LOCUS       sequence-215671-       12391 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentivirus for constitutive expression of ABE7.10 RA (codon 
            optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 12391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-ABERA-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..12391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..52
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             59..79
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1292..1315,1316..1318,1319..5392)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1292..5392
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5393..5440
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5456..5512
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5513..6112
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5513..5532)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6009..6029
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6128..6716
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6181..6201)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6599..6610)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6788..7021
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7099..7220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7136..7155)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7190..7209
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7260..7395
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7415..7434)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7416..7434)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7444..7461)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7444..7460)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7453..7475)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7602..8057
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7689..7708)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7899..7920
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8083..8187
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8188..8256,8257..9048)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8406..8425)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9219..9807
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9708..9727
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9961..9978
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10095..10116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10131..10136,10137..10154,10155..10161)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10169..10185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10174..10196
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10193..10209
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10193..10209
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10228..10248
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10230..10248
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10276..10502
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10503..10683
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10730..10855
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11348..11581
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11766..11810
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11994..12111
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        12180..12391
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 gtgtcgtgac gcgggatccg ccaccatgga ttacaaagac gatgacgata agatggcccc
       61 aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccagtgagg tcgaatttag
      121 tcatgagtat tggatgagac acgccctgac ccttgcaaaa cgcgcctggg atgaaaggga
      181 agtccctgtg ggggccgtcc ttgtccataa taatcgagtg attggagagg gctggaatcg
      241 ccctattgga aggcacgacc ccactgcaca cgcagagatt atggctctcc gacagggtgg
      301 actggtaatg cagaattacc ggctgatcga cgccaccctc tatgtcactc ttgaaccctg
      361 tgtaatgtgc gctggcgcca tgatccacag cagaatagga agagtcgtct tcggcgctag
      421 agatgctaaa actggagctg cagggagttt gatggatgta ctccaccacc ccgggatgaa
      481 tcatcgggtg gagataaccg aaggaatcct ggctgatgaa tgcgctgctc tgttgagcga
      541 tttctttagg atgaggaggc aggagattaa ggcacaaaag aaagctcaga gctctactga
      601 cagtgggggg agttccggtg gatctagtgg tagcgagaca cccgggactt ccgaaagtgc
      661 taccccagaa tcatccgggg ggagttcagg cggaagttct gaagtagagt tctctcacga
      721 gtattggatg cgccacgcac tgacactggc taagcgggca agggacgaac gagaagtccc
      781 agtcggggct gtcctcgtct tgaataatag agttattggg gaggggtgga accgagctat
      841 tggactgcat gacccaactg cacacgctga aattatggcc ttgagacagg gcggtctcgt
      901 aatgcagaat tatagattga tagatgctac tttgtatgtg actttcgagc catgcgtcat
      961 gtgtgccggg gcaatgatcc acagcagaat tggaagggtt gtattcggcg tccgaaacgc
     1021 taagaccggg gctgccgggt ctctcatgga cgtccttcac tatcctggta tgaatcaccg
     1081 agtggaaatt accgaaggaa tcctcgctga cgaatgcgca gccctcctct gttatttctt
     1141 tcggatgcca agacaggtct ttaatgctca gaagaaagct cagtcctcca ctgactcagg
     1201 tggctccagc ggtggaagct caggatctga gaccccagga acatctgagt cagccactcc
     1261 tgaatcctca ggtggtagct ctggggggtc tgacaagaag tacagcatcg gcctggccat
     1321 cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa
     1381 attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct
     1441 gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag
     1501 atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc
     1561 caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa
     1621 gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa
     1681 gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct
     1741 gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga
     1801 gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac
     1861 ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat
     1921 cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg
     1981 cgagaagaag aatggcctgt tcggcaacct gattgccctg agcctgggcc tgacccccaa
     2041 cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta
     2101 cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct
     2161 ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga
     2221 gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga
     2281 cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt
     2341 cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga
     2401 gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt
     2461 gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc
     2521 ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc
     2581 attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta
     2641 cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga
     2701 aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt
     2761 catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca
     2821 cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac
     2881 cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct
     2941 gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa
     3001 aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct
     3061 gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga
     3121 aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat
     3181 gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct
     3241 gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg
     3301 ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag
     3361 aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc
     3421 ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc
     3481 cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat
     3541 gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca
     3601 gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct
     3661 gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct
     3721 gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa
     3781 ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga aggacgactc
     3841 catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc
     3901 ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct
     3961 gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact
     4021 ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt
     4081 ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg
     4141 ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca
     4201 gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc
     4261 cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg
     4321 cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa
     4381 ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac
     4441 cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga
     4501 gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca
     4561 agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct
     4621 gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta
     4681 cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa
     4741 gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag
     4801 aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa
     4861 aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa
     4921 gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa
     4981 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga
     5041 taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga
     5101 gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct
     5161 gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca
     5221 cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat
     5281 cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag
     5341 catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acaagcgtcc
     5401 tgctgctact aagaaagctg gtcaagctaa gaaaaagaaa gctagcggca gcggcgccac
     5461 caacttcagc ctgctgaagc aggccggcga cgtggaggag aaccccggcc ccatgaccga
     5521 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     5581 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     5641 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     5701 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     5761 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     5821 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     5881 gttcctggcc accgtcggag tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     5941 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6001 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6061 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgtta
     6121 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6181 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6241 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6301 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6361 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6421 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6481 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     6541 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6601 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6661 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     6721 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6781 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     6841 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6901 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6961 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7021 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7081 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7141 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7201 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     7261 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7321 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7381 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     7441 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     7501 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     7561 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     7621 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     7681 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     7741 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     7801 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     7861 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7921 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     7981 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     8041 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8101 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8161 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8221 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8281 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8341 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8401 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8461 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8521 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8581 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8641 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8701 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8761 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8821 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8881 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8941 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9001 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9061 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9121 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9181 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9241 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9301 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9361 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9421 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9481 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9541 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9601 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9661 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9721 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9781 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9841 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9901 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     9961 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
    10021 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
    10081 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
    10141 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    10201 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
    10261 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
    10321 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
    10381 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
    10441 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
    10501 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    10561 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    10621 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    10681 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
    10741 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
    10801 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
    10861 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
    10921 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
    10981 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
    11041 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
    11101 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
    11161 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
    11221 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
    11281 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
    11341 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
    11401 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
    11461 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
    11521 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
    11581 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
    11641 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
    11701 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
    11761 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
    11821 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
    11881 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
    11941 caccattatc gtttcagacc cacctcccaa ccccgagggg acccagacca caattttaaa
    12001 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    12061 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    12121 tacagggaca gcagagatcc actttggcgc cggctcgagt ggctccggtg cccgtcagtg
    12181 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
    12241 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
    12301 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
    12361 ttttcgcaac gggtttgccg ccagaacaca g
//
