LOCUS       sequence-217259-        9042 bp    DNA     circular SYN 15-APR-2025
DEFINITION  fluorescent, functional, glutamate receptor subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Sheridan DL, Robert A, Cho CH, Howe JR, Hughes TE
  TITLE     Regions of alpha-amino-5-methyl-3-hydroxy-4-isoxazole propionic acid
            receptor subunits that are permissive for the insertion of green 
            fluorescent protein.
  JOURNAL   Neuroscience. 2006 Aug 25;141(2):837-49. Epub 2006 Jun 12.
  PUBMED    16765522
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience."; date: "2006-08-25"; volume: "141(2)"; pages: 
            "837-49. Epub 2006 Jun 12"
COMMENT     Sequence Label: pR2Q105y
FEATURES             Location/Qualifiers
     source          1..9042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     936..952
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     936..952
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    2222..2240
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     CDS             2249..2959
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(2288..2309)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2549..2568)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2896..2917
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2977..2995
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     regulatory      5342..5351
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5355..5489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5392..5411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5446..5465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        5558..5915
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5558..5749
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5581..5601)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5766..5901
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5935..5952)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5935..5951)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5944..5966)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6164..6619
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6251..6270)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6461..6482
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6636..6655)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6755..6777
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6815..6833)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6901..7005
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7006..7074,7075..7866)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7224..7243)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8037..8625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8526..8545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8779..8796
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8913..8934
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8949..8954,8955..8972,8973..8979)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8987..9003
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8992..9014
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9011..9027
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9011..9027
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attccccggg gatcctctag aactagtgga tcccccgggc
      961 tgcaggaatt cggcacgagg ccagtcctcg ggacttcggg agcagggaca ggacgcaagg
     1021 catcaacagc caccagctac aactgggaaa taaggattct tctgccttca cttcgtgttt
     1081 ttagcagctc cttgctaaat atcgacctca caatgcagag gatctaattt gctgaggaaa
     1141 acagtcaaag aaggaagagg aagaaaggga aacgagggga tattttgtgg atgctctact
     1201 tttcttggaa atgcaaaaga ttatgcatat ttctgtcctc ctttctcctg ttttatgggg
     1261 actgattttt ggtgtctctt ctaacagcat acagataggg gggctatttc caaggggcgc
     1321 tgatcaagaa tacagtgcat ttcgggtagg gatggttcag ttttccactt cggagttcag
     1381 actgacaccc catatcgaca atttggaggt agccaacagt ttcgcagtca ccaatgcttt
     1441 ctgctcccag ttttcaagag gagtctacgc aatttttgga ttttatgaca agaagtctgt
     1501 aaataccatc acatcattct gtgggacact ccatgtgtcc ttcatcacac ctagcttccc
     1561 aacagatggc acacatccat ttgtcatcca gatgcgacct gacctcaaag gagcactcct
     1621 tagcttgatt gagtactacc aatgggacaa gttcgcatac ctctatgaca gtgacagagg
     1681 cttatcaaca ctgcaagctg ttctggattc tgctgcagag aagaagtggc aggtgactgc
     1741 tatcaatgtg gggaacatca acaatgacaa gaaagatgag acctacagat cgctctttca
     1801 agatctggag ttaaaaaaag aacggcgtgt aatcctggac tgtgaaaggg ataaagtaaa
     1861 tgacattgtg gaccaggtta ttaccattgg aaaacatgtt aaagggtacc attatatcat
     1921 tgcaaatctg ggattcactg atggggacct gctgaaaatt cagtttggag gagcaaatgt
     1981 ctctggattt cagattgtag actacgatga ttccctggtg tctaaattta tagaaagatg
     2041 gtcaacactg gaagagaaag aataccctgg agcacacaca gcgacaatta agtatacttc
     2101 ggccctgacg tatgatgctg tccaagtgat gactgaagca ttccgtaacc ttcggaagca
     2161 gaggattgaa atatcccgga gaggaaatgc aggggattgt ttggccaacc cagctgtgcc
     2221 cctgtctctt atacacatct ggcgcgccag caagggcgag gagctgttca ccggggtggt
     2281 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2341 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     2401 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc
     2461 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     2521 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     2581 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2641 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     2701 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     2761 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2821 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     2881 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2941 catggacgag ctgtacaagg gtgggcgcgc ccgggcagat gtgtataaga gacaggctgt
     3001 gccctgggga caaggggtcg aaatagaaag ggccctcaag caggttcaag ttgaaggcct
     3061 ctctggaaat ataaagtttg accagaatgg aaaacgaata aactacacaa ttaacatcat
     3121 ggagctcaaa acaaatggac cccggaagat tgggtactgg agtgaagtgg ataaaatggt
     3181 tgtcacccta actgagctcc catcaggaaa tgacacgtct gggcttgaaa acaagactgt
     3241 ggtggtcacc acaatattgg aatctccata tgttatgatg aagaaaaatc atgaaatgct
     3301 tgaagggaat gagcgttacg agggctactg tgttgactta gctgcagaaa ttgccaaaca
