LOCUS       sequence-217047-       11180 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Donor vector for targeting H2B-miRFP703 into Rosa26 locus to 
            generate reporter mice or cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11180)
  AUTHORS   Gu B, Posfai E, Rossant J
  TITLE     Efficient generation of targeted large insertions by microinjection 
            into two-cell-stage mouse embryos.
  JOURNAL   Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166.
  PUBMED    29889212
REFERENCE   2  (bases 1 to 11180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166."
COMMENT     Sequence Label: pR26-CAG-H2B-miRFP703
FEATURES             Location/Qualifiers
     source          1..11180
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(25..58)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             138..515
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      528..537
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             534..1481
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 703 nm (Shcherbakova et al., 2016)"
                     /label=miRFP703
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     primer_bind     complement(1499..1515)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1499..1515)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1529..1546)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1535..1759
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    1825..1959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2032..6372
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(6422..6442)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6422..6440)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6461..6477)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6461..6477)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6474..6496)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6485..6501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6509..6515,6516..6533,6534..6539))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6554..6575
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6692..6709)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6863..7451)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6943..6962)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7622..8413,8414..8482))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8245..8264
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8483..8587)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8614..9069
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8701..8720)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8911..8932
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9195..9217
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9209..9226
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9210..9226
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9236..9254
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    9293..10375
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(10398..10463,10464..10501)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     intron          10745..10810
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    10857..10991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    11123..11170
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 cctgtaacaa ccggtacagt tcgaataact tcgtatagca tacattatac gaagttatac
       61 cgggccacca tagtcgcgag tagcttgggc cagctaggcc ttgaccaaag ttcctctgga
      121 attgcgatcg cacgcgtatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct
      181 ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca
      241 aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca
      301 tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg
      361 caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga
      421 tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg
      481 gtactaaggc catcaccaag tacaccagcg ctaaggatcc accggtcgcc accatggtag
      541 caggtcatgc ctctggcagc cccgcattcg ggaccgcctc tcattcgaat tgcgaacatg
      601 aagagatcca cctcgccggc tcgatccagc cgcatggcgc gcttctggtc gtcagcgaac
      661 atgatcatcg cgtcatccag gccagcgcca acgccgcgga atttctgaat ctcggaagcg
      721 tactcggcgt tccgctcgcc gagatcgacg gcgatctgtt gatcaagatc ctgccgcatc
      781 tcgatcccac cgccgaaggc atgccggtcg cggtgcgctg ccggatcggc aatccctcta
      841 cggagtactg cggtctgatg catcggcctc cggaaggcgg gctgatcatc gaactcgaac
      901 gtgccggccc gtcgatcgat ctgtcaggca cgctggcgcc ggcgctggag cggatccgca
      961 cggcgggttc actgcgcgcg ctgtgcgatg acaccgtgct gctgtttcag cagtgcaccg
     1021 gctacgaccg ggtgatggtg tatcgtttcg atgagcaagg ccacggcctg gtattctccg
     1081 agtgccatgt gcctgggctc gaatcctatt tcggcaaccg ctatccgtcg tcgctggtcc
     1141 cgcagatggc gcggcagctg tacgtgcggc agcgcgtccg cgtgctggtc gacgtcacct
     1201 atcagccggt gccgctggag ccgcggctgt cgccgctgac cgggcgcgat ctcgacatgt
     1261 cgggctgctt cctgcgctcg atgtcgccga tccatctgca gttcctgaag gacatgggcg
     1321 tgcgcgccac cctggcggtg tcgctggtgg tcggcggcaa gctgtggggc ctggttgtct
     1381 gtcaccatta tctgccgcgc ttcatccgtt tcgagctgcg ggcgatctgc aaacggctcg
     1441 ccgaaaggat cgcgacgcgg atcaccgcgc ttgagagcta aacgcgtaaa tgattgcaga
     1501 tccactagtt ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     1561 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     1621 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     1681 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     1741 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gagcctctag
