LOCUS       sequence-214632-        8830 bp    DNA     circular SYN 14-APR-2025
DEFINITION  3rd generation lentiviral vector. eFFly luciferase for in vivo 
            Imaging, mCherry for fluorescent detection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Ebinger S, Ozdemir EZ, Ziegenhain C, Tiedt S, Castro Alves C, 
            Grunert M, Dworzak M, Lutz C, Turati VA, Enver T, Horny HP, Sotlar 
            K, Parekh S, Spiekermann K, Hiddemann W, Schepers A, Polzer B, 
            Kirsch S, Hoffmann M, Knapp B, Hasenauer J, Pfeifer H, 
            Panzer-Grumayer R, Enard W, Gires O, Jeremias I
  TITLE     Characterization of Rare, Dormant, and Therapy-Resistant Cells in 
            Acute Lymphoblastic Leukemia.
  JOURNAL   Cancer Cell. 2016 Dec 12;30(6):849-862. doi: 
            10.1016/j.ccell.2016.11.002. Epub 2016 Dec 1.
  PUBMED    27916615
REFERENCE   2  (bases 1 to 8830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.11.002"; journalName: "Cancer Cell"; date: 
            "2016-12-12- 12"; volume: "30"; issue: "6"; pages: "849-862"
COMMENT     Sequence Label: pCDH-EF1a-eFFly-mCherry
FEATURES             Location/Qualifiers
     source          1..8830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2171
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2184..2452
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4148..4201
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4208..4918
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4353..4371)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4621..4640
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4815..4838
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    4925..5513
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4978..4998)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5396..5407)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5587..5820
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5892..6013
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5929..5948)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5983..6002
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6032..6167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6205..6221)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6205..6221)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6218..6240)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6229..6245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6253..6259,6260..6277,6278..6283))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6298..6319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6436..6453)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6607..7195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7366..8157,8158..8226))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7989..8008
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8227..8331)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8399..8417
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8455..8477)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8577..8596
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8790..8812
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8804..8821
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8805..8821
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     2461 gaccggcgcc tactctagag ctagcgaatt catggaagat gccaagaaca tcaagaaagg
     2521 ccctgccccc ttctaccccc tggaagatgg cacagccggc gagcagctgc acaaggccat
     2581 gaagagatac gccctggtgc ccggcaccat cgccttcacc gacgcccaca tcgaggtgga
     2641 catcacctac gccgagtatt tcgagatgag cgtgcggctg gccgaggcca tgaaacgcta
     2701 cggcctgaac accaaccacc ggatcgtggt gtgcagcgag aacagcctgc agttcttcat
     2761 gcccgtgctg ggcgccctgt tcatcggcgt ggccgtggcc cctgccaacg acatctacaa
     2821 cgagcgggag ctgctgaaca gcatgggcat cagccagccc accgtggtgt tcgtgagcaa
     2881 gaagggcctg cagaaaatcc tgaacgtgca gaagaagctg cccatcatcc agaaaatcat
     2941 catcatggac agcaagaccg actaccaggg cttccagagc atgtacacct tcgtgaccag
     3001 ccacctgccc cctggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa
     3061 gaccatcgcc ctgatcatga acagcagcgg cagcaccggc ctgcctaaag gcgtggccct
     3121 gcctcaccgg accgcctgcg tgcggttcag ccacgcccgg gaccccatct tcggcaacca
     3181 gatcatcccc gacaccgcca tcctgagcgt ggtgcccttc caccacggct tcggcatgtt
     3241 caccaccctg ggctacctga tctgcggctt ccgggtggtg ctgatgtacc ggttcgagga
     3301 agagctgttc ctgcggagcc tgcaggacta caagatccag agcgccctgc tggtgcccac
     3361 cctgttcagc tttttcgcca agagcaccct gatcgacaag tacgacctga gcaacctgca
     3421 cgagatcgcc agcggcggag cccccctgtc caaagaagtg ggcgaagccg tcgccaagcg
     3481 gttccacctg cccggcatcc ggcagggcta tggcctgacc gagaccacaa gcgccattct
     3541 gatcaccccc gagggcgacg acaagcctgg cgccgtgggc aaggtggtgc ctttcttcga
     3601 ggccaaggtg gtggacctgg acaccggcaa gaccctgggc gtgaaccagc ggggcgagct
     3661 gtgcgtgagg ggccccatga tcatgagcgg ctacgtgaac aaccccgagg ccaccaacgc
     3721 cctgattgac aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga
     3781 gcacttcttc atcgtggacc ggctgaagag cctgatcaag tacaagggct accaggtggc
     3841 cccagccgag ctggaaagca tcctgctgca gcaccccaac atcttcgatg ccggggtggc
     3901 cggactgccc gacgacgatg ccggcgagct gcctgccgcc gtggtggtgc tggaacacgg
     3961 caaaaccatg accgagaaag aaatcgtgga ctacgtggcc agccaggtga ccaccgccaa
     4021 gaaactgaga ggcggcgtgg tgtttgtgga cgaggtgccc aagggcctga caggcaagct
     4081 ggacgcccgg aagatccggg agatcctgat caaggccaag aagggcggca agggatccgc
     4141 ggccgctgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     4201 tatgcatatg gtgagcaagg gcgaggagga caacatggcc atcatcaagg agttcatgcg
     4261 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     4321 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
     4381 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     4441 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     4501 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     4561 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     4621 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     4681 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     4741 cgacgccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     4801 caacgtcaac atcaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     4861 gtacgagcgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaagt
     4921 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4981 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     5041 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     5101 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     5161 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     5221 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5281 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     5341 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5401 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5461 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac
     5521 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5581 ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg
     5641 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5701 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5761 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5821 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     5881 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5941 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6001 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc
     6061 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6121 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag
     6181 agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6241 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6301 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6361 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6421 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6481 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6541 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6601 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6661 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6721 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6781 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6841 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6901 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6961 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7021 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     7081 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7141 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7201 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7261 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7321 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7381 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7441 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7501 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7561 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7621 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7681 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7741 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7801 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7861 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7921 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7981 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8041 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8101 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8161 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8221 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8281 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8341 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     8401 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     8461 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     8521 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     8581 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     8641 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     8701 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     8761 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     8821 tgccaagctg
//
