LOCUS       sequence-215809-        9836 bp    DNA     circular SYN 14-APR-2025
DEFINITION  TET-inducible expression of the V12-mutant form of the ki-Ras 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9836)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 9836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCLXEBR-pTF-kRasV12
FEATURES             Location/Qualifiers
     source          1..9836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2536..2554
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2577..2595
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2741..2759
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2844..2868
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2871..2903,2904..2906,2907..3437)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(3439..3463)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3500..3516
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3501..3517
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3532..3649
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3705..3916
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3935..4330
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4340..4399
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5462..5833
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    5859..6447
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5912..5932)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6330..6341)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6589..6622)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6642..6822
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6860..6879)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6925..6980
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6979..6998)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7339..7355)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7339..7355)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7352..7374)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7363..7379
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7387..7393,7394..7411,7412..7417))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7432..7453
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7512..7707
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7558..7693
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7713..7847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7750..7769)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7804..7823
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7915..7932)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8086..8674)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8166..8185)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8845..9636,9637..9705))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9468..9487
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9706..9810)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttcg gtacaactcc
     2521 cactagacat atcctactct atcattgata gagtgaccct tccatagaca tatcctactc
     2581 tatcattgat agagtgaccc ttccactagg ttcacttttc tctatcattg atagggagtg
     2641 gtaaacaatt gatggacaaa atctatcaat gatagagagt gcatgttact agtattgttc
     2701 ttctctatca ttgatagtga ctttgcctaa tcacttagca actctatcat tgatagagtt
     2761 ctagtattag caaggccttt cctctatata aggaagttca tttcatttgg agaagatccc
     2821 cggggatcct ctagagtcga atcacaagtt tgtacaaaaa agttggcacc atgactgaat
     2881 ataaacttgt ggtagttgga gctgttggcg taggcaagag tgccttgacg atacagctaa
     2941 ttcagaatca ttttgtggac gaatatgatc caacaataga ggattcctac aggaagcaag
     3001 tagtaattga tggagaaacc tgtctcttgg atattctcga cacagcaggt caagaggagt
     3061 acagtgcaat gagggaccag tacatgagga ctggggaggg ctttctttgt gtatttgcca
     3121 taaataatac taaatcattt gaagatattc accattatag agaacaaatt aaaagagtta
     3181 aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg ccttccagaa
     3241 cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct tttattgaaa
     3301 catcagcaaa gacaagacag ggtgttgatg atgccttcta tacattagtt cgagaaattc
     3361 gaaaacataa agaaaagatg agcaaagatg gtaaaaagaa gaaaaagaag tcaaagacaa
     3421 agtgtgtaat tatgtaattg ccaactttct tgtacaaagt ggtgattcga cctgcaggca
     3481 tgcaagctcg ggccccccct cgaggtcgac ggtatcgata agcttgataa cttttaaaag
     3541 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     3601 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc cgatcacgag
     3661 actagcctcg agaagcttga tcgatggctc cggtgcccgt cagtgggcag agcgcacatc
     3721 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     3781 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     3841 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     3901 tgccgccaga acacaggtgt cgtgacgcgg atccatggcc aagcctttgt ctcaagaaga
     3961 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta
     4021 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata
     4081 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc
     4141 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc
     4201 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa
     4261 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt
     4321 gtgggagggc ggatcaggtc aatgtactaa ctacgctttg ttgaagcttg ccggcgatgt
     4381 tgaaagtaac cccggtccta tgtccagact ggacaagagc aaagtcataa acggcgctct
     4441 ggaattactc aatggagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
     4501 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
     4561 cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
     4621 atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc tctcacatcg
     4681 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
     4741 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
     4801 gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc
     4861 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
     4921 tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
     4981 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccggcctcga gcgaattcca
     5041 gtacctgcca gatacagacg atcgtcaccg gattgaggag aaacgtaaaa ggacatatga
     5101 gaccttcaag agcatcatga agaagagtcc tttcagcgga cccaccgacc cccggcctcc
     5161 acctcgacgc attgctgtgc cttcccgcag ctcagcttct gtccccaagc cagcacccca
     5221 gccctatccc tttacgtcat ccctgagcac catcaactat gatgagtttc ccaccatggt
     5281 gtttccttct gggcagatca gccaggcctc ggccttggcc ccggcccctc cccaagtcct
     5341 gccccaggct ccagcccctg cccctgctcc agccatggta tcagctctgg cccaggcccc
     5401 agcccctgtc ccagtcctag ccccaggccc tcctcaggct gtggccccac ctgcccccaa
     5461 gcccacccag gctggggaag gaacgctgtc agaggccctg ctgcagctgc agtttgatga
     5521 tgaagacctg ggggccttgc ttggcaacag cacagaccca gctgtgttca cagacctggc
     5581 atccgtcgac aactccgagt ttcagcagct gctgaaccag ggcatacctg tggcccccca
     5641 cacaactgag cccatgctga tggagtaccc tgaggctata actcgcctag tgacaggggc
     5701 ccagaggccc cccgacccag ctcctgctcc actgggggcc ccggggctcc ccaatggcct
     5761 cctttcagga gatgaagact tctcctccat tgcggacatg gacttctcag ccctgctgag
     5821 tcagatcagc tcctaaagat ccgcgtcgta cggtcgacaa tcaacctctg gattacaaaa
     5881 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5941 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6001 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6061 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     6121 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     6181 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     6241 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     6301 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     6361 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     6421 ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat
     6481 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     6541 gctaattcac tcccaacgaa gacaagatgg gatcaattca ccatgggaat aacttcgtat
     6601 agcatacatt atacgaagtt atgctgcttt ttgcttgtac tgggtctctc tggttagacc
     6661 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6721 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6781 gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag ttgaatcgcc
     6841 agtgtgtcga gctagcagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     6901 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     6961 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     7021 agaatgagta tttggtttag agtttggcaa catatgccat atgctggctg ccatgaacaa
     7081 aggtggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
     7141 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
     7201 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
     7261 tgactactcc cagtcatagc tgtccctctt ctcttatgaa gatccctcga cctgcagccc
     7321 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7381 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7441 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7501 ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7561 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7621 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7681 ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt
     7741 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7801 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg
     7861 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7921 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7981 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8041 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8101 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8161 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8221 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8281 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8341 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8401 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8461 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8521 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8581 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8641 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8701 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8761 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8821 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8881 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8941 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9001 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9061 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9121 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9181 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9241 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9301 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9361 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9421 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9481 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9541 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9601 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9661 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9721 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9781 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//
