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc
     1921 ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc
     1981 ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc
     2041 agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta
     2101 ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc
     2161 gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg
     2221 gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt
     2281 ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac
     2341 aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc
     2401 atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa
     2461 ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc
     2521 gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg
     2581 accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc
     2641 ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc
     2701 aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt gtaataattc
     2761 tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga
     2821 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     2881 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     2941 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     3001 aaatgtggta aaatcgataa ggatccgtcg accgatgccc ttgagagcct tcaacccagt
     3061 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
     3121 tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact
     3181 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3241 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3301 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3361 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3421 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3481 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3541 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3601 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3661 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3721 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3781 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3841 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3901 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3961 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4021 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4081 aaacttggtc tgacagcggc cgcaaatgct aaaccactgc agtggttacc agtgcttgat
     4141 cagtgaggca ccgatctcag cgatctgcct atttcgttcg tccatagtgg cctgactccc
     4201 cgtcgtgtag atcactacga ttcgtgaggg cttaccatca ggccccagcg cagcaatgat
     4261 gccgcgagag ccgcgttcac cggcccccga tttgtcagca atgaaccagc cagcagggag
     4321 ggccgagcga agaagtggtc ctgctacttt gtccgcctcc atccagtcta tgagctgctg
     4381 tcgtgatgct agagtaagaa gttcgccagt gagtagtttc cgaagagttg tggccattgc
     4441 tactggcatc gtggtatcac gctcgtcgtt cggtatggct tcgttcaact ctggttccca
     4501 gcggtcaagc cgggtcacat gatcacccat attatgaaga aatgcagtca gctccttagg
     4561 gcctccgatc gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg taatggcagc
     4621 actacacaat tctcttaccg tcatgccatc cgtaagatgc ttttccgtga ccggcgagta
     4681 ctcaaccaag tcgttttgtg agtagtgtat acggcgacca agctgctctt gcccggcgtc
     4741 tatacgggac aacaccgcgc cacatagcag tactttgaaa gtgctcatca tcgggaatcg
     4801 ttcttcgggg cggaaagact caaggatctt gccgctattg agatccagtt cgatatagcc
     4861 cactcttgca cccagttgat cttcagcatc ttttactttc accagcgttt cggggtgtgc
     4921 aaaaacaggc aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat
     4981 gctcatactc gtcctttttc aatattattg aagcatttat cagggttact agtacgtctc
     5041 tcaaggataa gtaagtaata ttaaggtacg ggaggtattg gacaggccgc aataaaatat
     5101 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg
     5161 ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa
     5221 gtgcaggtgc cagaacattt ctct
//
