LOCUS       sequence-215128-        8313 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Electrophysiology in HEK cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Chang A, Abderemane-Ali F, Hura GL, Rossen ND, Gate RE, Minor DL Jr.
  TITLE     A Calmodulin C-Lobe Ca(2+)-Dependent Switch Governs Kv7 Channel 
            Function.
  JOURNAL   Neuron. 2018 Feb 21;97(4):836-852.e6. doi: 
            10.1016/j.neuron.2018.01.035. Epub 2018 Feb 8.
  PUBMED    29429937
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2018.01.035"; journalName: "Neuron"; date: 
            "2018-02-21- 21"; volume: "97"; issue: "4"; pages: "836-852.e6"
COMMENT     Sequence Label: Kv7.4 (1-695)/pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..8313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3044..3064
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             join(3065..3067,3068..3778)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3065..3067,3068..3778)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3107..3128)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3368..3387)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3715..3736
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3779..3802
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     promoter        complement(3866..3884)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3867..3884)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3904..3921)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3910..4134
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4180..4608
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4267..4286)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4477..4498
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4617..4637)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4622..4951
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4802..4937
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5018..5812
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5072..5091)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5682..5701
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5986..6107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6023..6042)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6077..6096
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6156..6172)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6156..6172)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6169..6191)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6180..6196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6204..6210,6211..6228,6229..6234))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6249..6270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6387..6404)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6558..7146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6638..6657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7317..8108,8109..8177))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7940..7959
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8178..8282)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccttgggtac ttccaatcca tttgatggcc gaggcccccc cgcgccgcct
      961 cggcctgggt cccccgcccg gggacgcccc ccgcgcggag ctagtggcgc tcacggccgt
     1021 gcagagcgaa cagggcgagg cgggcggggg cggctccccg cgccgcctcg gcctcctggg
     1081 cagccccctg ccgccgggcg cgcccctccc tgggccgggc tccggctcgg gctccgcctg
     1141 cggccagcgc tcctcggccg cgcacaagcg ctaccgccgc ctgcagaact gggtctacaa
     1201 cgtgctggag cggccccgcg gctgggcctt cgtctaccac gtcttcatat ttttgctggt
     1261 cttcagctgt ctggtgctgt ctgtgctgtc cactatccag gagcaccagg aacttgccaa
     1321 cgagtgtctc ctcatcttgg aattcgtgat gatcgtggtt ttcggcttgg agtacatcgt
     1381 ccgggtctgg tccgccggat gctgctgccg ctaccgagga tggcagggtc gcttccgctt
     1441 tgccagaaag cccttctgtg tcatcgactt catcgtgttc gtggcctcgg tggccgtcat
     1501 cgccgcgggt acccagggca acatcttcgc cacgtccgcg ctgcgcagca tgcgcttcct
     1561 gcagatcctg cgcatggtgc gcatggaccg ccgcggcggc acctggaagc tgctgggctc
     1621 agtggtctac gcgcatagca aggagctgat caccgcctgg tacatcgggt tcctggtgct
     1681 catcttcgcc tccttcctgg tctacctggc cgagaaggac gccaactccg acttctcctc
     1741 ctacgccgac tcgctctggt gggggacgat tacattgaca accatcggct atggtgacaa
     1801 gacaccgcac acatggctgg gcagggtcct ggctgctggc ttcgccttac tgggcatctc
     1861 tttctttgcc ctgcctgccg gcatcctagg ctccggcttt gccctgaagg tccaggagca
     1921 gcaccggcag aagcacttcg agaagcggag gatgccggca gccaacctca tccaggctgc
     1981 ctggcgcctg tactccaccg atatgagccg ggcctacctg acagccacct ggtactacta
     2041 tgacagtatc ctcccatcct tcagagagct ggccctcttg tttgagcacg tgcaacgggc
     2101 ccgcaatggg ggcctacggc ccctggaggt gcggcgggcg ccggtacccg acggagcacc
     2161 ctcccgttac ccgcccgttg ccacctgcca ccggccgggc agcacctcct tctgccctgg
     2221 ggaaagcagc cggatgggca tcaaagaccg catccgcatg ggcagctccc agcggcggac
     2281 gggtccttcc aagcagcatc tggcacctcc aacaatgccc acctccccaa gcagcgagca
     2341 ggtgggtgag gccaccagcc ccaccaaggt gcaaaagagc tggagcttca atgaccgcac
     2401 ccgcttccgg gcatctctga gactcaaacc ccgcacctct gctgaggatg ccccctcaga
     2461 ggaagtagca gaggagaaga gctaccagtg tgagctcacg gtggacgaca tcatgcctgc
     2521 tgtgaagaca gtcatccgct ccatcaggat tctcaagttc ctggtggcca aaaggaaatt
     2581 caaggagaca ctgcgaccgt acgacgtgaa ggacgtcatt gagcagtact cagcaggcca
     2641 cctggacatg ctgggccgga tcaagagcct gcaaactcgg gtggaccaaa ttgtgggtcg
     2701 ggggcccggg gacaggaagg cccgggagaa gggcgacaag gggccctccg acgcggaggt
     2761 ggtggatgaa atcagcatga tgggacgcgt ggtcaaggtg gagaagcagg tgcagtccat
     2821 cgagcacaag ctggacctgc tgttgggctt ctattcgcgc tgcctgcgct ctggcacctc
     2881 ggccagcctg ggcgccgtgc aagtgccgct gttcgacccc gacatcacct ccgactacca
     2941 cagccctgtg gaccacgagg acatctccgt ctccgcacag acgctcagca tctcccgctc
     3001 ggtcagcacc aacatggacg gcaaattggg attaattaat ctcgaaaacc tgtacttcca
     3061 atccgtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     3121 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     3181 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     3241 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3301 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3361 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3421 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3481 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3541 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3601 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3661 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     3721 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagca
     3781 tcaccatcac catcaccatc actaaggatc ctgcagatat ccatcacact ggcggccgct
     3841 cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
     3901 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     3961 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     4021 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4081 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     4141 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     4201 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4261 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4321 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4381 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4441 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4501 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4561 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     4621 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4681 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4741 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4801 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4861 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     4921 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     4981 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     5041 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     5101 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     5161 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     5221 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5281 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     5341 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5401 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5461 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     5521 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     5581 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     5641 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     5701 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     5761 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     5821 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     5881 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5941 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     6001 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6061 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     6121 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6181 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6241 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6301 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6361 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6421 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6481 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6541 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6601 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6661 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6721 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6781 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6841 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6901 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6961 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7021 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7081 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7141 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7201 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7261 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7321 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7381 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7441 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7501 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7561 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7621 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7681 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7741 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7801 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7861 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7921 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7981 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8041 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8101 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8161 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8221 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8281 cgcacatttc cccgaaaagt gccacctgac gtc
//