     1801 aactatagtg agtcgtatta cgtagatcca gacatgataa gatacattga tgagtttgga
     1861 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     1921 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     1981 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc cctagaagat
     2041 gggcgggagt cttctgggca ggcttaaagg ctaacctggt gtgtgggcgt tgtcctgcag
     2101 gggaattgaa caggtgtaaa attggaggga caagacttcc cacagatttt cggttttgtc
     2161 gggaagtttt ttaatagggg caaataagga aaatgggagg ataggtagtc atctggggtt
     2221 ttatgcagca aaactacagg ttattattgc ttgtgatccg cctcggagta ttttccatcg
     2281 aggtagatta aagacatgct cacccgagtt ttatactctc ctgcttgaga tccttactac
     2341 agtatgaaat tacagtgtcg cgagttagac tatgtaagca gaattttaat catttttaaa
     2401 gagcccagta cttcatatcc atttctcccg ctccttctgc agccttatca aaaggtattt
     2461 tagaacactc attttagccc cattttcatt tattatactg gcttatccaa cccctagaca
     2521 gagcattggc attttccctt tcctgatctt agaagtctga tgactcatga aaccagacag
     2581 attagttaca tacaccacaa atcgaggctg tagctggggc ctcaacactg cagttctttt
     2641 ataactcctt agtacacttt ttgttgatcc tttgccttga tccttaattt tcagtgtcta
     2701 tcacctctcc cgtcaggtgg tgttccacat ttgggcctat tctcagtcca gggagtttta
     2761 caacaataga tgtattgaga atccaaccta aagcttaact ttccactccc atgaatgcct
     2821 ctctcctttt tctccattta taaactgagc tattaaccat taatggtttc caggtggatg
     2881 tctcctcccc caatattacc tgatgtatct tacatattgc caggctgata ttttaagaca
     2941 ttaaaaggta tatttcatta ttgagccaca tggtattgat tactgcttac taaaattttg
     3001 tcattgtaca catctgtaaa aggtggttcc ttttggaatg caaagttcag gtgtttgttg
     3061 tctttcctga cctaaggtct tgtgagcttg tattttttct atttaagcag tgctttctct
     3121 tggactggct tgactcatgg cattctacac gttattgctg gtctaaatgt gattttgcca
     3181 agcttcttca ggacctataa ttttgcttga cttgtagcca aacacaagta aaatgattaa
     3241 gcaacaaatg tatttgtgaa gcttggtttt taggttgttg tgttgtgtgt gcttgtgctc
     3301 tataataata ctatccaggg gctggagagg tggctcggag ttcaagagca cagactgctc
     3361 ttccagaagt cctgagttca attcccagca accacatggt ggctcacaac catctgtaat
     3421 gggatctgat gccctcttct ggtgtgtctg aagaccacaa gtgtattcac attaaataaa
     3481 taaatcctcc ttcttcttct tttttttttt tttaaagaga atactgtctc cagtagaatt
     3541 tactgaagta atgaaatact ttgtgtttgt tccaatatgg tagccaataa tcaaattact
     3601 ctttaagcac tggaaatgtt accaaggaac taatttttat ttgaagtgta actgtggaca
     3661 gaggagccat aactgcagac ttgtgggata cagaagacca atgcagactt taatgtcttt
     3721 tctcttacac taagcaataa agaaataaaa attgaacttc tagtatccta tttgtttaaa
     3781 ctgctagctt tacttaactt ttgtgcttca tctatacaaa gctgaaagct aagtctgcag
     3841 ccattactaa acatgaaagc aagtaatgat aattttggat ttcaaaaatg tagggccaga
     3901 gtttagccag ccagtggtgg tgcttgcctt tatgccttta atcccagcac tctggaggca
     3961 gagacaggca gatctctgag tttgagccca gcctggtcta cacatcaagt tctatctagg
     4021 atagccagga atacacacag aaaccctgtt ggggaggggg gctctgagat ttcataaaat
     4081 tataattgaa gcattcccta atgagccact atggatgtgg ctaaatccgt ctacctttct
     4141 gatgagattt gggtattatt ttttctgtct ctgctgttgg ttgggtcttt tgacactgtg
     4201 ggctttcttt aaagcctcct tcctgccatg tggtctcttg tttgctacta acttcccatg
     4261 gcttaaatgg catggctttt tgccttctaa gggcagctgc tgagatttgc agcctgattt
     4321 ccagggtggg gttgggaaat ctttcaaaca ctaaaattgt cctttaattt tttttttaaa
     4381 aaatgggtta tataataaac ctcataaaat agttatgagg agtgaggtgg actaatatta
     4441 aatgagtccc tcccctataa aagagctatt aaggcttttt gtcttatact taactttttt
     4501 tttaaatgtg gtatctttag aaccaagggt cttagagttt tagtatacag aaactgttgc
     4561 atcgcttaat cagattttct agtttcaaat ccagagaatc caaattcttc acagccaaag
     4621 tcaaattaag aatttctgac ttttaatgtt aatttgctta ctgtgaatat aaaaatgata
     4681 gcttttcctg aggcagggtc tcactatgta tctctgcctg atctgcaaca agatatgtag
     4741 actaaagttc tgcctgcttt tgtctcctga atactaaggt taaaatgtag taatactttt
     4801 ggaacttgca ggtcagattc ttttataggg gacacactaa gggagcttgg gtgatagttg
     4861 gtaaaatgtg tttcaagtga tgaaaacttg aattattatc accgcaacct actttttaaa
     4921 aaaaaaagcc aggcctgtta gagcatgctt aagggatccc taggacttgc tgagcacaca
     4981 agagtagtta cttggcaggc tcctggtgag agcatatttc aaaaaacaag gcagacaacc
     5041 aagaaactac agttaaggtt acctgtcttt aaaccatctg catatacaca gggatattaa
     5101 aatattccaa ataatatttc attcaagttt tcccccatca aattgggaca tggatttctc
     5161 cggtgaatag gcagagttgg aaactaaaca aatgttggtt ttgtgatttg tgaaattgtt
     5221 ttcaagtgat agttaaagcc catgagatac agaacaaagc tgctatttcg aggtctcttg
     5281 gtttatactc agaagcactt ctttgggttt ccctgcacta tcctgatcat gtgctaggcc
     5341 taccttaggc tgattgttgt tcaaataaac ttaagtttcc tgtcaggtga tgtcatatga
     5401 tttcatatat caaggcaaaa catgttatat atgttaaaca tttgtactta atgtgaaagt
     5461 taggtctttg tgggtttgat ttttaatttt caaaacctga gctaaataag tcatttttac
     5521 atgtcttaca tttggtggaa ttgtataatt gtggtttgca ggcaagactc tctgacctag
     5581 taaccctacc tatagagcac tttgctgggt cacaagtcta ggagtcaagc atttcacctt
     5641 gaagttgaga cgttttgtta gtgtatacta gtttatatgt tggaggacat gtttatccag
     5701 aagatattca ggactatttt tgactgggct aaggaattga ttctgattag cactgttagt
     5761 gagcattgag tggcctttag gcttgaattg gagtcacttg tatatctcaa ataatgctgg
     5821 ccttttttaa aaagcccttg ttctttatca ccctgttttc tacataattt ttgttcaaag
     5881 aaatacttgt ttggatctcc ttttgacaac aatagcatgt tttcaagcca tatttttttt
     5941 cctttttttt tttttttttg gtttttcgag acagggtttc tctgtatagc cctggctgtc
     6001 ctggaactca ctttgtagac caggctggcc tcgaactcag aaatccgcct gcctctgcct
     6061 cctgagtgcc gggattaaag gcgtgcacca ccacgcctgg ctaagttgga tattttgtta
     6121 tataactata accaatacta actccactgg gtggattttt aattcagtca gtagtcttaa
