LOCUS       sequence-214531-        8681 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses 3XHA-TMEM192 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Wyant GA, Abu-Remaileh M, Wolfson RL, Chen WW, Freinkman E, Danai 
            LV, Vander Heiden MG, Sabatini DM
  TITLE     mTORC1 Activator SLC38A9 Is Required to Efflux Essential Amino Acids
            from Lysosomes and Use Protein as a Nutrient.
  JOURNAL   Cell. 2017 Oct 19;171(3):642-654.e12. doi: 
            10.1016/j.cell.2017.09.046.
  PUBMED    29053970
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09"; journalName: "Cell"; date: "2017-10-19- 
            19"; volume: "171"; issue: "3"; pages: "642-654.e12"
COMMENT     Sequence Label: pLJC6-3XHA-TMEM192
FEATURES             Location/Qualifiers
     source          1..8681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1824..3035
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2872..2891
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3057..3066
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3887..3913
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3926..3952
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3965..3991
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    4048..4165
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4214..4724
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4643..4661
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4733..5128
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5200..5433
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5505..5626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5542..5561)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5596..5615
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5666..5801
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5821..5840)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5822..5840)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5850..5867)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5850..5866)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5859..5881)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6008..6463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6095..6114)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6305..6326
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6489..6593
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6594..6662,6663..7454)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6812..6831)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7625..8213
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8114..8133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8367..8384
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8501..8522
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8537..8542,8543..8560,8561..8567)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8575..8591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8580..8602
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8599..8615
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8599..8615
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8634..8654
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8636..8654
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcag cagctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     1861 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     1921 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     1981 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     2041 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     2101 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     2161 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     2221 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     2281 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     2341 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     2401 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     2461 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     2521 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     2581 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     2641 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     2701 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     2761 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     2821 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     2881 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     2941 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3001 ctaaattctg gccgtttttg gcttttttgt tagacgaagc gctagcgcta ccggtaccat
     3061 ggcggcgggg ggcaggatgg aggacggttc cttggatatc acccagagta ttgaagacga
     3121 cccacttctg gatgcccagc ttctcccaca ccactcatta caagctcact ttagaccccg
     3181 attccatcct cttcctacag tcatcatagt gaatcttctg tggtttattc atctcgtgtt
     3241 tgttgtttta gcatttttaa caggtgtgct ttgttcttat cctaatccaa atgaggacaa
     3301 gtgcccagga aattacacaa acccattgaa agttcagacg gttataatcc ttgggaaagt
     3361 tattttgtgg attctccatt tactccttga atgctacatc cagtatcacc acagcaaaat
     3421 cagaaaccga ggctataact tgatctaccg atcaacaagg catctcaaga gacttgcgtt
     3481 gatgatacag tcctctggca acacagtgct tctcctcata ctgtgcatgc agcactcctt
     3541 cccagagcct ggcagattgt atcttgacct cattctggcc atcttggcac tggaactcat
     3601 ctgttccctg atatgtctcc tcatttacac agtgaaaatc cggagattta ataaagctaa
     3661 accagagcct gatatacttg aagaagaaaa aatctatgct taccccagca atattacctc
     3721 ggagactgga ttcagaacta tttcaagcct agaagaaatt gttgaaaagc aaggagacac
     3781 cattgaatac ctgaagcgac acaatgcgct gctgagtaag cgattgttgg ctctcacttc
     3841 ctcagacctg ggctgtcagc caagtagaac gggaggtgga ggtagttatc cctatgacgt
     3901 gcctgattac gccggcacag gatcctaccc ctatgatgtg cctgactacg ctggcagcgc
     3961 cggataccct tatgatgtgc ctgattatgc tggagggagc ggctaagaat tctcgacctc
     4021 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     4081 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     4141 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     4201 gccgcggctc gagggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg
     4261 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
     4321 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
     4381 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
     4441 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
     4501 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
     4561 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
     4621 gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg
     4681 cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat ccatggttcc
     4741 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
     4801 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
     4861 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
     4921 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
     4981 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
     5041 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
     5101 gccctctggt tatgtgtggg agggctaagg tacctttaag accaatgact tacaaggcag
     5161 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5221 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     5281 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5341 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5401 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     5461 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     5521 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5581 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     5641 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5701 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5761 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     5821 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     5881 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5941 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     6001 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6061 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6121 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6181 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6241 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6301 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6361 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6421 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     6481 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6541 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6601 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6661 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6721 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6781 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6841 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6901 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6961 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7021 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7081 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7141 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7201 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7261 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7321 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7381 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7441 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7501 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7561 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7621 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7681 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7741 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7801 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7861 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7921 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7981 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8041 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8101 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8161 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8221 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8281 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8341 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8401 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8461 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     8521 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     8581 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     8641 accctcacta aagggaacaa aagctggagc tgcaagctta a
//
