LOCUS       sequence-216839-        5243 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Luciferase reporter for the human BRN2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Zeng H, Jorapur A, Shain AH, Lang UE, Torres R, Zhang Y, McNeal AS, 
            Botton T, Lin J, Donne M, Bastian IN, Yu R, North JP, Pincus L, 
            Ruben BS, Joseph NM, Yeh I, Bastian BC, Judson RL
  TITLE     Bi-allelic Loss of CDKN2A Initiates Melanoma Invasion via BRN2 
            Activation.
  JOURNAL   Cancer Cell. 2018 Jul 9;34(1):56-68.e9. doi: 
            10.1016/j.ccell.2018.05.014.
  PUBMED    29990501
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.05"; journalName: "Cancer Cell"; date: 
            "2018-07-9- 9"; volume: "34"; issue: "1"; pages: "56-68.e9"
COMMENT     Sequence Label: pGL410-BRN2p
FEATURES             Location/Qualifiers
     source          1..5243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1095..1104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1101..2753
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2797..2918
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2808..2827)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2862..2881
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3166..3183)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3337..3925)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3417..3436)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4125..4916,4917..4985))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5090..5138
                     /note="synthetic polyadenylation signal"
     misc_feature    5152..5243
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5192..5211
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgaagc cttgatggga atgacttaca
       61 agccgcggtg cgggctgagc ctgcctctct cggggttctt tccccaaggg agtgggagac
      121 aattgggcct ggaacctaaa aggaagagag cctgtgctag ccgcggggta gaggggaagg
      181 agaagagctt tgggcagaag tgtccgcaca ccctggagca agagacactt gtgcactgaa
      241 attctaaccg tgggatgcaa ggagagttga acaggggaac aggtctggct cttggctcta
      301 ggtttaagag atacacactg tgcgtgtgtc cctaggtgaa taaatccgac tcccgctggc
      361 tcggtgcgac ccagcttttc ccttaccccc tccgtttccc catcttaaag ccctattggg
      421 aaacgaaagt ggacaccact gcctcccgcc ccccacccca atccaagtct tccaagtgtc
      481 tgtggccgat aagagcaccg ggaccgcccc cctgccggtc ttctctgcct gggagacaga
      541 tggggggcgg ggcccatccg agagagggcg gaggaggccc cgggtgagga agaagcgggg
      601 gggtgagaac tgagagaatc tgaatcggga ggcgaagggg acggggagga gggctaggag
      661 gactccgagc ccgggggagg gggagggagt agctctgcgc caatcagtgg cgccggcctg
      721 ggaggttgct agcggtatcc acgtaaatca aagggcgcgg agccaatggg agggggcgga
      781 gggggcgggg tccaggcgcg tgccgctgcg agccggcgct gccaagaggg cgggagagag
      841 ctggagagag cagggagagg ggggagcgcc gagctagtca gagagtgagc gagagcgaga
      901 aggagggaga ggaggagaaa gagagcgagg gcgggcggga ggcggcggcg gcggcagcag
      961 cagcagtaat agcaggagca gcaacagaag gcgtcggagc gggcgtcgga gctgcccgct
     1021 gtgggagaga gaggagacag aaagagcgat ctggcctcgg cggccaagct tggcaatccg
     1081 gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat
     1141 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg
     1201 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg
     1261 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata
     1321 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg
     1381 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc
     1441 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc
     1501 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata
     1561 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac
     1621 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc
     1681 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca
     1741 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg
     1801 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg
     1861 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct
     1921 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct
     1981 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca
     2041 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac
     2101 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg
     2161 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg
     2221 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg
     2281 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca
     2341 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca
     2401 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac
     2461 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg
     2521 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga
     2581 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg
     2641 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca
     2701 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg taataattct
     2761 agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat gagtttggac
     2821 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     2881 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     2941 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     3001 aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc
     3061 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     3121 atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc
     3181 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3301 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3361 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3781 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3841 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3901 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3961 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4021 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4081 aacttggtct gacagcggcc gcaaatgcta aaccactgca gtggttacca gtgcttgatc
     4141 agtgaggcac cgatctcagc gatctgccta tttcgttcgt ccatagtggc ctgactcccc
     4201 gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag gccccagcgc agcaatgatg
     4261 ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg
     4321 gccgagcgaa gaagtggtcc tgctactttg tccgcctcca tccagtctat gagctgctgt
     4381 cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc gaagagttgt ggccattgct
     4441 actggcatcg tggtatcacg ctcgtcgttc ggtatggctt cgttcaactc tggttcccag
     4501 cggtcaagcc gggtcacatg atcacccata ttatgaagaa atgcagtcag ctccttaggg
     4561 cctccgatcg ttgtcagaag taagttggcc gcggtgttgt cgctcatggt aatggcagca
     4621 ctacacaatt ctcttaccgt catgccatcc gtaagatgct tttccgtgac cggcgagtac
     4681 tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa gctgctcttg cccggcgtct
     4741 atacgggaca acaccgcgcc acatagcagt actttgaaag tgctcatcat cgggaatcgt
     4801 tcttcggggc ggaaagactc aaggatcttg ccgctattga gatccagttc gatatagccc
     4861 actcttgcac ccagttgatc ttcagcatct tttactttca ccagcgtttc ggggtgtgca
     4921 aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg
     4981 ctcatactcg tcctttttca atattattga agcatttatc agggttacta gtacgtctct
     5041 caaggataag taagtaatat taaggtacgg gaggtattgg acaggccgca ataaaatatc
     5101 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
     5161 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
     5221 tgcaggtgcc agaacatttc tct
//
