LOCUS       sequence-217873-       13389 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13389)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU34.2-2.2
FEATURES             Location/Qualifiers
     source          1..13389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1497..1796
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4345..4565
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4566..5369
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(9536..9556)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9536..9554)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9893..10481)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9973..9992)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10652..11443,11444..11512))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11275..11294
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11513..11617)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11644..12986
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12607..12654)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     13027..13045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13364..13380)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13364..13380)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atgacggacg ataaaggggg ctacaagcag atcaacaagt
      541 cgctcaacat ctgcgctttc gaggactatt tgaaaggcca gaccgcgaac ctcgccccta
      601 tacctgatgt ggagcaagtg acagaaaggg ttttacgagt gcggggtcaa aatccgggga
      661 agttcacctt ccaaggcacc aataccttct tagttggaac aggagcctct cgcatattga
      721 tcgatacatc cggaggagag cctgagtatg cgaagctgct tgcatcaacc cttgagtctc
      781 ggaacattag catcaagtat gttcttgtaa cacattggca tggagaccac tccgggggtg
      841 taccggacct tatcagaatg tatccgcact tgaaagatca catctacaag aacgatcccg
      901 atccgggcca acaagatatc cacgaagggc aagagttttg tgtcgagggg gctactgttg
      961 tagcactgca ctgcccgggg cattcagaag atcacatgtg tttcatgctc aaggaagaga
     1021 actccatgtt cactggagac aacattctcg gtaccgggac gagcgcggtc gaagacttgg
     1081 gcatattcat gtccagtctg cacaagatga tggatcagaa atgtcgagct ggccaccccg
     1141 cccatggcgt tactattgat gatctaaaca caaaaatcag ccgagagcta tcgtcaaaat
     1201 acaggagaga gaagcaagtc atcagggcct tgcgcaacct ccgcgattac gggcaaaacc
     1261 gaccggtcgt tcgtgacatt gtcgaaaaca tatacggaaa ctcggtggat gagactacgc
     1321 gtacgttggc gctggagccg ttcatcgacg aagttcttcg aaaacttgct ggtgatggta
     1381 aagtgggttt ccaggttcgc gctggccaga aaaaatggtt tctaattggt aatgagcaat
     1441 caacggtggg tgggatgcgg caggtccagt accctgtcac acgcgccagc gcttaagcgg
     1501 atctcttatg tctttacgat ttatagtttt cattatcaag tatgcctata ttagtatata
     1561 gcatctttag atgacagtgt tcgaagtttc acgaataaaa gataatattc tactttttgc
     1621 tcccaccgcg tttgctagca cgagtgaaca ccatccctcg cctgtgagtt gtacccattc
     1681 ctctaaactg tagacatggt agcttcagca gtgttcgtta tgtacggcat cctccaacaa
     1741 acagtcggtt atagtttgtc ctgctcctct gaatcgtctc cctcgatatt tctcattttc
     1801 cttcgcatgc cagcattgaa atgatcgaag ttcaatgatg aaacggtaat tcttctgtca
     1861 tttactcatc tcatctcatc aagttatata attctatacg gatgtaattt ttcacttttc
     1921 gtcttgacgt ccaccctata atttcaatta ttgaaccctc acgatccagt tctccagtga
     1981 cacagccttt atctggtcaa acctttcttt ctaatcacct atgctgatgc ttaattaagg
     2041 gatttttgtc tccatcaacg gcatgcgccc aaaaatgacg ttttttttaa cccatagaca
     2101 cgaaactacc cattttccac cggcctgacc taccaccgga acaacggcca tctccaactt
     2161 gcaagttggg gaaattaaga gcatcgcagg tttaatggaa gaaaaaaaaa aggtacagca
