LOCUS       sequence-217300-        5336 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses aa270 to C-terminus of VCre recombinase fused to FKBP CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2588_pCAG-PV1-FKBP-L1-VCre-270C-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5336
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             686..1006
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     regulatory      1272..1281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1382..1402
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1412..1636
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1876..1895)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1941..1996
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1995..2014)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2357..2373)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2357..2373)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2370..2392)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2381..2397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2405..2411,2412..2429,2430..2435))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2450..2471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2556..2690
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2710..2844
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2747..2766)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2801..2820
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2912..2929)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3083..3671)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3163..3182)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3842..4633,4634..4702))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4465..4484
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4703..4807)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4838..5217
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgt ctagaggagt gcaggtggaa accatctccc caggagacgg
      721 gcgcaccttc cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga
      781 tggaaagaaa tttgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa
      841 gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc
      901 caaactgact atatctccag attatgccta tggtgccact gggcacccag gcatcatccc
      961 accacatgcc actctcgtct tcgatgtgga gcttctaaaa ctggaatccg gagggtctgg
     1021 ctccggatca agtggtggca gcggtaccaa gcccaagaag gacaagcaga ccggcgaggt
     1081 gctgcacaag cccatcacca ccaagacagt ggaaggcgtg ttctacagcg cctgggagac
     1141 actggacctg ggcagacagg gcgtgaagcc tttcacagcc cacagcgcca gagtgggagc
     1201 cgctcaggac ctgctgaaga agggctacaa taccctgcag atccagcagt ccggccggtg
     1261 gtctagcgga gccatggtgg ccagatacgg cagagccatc ctggctaggg atggcgctat
     1321 ggcccacagc agagtgaaaa ccagatccgc ccccatgcag tggggcaagg acgagaagga
     1381 ccccaagaaa aagcggaagg tgtgagaatt cctgtgcctt ctagttgcca gccatctgtt
     1441 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1501 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1561 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     1621 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     1681 cacgcgccct gtagcgtgta ccgctgggag ttcgtagacg gaaacaaacg cagaatccaa
     1741 gcgcggccgc ccaggataca tagattacca caactccgag cccttccacc gctagcgcac
     1801 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     1861 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     1921 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     1981 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     2041 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     2101 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     2161 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     2221 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     2281 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     2341 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2401 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2461 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2521 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccagttc
     2581 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     2641 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     2701 caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2761 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2821 aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag
     2881 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2941 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3001 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3061 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3121 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3181 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3241 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3301 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3361 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3421 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3481 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3541 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3601 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3661 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3721 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3781 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3841 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3901 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3961 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4021 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4081 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4141 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4201 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4261 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4321 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4381 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4441 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4501 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4561 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4621 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4681 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4741 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4801 ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt gactagttat
     4861 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     4921 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     4981 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     5041 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     5101 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     5161 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
     5221 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
     5281 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggg
//
