LOCUS       sequence-217345-        4607 bp    DNA     circular SYN 15-APR-2025
DEFINITION  firefly luciferase (Fluc) reporter containing the 5'-UTR of TAK1 
            mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ku YC, Lai MH, Lo CC, Cheng YC, Qiu JT, Tarn WY, Lai MC
  TITLE     DDX3 Participates in Translational Control of Inflammation Induced 
            by Infections and Injuries.
  JOURNAL   Mol Cell Biol. 2018 Dec 11;39(1). pii: MCB.00285-18. doi: 
            10.1128/MCB.00285-18. Print 2019 Jan 1.
  PUBMED    30373933
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2018-12-11"; volume: "39(1). pii: MCB.00285-18. doi";
            pages: " 10.1128/MCB.00285-18. Print 2019 Jan 1"
COMMENT     Sequence Label: pFL-SV40-TAK1 5'-UTR
FEATURES             Location/Qualifiers
     source          1..4607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(85..105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      873..882
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             879..2531
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(945..963)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2562..2683
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2573..2592)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2627..2646
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2816..2920
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2921..2989,2990..3781)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3139..3158)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3952..4540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4441..4460
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgtcct ctctcgcggt atcatccggt tgctgaggcc ctgtaataaa ggtctcgcga
      481 aatttgttct agaggtccaa gtttgcttct tagcttactc caccccaccc ccaacctgtc
      541 cctccttttc tttccaagtc acaaaattct cccctcccct accccggagt ttacggccct
      601 cctcctgttt ccgatttcag cccggaaccg gaagtgtagt gggcggggcc cgtcggcgga
      661 aaacgcagcg gagccagagc cggacacggc tgtggccgct gcctctaccc ccgccacgga
      721 tcgccgggta gtaggactgc gcggctccag gctgagggtc ggtccggagg cgggtgggcg
      781 cgggtctcac ccggattgtc cgggtggcac cgttcccggc cccaccgggc gccgcgaggg
      841 atcaagcttg gcattccggt actgttggta aagccaccat ggaagacgcc aaaaacataa
      901 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata
      961 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
     1021 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga
     1081 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
     1141 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
     1201 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg
     1261 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa
     1321 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
     1381 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata
     1441 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg
     1501 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg
     1561 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
     1621 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
     1681 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg
     1741 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
     1801 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg
     1861 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag
     1921 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
     1981 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag
     2041 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga
     2101 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
     2161 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc
     2221 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag
     2281 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
     2341 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
     2401 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
     2461 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga
     2521 tcgccgtgta attctagagc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     2581 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2641 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     2701 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     2761 ctacaaatgt ggtaaaatcg ataaggatcc aggtggcact tttcggggaa atgtgcgcgg
     2821 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2881 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2941 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3001 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3061 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3121 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3181 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3241 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3301 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3361 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3421 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3481 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3541 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3601 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     3661 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     3721 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     3781 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3841 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3901 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3961 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4021 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4081 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4141 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4201 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4261 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4321 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4381 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4441 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4501 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4561 tttacggttc ctggcctttt gctggccttt tgctcacatg gctcgac
//