     2221 cagcgcaaat ggagttagtt cccttatgtc acacactcac acacagtcgg tcagatcaag
     2281 catactgggt gcgtataaat agagtggcca ttgccaccct gtttatctca aaatctgtct
     2341 tgttagtggt cttctccctt tttcaggtta caattctctt gtttctactt agtatataag
     2401 tatatcaagc tatattaagc atactatcaa ctgtcaactc tatcctcaaa atacaataca
     2461 aaatggattc cgctgaacaa agagagtggt tctttcttga gaatttaggc ctacgagtca
     2521 ccctcatact tttatctgga atcaccgcct tgattgttga ttacagccgc atgctctatt
     2581 taagaaaaaa gatgccacct ggcccattac cctggccaat agccggaaac actctcagtc
     2641 tcccgaaaga gaggccttgg tacatggttg agcagctctc aaaagactac aagtccccag
     2701 taattacgtt ctggattgga cggttagtcc tcaatgagat ccttgtcaaa cgctccaaca
     2761 tctacaactc tagacgacac atgctcatgt ttggtgagct gatgaatgaa caagataacc
     2821 tcatacacaa atacacatac accaacgagc agcgggagag atttcgtgtc ctgcgcaaaa
     2881 tcacacatca tggtgttgga acacaacaag ttcaacaata ccgagaattt caagacgaca
     2941 agaacatggt gctggcatac gatatcctaa aaacacctga gctatttgct tcacgttttg
     3001 aacgctacgc ggcgggcgtt gtctctattg ttggattggg acgtcgtatt tccaacagtc
     3061 aagatacgtt gattacggaa gtcatcgctc agatgcaaca tgccgcacaa ctcgctgttg
     3121 tggcaaaaga ctggctacgg atcattgaga catttccttg gttggtaaag cttccccgta
     3181 gtatcgcacc ctggttacgc aatattaaac aatttagcaa aacaaagtac ggtcacgaaa
     3241 actacgcaaa attcctcttt cgcgaagccc cacaatacaa ccttagccgc ggcgaagtct
     3301 ccagtcttgt ctccaatctt tttggcgcag gcgcagatac atccagtagc actctcatca
     3361 cagctgtaat tgctatgcgc gccttccctg agacaatgaa acctgcctgg gacgaacttg
     3421 atcgcgtagc aggccactca agaagcccta cgcttgacga tgaattgcct tatgtccgcg
     3481 cctttgccaa agttttccgc tggcgcgcag tagccaacct cggaggaaaa actcatgcac
     3541 cgactcaaga cgactactgg aactgctact tcatacccaa gggaacttgg atacaaggga
     3601 acgtatgggc tattcatcac aacgagcgcg agtttccgga tcccgatcga tttaatccaa
     3661 gacggcgcct cgacacggaa gatctgaggc cgtttccggg agagaaggga tacatgacgt
     3721 ttggatgggg aggaggagtt gtgctggtca ggcgcttgct gagcagggca cgcatctcag
     3781 tgttgcgcgc ttgctttggg cctatcacgt tgagcctgct atcgacgaga aaacaggaag
     3841 tgagatacca gttgatatct tcagctatac taacggatca aatcgacgtc ctcagccttt
     3901 ctaggggagg aagcactgat gcgtctatca aaatacgaga ggccggcaaa gtatatgctt
     3961 gaacaaatcg ctcttaaata tatacctaaa gaacattaaa gctatattat aagcaaagat
     4021 acgtaaattt tgcttatatt attatacaca tatcatattt ctatattttt aagatttggt
     4081 tatataatgt acgtaatgca aaggaaataa attttataca ttattgaaca gcgtccaagt
     4141 aactacatta tgtgcactaa tagtttagcg tcgtgaagac tttattgtgt cgcgaaaagt
     4201 aaaaatttta aaaattagag caccttgaac ttgcgaaaaa ggttctcatc aactgtttaa
     4261 aaggaggata tcaggtccta tttctgacaa acaatataca aatttagttt caaagatgaa
     4321 tcagtgcgcg aaggacataa ctcattcaat tcaattcatc attttttttt tattcttttt
     4381 tttgatttcg gtttctttga aatttttttg attcggtaat ctccgaacag aaggaagaac
     4441 gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt gaagaaacat
     4501 gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg aaacgaagat
     4561 aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg
     4621 ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc
     4681 gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa
     4741 aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg
     4801 cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct gacattggta
     4861 atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta
     4921 cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcagaag
