LOCUS       sequence-215792-       10726 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Ubiquitous expression of a constitutively active form of the Glis3 
            transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10726)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 10726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPG-UBI-Glis3M412
FEATURES             Location/Qualifiers
     source          1..10726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2542
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2560..3770
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3607..3626
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3777..3801
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5441..5465)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5483..5993
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5912..5930
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      6009..6018
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6015..6017,6018..6020,6021..6734)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6015..6017,6018..6020,6021..6731)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6060..6081)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6321..6340)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6668..6689
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6750..7338
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6803..6823)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7221..7232)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7479..7512)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7532..7712
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7750..7769)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7815..7870
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7869..7888)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8229..8245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8229..8245)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8242..8264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8253..8269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8277..8283,8284..8301,8302..8307))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8322..8343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8402..8597
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8448..8583
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8603..8737
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8640..8659)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8694..8713
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8805..8822)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8976..9564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9056..9075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9735..10526,10527..10595))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10358..10377
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10596..10700)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tgttaattaa gggtgcagcg gcctccgcgc cgggttttgg
     2581 cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca
     2641 ggagcgttcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact
     2701 cggccttaga accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta
     2761 gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg
     2821 attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg
     2881 gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc
     2941 gctgtgatcg tcacttggtg agttgcgggc tgctgggctg gccggggctt tcgtggccgc
     3001 cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc
     3061 gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc acaaaatggc ggctgttccc
     3121 gagtcttgaa tggaagacgc ttgtaaggcg ggctgtgagg tcgttgaaac aaggtggggg
     3181 gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta
     3241 ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact
     3301 ggagaactcg ggtttgtcgt ctggttgcgg gggcggcagt tatgcggtgc cgttgggcag
     3361 tgcacccgta cctttgggag cgcgcgcctc gtcgtgtcgt gacgtcaccc gttctgttgg
     3421 cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca
     3481 ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt
     3541 gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta
     3601 agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag
     3661 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
     3721 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac aggatcccaa
     3781 ctttgtacaa aaaagcaggc tactagtgag gaaccaattc agtcgactgg atccggtacc
     3841 gaattgatcc accatggtgt tgcagcctgg cccggtaggc caggatggcc agacagccaa
     3901 catgctcaaa acagagcgcc tggaggagtt ccccgccagt gccttggacc tgccctctgc
     3961 tctgcctctc cctcttcctc cgcctcaggg tcccccaccc ccataccatg cccatccaca
     4021 ccttcatcac ccagagctcc tgcctcacac ccaatcgctg tccctggccc agactggcct
     4081 ggaagaggat ggggagatgg aagactcagg ggggaagcac tgctgccgtt ggatagactg
     4141 cagtgccctt tatgaccagc aggaggaact ggtgaggcac atcgagaagg tccacataga
     4201 ccaacgcaag ggggaagact tcacgtgctt ctggactggc tgccctagaa gatacaagcc
     4261 tttcaacgca cggtatagac tgctgatcca catgagggtc cactctgggg agaagcccaa
     4321 caagtgtacg ttcgaaggct gcaagaaggc cttttccagg ctcgagaacc tcaagattca
     4381 cttgcggagc cacacgggtg agaagccata cttgtgccag catccgggct gccaaaaggc
     4441 cttcagcaat tccagtgacc gtgccaagca ccagaggaca catcttgaca ctaaacctta
     4501 tgcttgtcaa attccaggat gtacaaagcg ctacacagac ccaagctccc tcagaaagca
     4561 cgtgaaggca cattcttccc gagagcagca agcaaggaaa aagctacggt ctagcactga
     4621 gctccatcca gatctcctca cagactgcct cgctgtgcag cccctgcagc cagccacgtc
     4681 ccctggagat gctgctgatc ataccgtggg gcactcccca gggccggggc ctgggccagg
     4741 gcctggggct gaactctatt cagctcccat tttcgccagc aatcattcaa ctcgaagtgg
     4801 agcagctgct ggggctgggc cacccccaca tcctgtcagt cgcccttctc caggacataa
     4861 tgtacagggg agcccccaca acccctcctc ccagttacct ccactcacag ctgtggacgc
     4921 aggcgctgag aggtttgcac ctccgactcc atctcctcac cacatcagcc cgggaagagt
     4981 tccagctcct ccgtcactgc tccagagagc acaggctccc cactcccagc agccaccagg
     5041 ctcactcctg aagccctacc agccagaaac caactcttct tttcagccaa atggtatcca
     5101 tgtccacgga ttttatggcc agctgcagac attctgtccc ccgcattatc ctgattccca
     5161 aagaactgtg ccacccagcg gctcctgcag tatggtgcct tcctttgagg actgcctggt
     5221 ccccacgtcc atgggtcaag ccggttttga tgtttttcac agagccttct ctacacactc
     5281 aggcatcaca gtgtacgatt tgccttcagc ttcctcaagc ctctttgggg aatctcttcg
     5341 cagtggccct gaagacccca cgttcttgca gctcagtgct gtggaccgct gtcctagcca
     5401 gctctcctct gtgtataccg aaggctgagc ggccatctag acccagcttt cttgtacaaa
     5461 gtggtgataa tcgaattccc acggggttgg ggttgcgcct tttccaaggc agccctgggt
     5521 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     5581 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     5641 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     5701 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     5761 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     5821 cagcggggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     5881 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     5941 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc
     6001 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     6061 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     6121 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     6181 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     6241 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     6301 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     6361 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     6421 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     6481 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     6541 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     6601 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     6661 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     6721 agctgtacaa gtaaagcggc cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa
     6781 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6841 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6901 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6961 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7021 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7081 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7141 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7201 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7261 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7321 tttgggccgc ctccccgcct ggaattcccg cggttcgctt taagaccaat gacttacaag
     7381 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7441 tcccaacgaa gacaagatgg gatcaattca ccatgggaat aacttcgtat agcatacatt
     7501 atacgaagtt atgctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7561 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7621 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7681 acccttttag tcagtgtgga aaatctctag cagcatctag ttgaatcgcc agtgtgtcga
     7741 gctagcagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     7801 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     7861 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     7921 tttggtttag agtttggcaa catatgccat atgctggctg ccatgaacaa aggtggctat
     7981 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     8041 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     8101 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     8161 cagtcatagc tgtccctctt ctcttatgaa gatccctcga cctgcagccc aagcttggcg
     8221 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8281 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8341 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     8401 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     8461 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8521 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8581 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
     8641 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8701 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
     8761 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8821 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8881 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8941 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9001 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9061 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9121 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
     9181 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9241 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9301 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9361 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     9421 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9481 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9541 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9601 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9661 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9721 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9781 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9841 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9901 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9961 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10021 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10081 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10141 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10201 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10261 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10321 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10381 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10441 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10501 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10561 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10621 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10681 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//
