LOCUS       sequence-215243-        9003 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of mouse Cebpa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Montagner S, Leoni C, Emming S, Della Chiara G, Balestrieri C, 
            Barozzi I, Piccolo V, Togher S, Ko M, Rao A, Natoli G, Monticelli S
  TITLE     TET2 Regulates Mast Cell Differentiation and Proliferation through 
            Catalytic and Non-catalytic Activities.
  JOURNAL   Cell Rep. 2016 May 17;15(7):1566-79. doi: 
            10.1016/j.celrep.2016.04.044. Epub 2016 May 5.
  PUBMED    27160912
REFERENCE   2  (bases 1 to 9003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.04.044"; journalName: "Cell Rep"; date: 
            "2016-05-17- 17"; volume: "15"; issue: "7"; pages: "1566-79"
COMMENT     Sequence Label: pScalps_Puro_mCebpa
FEATURES             Location/Qualifiers
     source          1..9003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(239..259)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        244..573
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      424..559
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     486..505
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        585..964
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        966..1164
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1119..1139
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1182..1362
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1409..1534
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2031..2264
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2448..2492
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2790..2907
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3044..3451
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     3511..3531
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3589..3613
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4691..4715)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5284..5883
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5284..5303)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5780..5800
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5893..6481
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5946..5966)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6364..6375)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6999..7179
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7250..7838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7330..7349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8009..8800,8801..8869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8632..8651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8870..8974)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgctgtgg
      241 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      301 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
      361 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
      421 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
      481 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
      541 ggaggctttt ttggaggcct aggcttttgc aaaaagctcg cgttgacatt gattattgac
      601 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      901 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      961 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     1021 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     1081 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     1141 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
     1201 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     1261 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     1321 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     1381 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     1441 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     1501 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     1561 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     1621 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     1681 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1741 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1801 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     1861 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat
     1921 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     1981 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2041 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2101 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2161 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2221 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2281 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2341 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaatt aacaattaca
     2401 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     2461 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     2521 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     2581 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     2641 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     2701 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     2761 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2821 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2881 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2941 tggttaatta actgcagccc cgataaaata aaagatttta tttagtctcc agaaaaaggg
     3001 gggaatgaaa gaccccacct gtaggtttgg caagctagct gcagtaacgc cattttgcaa
     3061 ggcatggaaa aataccaaac caagaataga gaagttcaga tcaagggcgg gtacatgaaa
     3121 atagctaacg ttgggccaaa caggatatct gcggtgagca gtttcggccc cggcccgggg
     3181 ccaagaacag atggtcaccg cagtttcggc cccggcccga ggccaagaac agatggtccc
     3241 cagatatggc ccaaccctca gcagtttctt aagacccatc agatgtttcc aggctccccc
     3301 aaggacctga aatgaccctg cgccttattt gaattaacca atcagcctgc ttctcgcttc
     3361 tgttcgcgcg cttctgcttc ccgagctcta taaaagagct cacaacccct cactcggcgc
     3421 gccagtcctc cgacagactg agtcgcccgg gggtctagat gtaattctcc ttggaatttg
     3481 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3541 ttcttccatt tcaggtgtcg tgaggaattt cgactgctag cgatatcaac aagtttgtac
     3601 aaaaaagttg gcatggagtc ggccgacttc tacgaggtgg agccgcggcc cccgatgagc
     3661 agtcacctcc agagcccccc gcacgcgccc agcaacgccg cctttggctt tccccggggc
     3721 gcgggccccg cgccgccccc agccccacct gccgccccgg agccgctggg cggcatctgc
     3781 gagcacgaga cgtctataga catcagcgcc tacatcgacc cggccgcctt caacgacgag
     3841 ttcctggccg acctcttcca gcacagccga cagcaggaga aggccaaggc ggcggcgggc
     3901 cccgcgggtg gcggcggtga ctttgactac ccgggagccc cggcgggccc cggcggcgcg
     3961 gtcatgtccg cgggggcgca cgggccccct cccggctacg gctgtgcggc ggccggctac
     4021 ctggacggca ggctggagcc cctgtacgag cgcgtcgggg cgcccgcgct acggccgctg
     4081 gtgatcaaac aagagccccg cgaggaggac gaggcgaagc agctggcgct ggccggcctc
     4141 ttcccctacc agccaccgcc gccaccgcca ccgccgcacc cgcacgcgtc tcccgcgcac
     4201 ctggccgccc cccacttgca gttccagatc gcgcactgcg gccagaccac catgcacctg
     4261 cagcctggcc accccacacc gccgcccacg cccgtgccca gcccgcacgc tgcgcccgcc
     4321 ttgggtgctg cgggcctgcc tggccccggg agcgcgctca agggcttggc cggtgcgcac
     4381 cccgacctcc gcacgggagg cggcggcggt ggcagcggtg ccggtgcggg caaagccaag
     4441 aagtcggtgg acaagaacag caacgagtac cgggtacggc gggaacgcaa caacatcgcg
     4501 gtgcgcaaga gccgagataa agccaaacaa cgcaacgtgg agacgcaaca gaaggtgctg
     4561 gagttgacca gtgacaatga ccgcctgcgc aagcgggtgg aacagctgag ccgtgaactg
     4621 gacacgctgc ggggcatctt ccgccagctg cctgagagct ccttggtcaa ggccatgggc
     4681 aactgcgcgt gcccaacttt cttgtacaaa gtggttgata tctgaggatc cgtttaaacg
     4741 cggccgccca gcacagtggc tcgagccgcg ggttaactgg ccagaattct cgaccttgac
     4801 tggcggcgcg accttgaggc ctgcgttcgc ctcagttgcc ccctctgtgc aatggggaga
     4861 cgcgcctcat cgcttgacaa cggccgaaga gccgccgcgc ttccgtctcc cgcgtgcgcg
     4921 cgccatgctg cccacccccg ttccgcactg accctccccc gtgccccgcg tcccgtactg
     4981 ccgccccgcc ccgagtccca tgccgcagcc accgcgacgg agcccgcagg cgggaacctg
     5041 cctccgcgcg ttagcgcgca cgcgcgcctc atgtgtcgtc cccatcagcg ccggcttccg
     5101 tctataggcc agatgcactg tcactctggc gaagtcgcag acccgattgg ccgggacgga
     5161 ggcgcgagac cgggttgcgg gcggggccga acgtggtata aaaggggcgg gaggccaggc
     5221 tcgtgccgtt ttgcagacgc caccgccgag gaaaaccgtg tactattagc cacgcgtgcc
     5281 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc
     5341 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg
     5401 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     5461 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     5521 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     5581 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     5641 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     5701 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     5761 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     5821 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     5881 tgagcgatcg ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5941 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6001 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6061 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6121 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6181 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6241 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     6301 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6361 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6421 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6481 cttaatcgcg tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     6541 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     6601 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6661 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     6721 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     6781 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     6841 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     6901 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     6961 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     7021 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7081 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7141 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgtttc atgtgagcaa
     7201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7681 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7861 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7921 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7981 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8041 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8101 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8161 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8221 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8281 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8341 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8401 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8461 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8521 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8581 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8641 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8701 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8761 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8821 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8881 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8941 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9001 acg
//
