LOCUS       sequence-215770-       10469 bp    DNA     circular SYN 15-APR-2025
DEFINITION  TET-inducible expression of the Neurotrophin 3 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10469)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 10469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-NT3
FEATURES             Location/Qualifiers
     source          1..10469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2542
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2569..2587
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2610..2628
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2774..2792
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2854..2878
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3661..3685)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3703..4213
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4132..4150
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      4229..4238
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4235..4237,4238..4240,4241..4951)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4235..4237,4238..4240,4241..4951)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4280..4301)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4541..4560)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4888..4909
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4967..5026
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             6095..6466
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    6486..7074
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6539..6559)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6957..6968)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7077..7093)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7078..7094)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(7222..7255)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7275..7455
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7493..7512)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7558..7613
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7612..7631)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7972..7988)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7972..7988)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7985..8007)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7996..8012
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8020..8026,8027..8044,8045..8050))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8065..8086
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8145..8340
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8191..8326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8346..8480
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8383..8402)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8437..8456
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8548..8565)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8719..9307)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8799..8818)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9478..10269,10270..10338))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10101..10120
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10339..10443)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tgggtacaac tcccactaga catatcctac tctatcattg
     2581 atagagtgac ccttccatag acatatccta ctctatcatt gatagagtga cccttccact
     2641 aggttcactt ttctctatca ttgataggga gtggtaaaca attgatggac aaaatctatc
     2701 aatgatagag agtgcatgtt actagtattg ttcttctcta tcattgatag tgactttgcc
     2761 taatcactta gcaactctat cattgataga gttctagtat tagcaaggcc tttcctctat
     2821 ataaggaagt tcatttcatt tggagaagat cccccaagtt tgtacaaaaa agcaggcttc
     2881 accatgtcca tcttgtttta tgtgatattt cttgcttatc tccgtggcat ccaaggcaac
     2941 agcatggatc aaaggagttt gccggaagac tctctcaatt ccctcatcat caagctgatc
     3001 caggcggata tcttgaaaaa caagctttcc aaacagatgg tggatgttaa ggaaaattac
     3061 cagagcaccc tgcccaaagc agaggcaccc agggaaccag agcagggaga ggccaccagg
     3121 tcagagttcc agccaatgat tgcaacggac acagagctac tacggcaaca gagacgctac
     3181 aattcgcccc gggtcctgct gagtgacagc acccctttgg agccccctcc cttataccta
     3241 atggaggatt atgtgggcaa cccggtggta gccaatagaa cctcaccacg gaggaaacgc
     3301 tatgcagaac ataagagtca ccgaggagag tactcagtgt gtgacagtga gagcctgtgg
     3361 gtgaccgaca agtcctcagc cattgacatt cggggacacc aggtcacagt gctgggggag
     3421 atcaaaaccg gtaactctcc tgtgaaacaa tatttttatg aaacgagatg taaagaagcc
     3481 aggccggtca aaaacggttg cagggggatt gatgacaaac actggaactc tcagtgcaaa
     3541 acttcgcaaa cctatgtccg agcactgact tcagaaaaca acaaactcgt aggctggcgc
     3601 tggatacgaa tagacacttc ctgtgtgtgt gccttgtcga gaaaaattgg aagaacatga
     3661 acccagcttt cttgtacaaa gtggtgataa tcgaattccc acggggttgg ggttgcgcct
     3721 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     3781 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     3841 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     3901 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     3961 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4021 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt
     4081 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4141 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4201 cctctctccc cagggggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt
     4261 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     4321 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     4381 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     4441 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     4501 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     4561 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     4621 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     4681 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     4741 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     4801 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     4861 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     4921 gatcactctc ggcatggacg agctgtacaa ggctagcgga tcaggtcaat gtactaacta
     4981 cgctttgttg aagcttgccg gcgatgttga aagtaacccc ggtcctggat ccatgtccag
     5041 actggacaag agcaaagtca taaacggcgc tctggaatta ctcaatggag tcggtatcga
     5101 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
     5161 gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca
     5221 tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
     5281 caagtcattc cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
     5341 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
     5401 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
     5461 ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
     5521 cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga
     5581 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
     5641 cgaaagcggc gggccggcct cgagcgaatt ccagtacctg ccagatacag acgatcgtca
     5701 ccggattgag gagaaacgta aaaggacata tgagaccttc aagagcatca tgaagaagag
     5761 tcctttcagc ggacccaccg acccccggcc tccacctcga cgcattgctg tgccttcccg
     5821 cagctcagct tctgtcccca agccagcacc ccagccctat ccctttacgt catccctgag
     5881 caccatcaac tatgatgagt ttcccaccat ggtgtttcct tctgggcaga tcagccaggc
     5941 ctcggccttg gccccggccc ctccccaagt cctgccccag gctccagccc ctgcccctgc
     6001 tccagccatg gtatcagctc tggcccaggc cccagcccct gtcccagtcc tagccccagg
     6061 ccctcctcag gctgtggccc cacctgcccc caagcccacc caggctgggg aaggaacgct
     6121 gtcagaggcc ctgctgcagc tgcagtttga tgatgaagac ctgggggcct tgcttggcaa
     6181 cagcacagac ccagctgtgt tcacagacct ggcatccgtc gacaactccg agtttcagca
     6241 gctgctgaac cagggcatac ctgtggcccc ccacacaact gagcccatgc tgatggagta
     6301 ccctgaggct ataactcgcc tagtgacagg ggcccagagg ccccccgacc cagctcctgc
     6361 tccactgggg gccccggggc tccccaatgg cctcctttca ggagatgaag acttctcctc
     6421 cattgcggac atggacttct cagccctgct gagtcagatc agctcctaag atcaagctta
     6481 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6541 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6601 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6661 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6721 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6781 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6841 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     6901 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6961 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7021 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     7081 accgtcgacc tcgaggttcg ctttaagacc aatgacttac aaggcagctg tagatcttag
     7141 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     7201 tgggatcaat tcaccatggg aataacttcg tatagcatac attatacgaa gttatgctgc
     7261 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7321 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7381 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7441 ggaaaatctc tagcagcatc tagttgaatc gccagtgtgt cgagctagca gatctttttc
     7501 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     7561 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     7621 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     7681 caacatatgc catatgctgg ctgccatgaa caaaggtggc tataaagagg tcatcagtat
     7741 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     7801 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     7861 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     7921 cttctcttat gaagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct
     7981 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8041 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8101 actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca
     8161 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8221 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8281 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     8341 aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8401 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8461 tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8521 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8581 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8641 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8701 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8761 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8821 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8881 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     8941 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9001 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9061 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9121 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9181 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9241 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9301 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9361 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9421 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9481 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9541 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9601 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9661 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9721 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9781 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9841 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9901 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9961 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10021 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10081 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10141 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10201 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10261 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10321 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10381 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10441 gcgcacattt ccccgaaaag tgccacctg
//
