LOCUS       sequence-217460-        1779 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses enhanced sodium pump for light-driven extrusion of sodium;
            activation with green light; codon-optimized for human/mouse.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Grimm C, Silapetere A, Vogt A, Bernal Sierra YA, Hegemann P
  TITLE     Electrical properties, substrate specificity and optogenetic 
            potential of the engineered light-driven sodium pump eKR2.
  JOURNAL   Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.1038/s41598-018-27690-w.
  PUBMED    29915394
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-06-18"; volume: "8(1):9316. doi"; pages: " 
            10.1038/s41598-018-27690-w"
COMMENT     Sequence Label: pEYFP-N1-eKR2
FEATURES             Location/Qualifiers
     source          1..1779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1042..1044,1045..1755)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1084..1105)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1345..1364)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1692..1713
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggactatg ggggagcact gtccgccgtc gggctgtttc agacttccta caccctggag
       61 aacaacggga gcgtcatttg cattcctaac aatggccagt gcttctgtct ggcctggctg
      121 aagagcaacg ggacccagga actgggcaat gccaactttg agaatttcat cggcgccacc
      181 gagggcttta gcgagatcgc ctatcagttc acctcccaca tcctgacact gggctatgcc
      241 gtgatgctgg ccggcctgct gtactttatc ctgacaatca agaacgtgga caagaagttc
      301 cagatgtcta atatcctgag cgccgtggtc atggtgagcg ccttcctgct gctgtatgcc
      361 caggcccaga actggaccag ctcctttaca ttcaatgagg aagtgggcag gtactttctg
      421 gacccatccg gcgatctgtt caacaatggc tacagatatc tgaactggct gatcgatgtg
      481 cccatgctgc tgtttcagat cctgttcgtg gtgtccctga ccacatctaa gttctctagc
      541 gtgcggaacc agttttggtt ctccggcgcc atgatgatca tcaccggcta tatcggccag
      601 ttttacgagg tgtctaatct gacagccttc ctggtgtggg gcgccatctc ctctgccttc
      661 tttttccaca tcctgtgggt catgaagaaa gtgatcaacg agggcaagga gggaatctct
      721 ccagcaggcc agaagatcct gagcaacatc tggattctgt tcctgatcag ctggaccctg
      781 taccccggcg cctatctgat gccttacctg acaggcgtgg acggcttcct gtactccgag
      841 gatggcgtga tggcaaggca gctggtgtat accatcgccg acgtgagctc caaagtgatc
      901 tacggcgtgc tgctgggcaa tctggcaatc accctgtcaa aaaacaagga actggtcgag
      961 gcaaatagcg cagcaccggt aaagagcagg atcaccagcg agggcgagta catccccctg
     1021 gaccagatcg acatcaacgt ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1081 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1141 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1201 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc
     1261 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1321 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1381 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1441 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1501 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1561 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1621 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     1681 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1741 gacgagctgt acaagttctg ctacgagaac gaggtgtga
//
