LOCUS       sequence-214860-        1089 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Single pathway reporter; hedgehog promoter-driven expression of 
            mTurquoise2; MultiSite Gateway entry vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M
  TITLE     Luminescent and fluorescent triple reporter plasmid constructs for 
            Wnt, Hedgehog and Notch pathway.
  JOURNAL   PLoS One. 2019 Dec 20;14(12):e0226570. doi: 
            10.1371/journal.pone.0226570. eCollection 2019.
  PUBMED    31860685
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-12-20"; volume: "14(12):e0226570. doi"; pages: " 
            10.1371/journal.pone.0226570. eCollection 2019"
COMMENT     Sequence Label: pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(13..137)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 aagtagttga ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
       61 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact
      121 tttctataca aagttgggag acccaagctt ggtaccgagc tcttacgcgt gctagagctt
      181 gcatgcctgc aggtcgacac actggcgcac tatccagcac gctgtcatta ttggtgcagg
      241 cctgggaatg cgagccggac ctcccagtat ttgctgcagc ttgaggccaa ggtgcctctg
      301 aaaggctttt ctttgaggaa tttcagaaat tgtttaagcg cttaaaaaaa tattacattt
      361 ccccccgtgt ctaccgcgag gcaaatgttc gtgtgtgtgc gtgcgcgcgc gtgtgtgcgc
      421 gcaaaggcat cccacaaaaa gaagacactg ttgaaaagaa aaggaataag agagaaagga
      481 gggggagcat tccttaatgg aagtattgca tgcgagaagg ttggcgctct cagaggctgc
      541 cccgaagccg aggatgcaca cactgggttg cctacctggg tggtctctct actttggtga
      601 gctgttgcta agcagctaat aatagtcatg tttgctgagt aatttctgcc ctccgcgagc
      661 caatcgcgtc gcagaaaccc aagccatctg acagccccgg gggcgggagc tccagccttt
      721 ttctctttcg ctcgctctct ccccctccac cccctcccct cccctcctcc tcttctttct
      781 ccaaatggag agagttcttt tttttcttct tccatctcat ctattgaatc aaggagctgc
      841 tgcggccgct gcccgctgca cacacacaga gcggagtccc caggtcccgg gagcgagaga
      901 ggcgcgctgc acggggacag aatggtccag cgggttgccg aggarcacaa raaagcagag
      961 tccgggaccg agcagccacc gcgaacccag cagccagagc ccgagcagcc cgagcagcag
     1021 ctcctggagc cgccaccgcc agcagcagcc accgcgggag cagcggcagc tgcggttgcc
     1081 cgggcccgg
//