     6181 gtggtcttta ttggcccttc attaaaatct actgttcact ctaacagagg ctgttggtac
     6241 tagtggcact taagcaactt cctacggata tactagcaga ttaagggtca gggatagaaa
     6301 ctagtctagc gttttgtata cctaccagct ttatactacc ttgttctgat agaaatattt
     6361 caggacatct agcttatcga taccgtcgac ggtatcgata agcttgatcc agcttttgtt
     6421 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6481 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     6541 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6601 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     6661 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6721 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6781 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6841 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6901 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6961 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7021 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7081 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7141 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7201 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7261 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7321 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7381 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7441 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7501 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7561 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7621 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7681 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7741 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7801 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7861 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7921 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7981 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8041 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8101 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8161 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8221 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8281 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8341 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8401 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8461 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8521 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8581 ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg
     8641 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     8701 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     8761 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     8821 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     8881 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     8941 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     9001 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     9061 ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     9121 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     9181 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
     9241 cgactcacta tagggcgaat tggagctcca ccgcattacc ctgttatccc tagcggcagg
     9301 ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt gacgctggaa
     9361 ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga gggggaggga
     9421 gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg ggggcagcgg
     9481 aggagcgctt ccggccgacg tctcgtcgct gattggcttc ttttcctccc gccgtgtgtg
     9541 aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg gcagccggag
     9601 tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta ggtggggtga
     9661 ggcgagctgg acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg gagggagggt
     9721 cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg ggggagtcgt
     9781 tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca gaaaactggc
     9841 ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc gggggcggcg
     9901 aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt ctggccgcgc
     9961 gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag tagggctgag
    10021 cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag gggcgtgctg
    10081 agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc tcagttgggc
    10141 tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt atcagtaagg
    10201 gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg ggaggggagt
    10261 gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac cgccctgggc
    10321 ctgggagaat cccttccccc tcttccctcg tgatctgcaa ctccagtctt tctaggcgcg
    10381 cccgggctgc agatctgtag ggcgcagtag tccagggttt ccttgatgat gtcatactta
    10441 tcctgtccct tttttttcca cagctcgcgg ttgaggacaa actcttcgcg gtctttccag
    10501 tggggatcga cggtatcgat aagctggccg ctctagtggc cgtacgggcc cacctgccgg
    10561 gccacttaat taaatttaaa tcacaattcc agctgagcgc cggtcgctac cattaccagt
    10621 tggtctggtg tcaaaaataa taataaccgg gcagggggga tctgcatgga tctttgtgaa
    10681 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
    10741 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
    10801 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgccagatc
    10861 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
    10921 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
    10981 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
    11041 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatctgc
    11101 ggccgtgcta gcgcttaagc ttgaagttcc tattccgaag ttcctattct ctagaaagta
    11161 taggaacttc ggcgcgccgg
//