     3361 ctgtgggttc aagtacaagc tgactattgt tggggatggc aagtatgggg ccagggatgc
     3421 cgacaccaaa atttggaatg gtatggttgg agagcttgtc tacgggaaag ctgacattgc
     3481 aattgctcca ttaactatca ctctcgtgag agaagaggtg attgacttct ccaagccctt
     3541 catgagtctt ggaatctcta tcatgatcaa gaagcctcag aagtccaaac caggagtgtt
     3601 ttcctttctt gatcctttag cctatgagat ctggatgtgc attgtgtttg cctacattgg
     3661 ggtcagtgta gttttattcc tggtcagcag atttagcccc tacgagtggc acactgagga
     3721 atttgaagat ggaagagaaa cacaaagtag tgaatcaact aatgaatttg ggatttttaa
     3781 tagtctctgg ttttccttgg gtgcctttat gcagcaggga tgcgatattt cgccaagatc
     3841 cctctctggg cgcattgttg gaggtgtgtg gtggttcttt accctgatca taatctcctc
     3901 ctacacggct aacttagctg ccttcctgac tgtagagagg atggtgtctc ccatcgaaag
     3961 tgctgaggat ctgtctaagc aaacagaaat tgcttatgga acattagact ctggctccac
     4021 taaagagttt ttcaggagat ctaaaatcgc agtgtttgat aaaatgtgga cttatatgag
     4081 gagtgcagag ccctctgtgt ttgtgaggac taccgcagaa ggagtagcca gagtccggaa
     4141 atccaaagga aagtatgcct acttgctgga gtccacaatg aacgagtaca tcgagcagag
     4201 gaagccttgt gacaccatga aagtgggagg aaacttggat tccaaaggct acggcatcgc
     4261 cacacctaaa ggatcctcat taggaacccc agtaaatctt gcagtattga aactcagtga
     4321 gcaaggcgtc ttagacaagc tgaaaaacaa atggtggtac gataaaggtg aatgtggagc
     4381 caaggactcg ggaagtaagg aaaagaccag tgccctcagt ctgagcaacg ttgctggagt
     4441 attctacatc cttgtcgggg gccttggttt ggcaatgctg gtggctttga ttgagttctg
     4501 ttacaagtca agggccgagg cgaaacgaat gaaggtggca aagaatccac agaatattaa
     4561 cccatcttcc tcgcagaatt cccagaattt tgcaacttat aaggaaggtt acaacgtata
     4621 tggcatcgag agtgttaaaa tttaggggat gaccttgagt gatgtcatga ggagcaaggc
     4681 aaggctgtca attacaggaa gtactggaga aaatggacgt gttatgactc cagaatttcc
     4741 caaagcagtg catgctgtcc cttacgtgag tcctggcatg ggaatgaatg tcagtgtgac
     4801 tgatctctcg tgattgatag gaaccttctg agtgccttac acaatggttt ccttgtgtgt
     4861 ttattgtcaa agtggtgaga ggcatccgat atcttgaagg cttttctttc agccaagaat
     4921 tcttaactat gtggagttca ccttgaattg taaggaaaga taaattacaa acagagcatc
     4981 attttctact ccgatatcag aggaagcgtg gtggacatgc acagctaaca tggaaatact
     5041 atcatttaac tgaagtcttt gtacagacaa caaacccgtt tccgcagcca ctattgttag
     5101 tctcttgatt cataatgact taagcacact tgacatcaac tgcatcaaga tgtgacctgt
     5161 tttataaata aaaaggaaaa aaacatttaa aataaaaaaa tatttttagg tattttcaca
     5221 gacaaactgg cttttaaata aatttgcttc cgtactggtt ggttaagaca aatacaaaaa
     5281 caaaaacaaa acaaaacaaa aaccaaaaaa aaaaaaaaaa aactcgacct gcagaagctt
     5341 ggccgccatg gcccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5401 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5461 tcatcaatgt atcttatcat gtctggatcg ggaattaatt cggcgcagca ccatggcctg
     5521 aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt
     5581 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5641 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5701 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     5761 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5821 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     5881 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct gttaacagct tggcactggc
     5941 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6001 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6061 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     6121 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     6181 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6241 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6301 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6361 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6421 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6481 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     6541 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6601 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     6661 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     6721 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     6781 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     6841 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     6901 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6961 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     7021 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7081 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     7141 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7201 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     7261 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7321 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7381 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7441 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7501 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     7561 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7621 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7681 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7741 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7801 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7861 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7921 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7981 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8041 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8101 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8161 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8221 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8281 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8341 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8401 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8461 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8521 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8581 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8641 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8701 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8761 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8821 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     8881 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     8941 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     9001 caatttcaca caggaaacag ctatgacatg attacgaatt aa
//