     4981 aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc
     5041 tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg
     5101 ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga
     5161 ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa cagtatagaa
     5221 ccgtggatga tgtggtctct acaggatctg acattattat tgttggaaga ggactatttg
     5281 caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat
     5341 atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat gcatgtatac
     5401 taaactcaca aattagagct tcaatttaat tatatcagtt attaccctat gcggtgtgaa
     5461 ataccgcaca gatgcgtaag gagaaaatac cgcatcagga taggaaaaaa ccgagcttcc
     5521 tttcatccgg cgcggctgtg ttctacatat cactgaagct ccgggtattt taagttatac
     5581 aagggaaaga tgccggctag actagcaagt tttaggctgc ttaacattat ggataggcgg
     5641 ataaagggcc caaacaggat tgtaaagctt agacgcttct ggttggacaa tggtacgttt
     5701 gtgtattaag taaggcttgg ctggggatag caacattggg cagagtatag aagaccacaa
     5761 aaaaaaggta tataagggca gagaagtctt tgtaatgtgt gtaacttctc ttccatgtgt
     5821 aatcagtatt tctacttact tcttaaatat acagaagtaa gacagataac caacagcctt
     5881 tcccagatat acatatatat ctttatttca gcttaaacaa taattatatt tgtttaactc
     5941 aaaaataaaa aaaaaaaacc aaactcacgc aactaattat tccataataa aataacaaca
     6001 tgagtgcctt ggagatatct agcgaagtat gtgacgttcc aaatggcacc catgggacga
     6061 agcgccgccg gagttcgact gtgctcaatc caaggagtta tgtcaaagag aagctttgga
     6121 cacaagctcc agtatttacg ggtagcacca actttgagcc acttgcagat gcgaagaaca
     6181 tcttgatcac tgggggcgcc ggcttcatag cctgctggtt cgtacgccat ctagtcctta
     6241 cgtaccccca ctataatgtg gtttccttgg acaaattgga ctactgcgca acactcaaca
     6301 acacgcgcat tctcgatagc cgaccgaact tcacattcga gcagggcgac atcacctcgc
     6361 ctgctactgt gaagcgtgtg ctccgtagac acaaaaccga caccattgtc cattttgcgg
     6421 cgcaatcgca tgttgacctc agctttggca actcgtacga gttcacgcat acaaatgttt
     6481 atggaacaca tgtcttgctc gagagagcga gggaacatgg tgtcaataaa ttcatacaca
     6541 tctcgacaga tgaggtgtat ggtgatgtgc ctgttggcgc agcagacctg ggcgaaacga
     6601 gcatactagc acccacaaat ccatactcgg ccagcaaagc agcggcggag atgatggtca
     6661 gcgcataccg gagcagcttc aagctacccc taattacggt ccgggcgaac aatgtatacg
     6721 ggccacatca atttcccgag aagattatac ccaaattcat catgctctta cagcgaaagc
     6781 gaaagctcct cctccacggc gacggctcac caacgcggcg ttacctctat gcaggagata
     6841 ttgtggatgc tctggacacc attttgcaca agggtgacat tggacaaatt tacaacattg
     6901 cctccaagga tgagatatcg aatacggaga tctgctaccg tctccttgat aatttcggca
     6961 tctcgcgaga tacgacgagc gaattaaaac aatggattca gtataccgag gatcgtccct
     7021 tcaacgacca gcggtacgca acggatggat cgaaacttgc tgcgctggga tggcagccca
     7081 agacaagctt cgaagaaggc cttcgcataa ctgtggattg gtatcgtaga tttggcgaag
     7141 tgtggtgggg cgatatcagc cgggttctga cgtcttttcc cgtggtaaca ggaaatgaaa
     7201 tcaggacgaa ggaggagcac gaagatttac catcgtccga cggtgagggg atacacgaag
     7261 aagatagtgc agtatggaca gtctagagtg cttttaacta agaattatta gtcttttctg
     7321 cttatttttt catcatagtt tagaacactt tatattaacg aatagtttat gaatctattt
     7381 aggtttaaaa attgatacag ttttataagt tactttttca aagactcgtg ctgtctattg
     7441 cataatgcac tggaagggga aaaaaaaggt gcacacgcgt ggctttttct tgaatttgca
     7501 gtttgaaaaa taactacatg gatgataaga aaacatggag tacagtcact ttgagaacct
     7561 tcaatcagct ggtaacgtct tcgttaattg gatactcaaa aaagatggat agcatgaatc
     7621 acaagatgga aggaaatgcg ggccacgacc acagtgatat gcatatggga gatggagatg
     7681 atacctccat tgggccgatg aagttagtcg acggatagaa gcggttgtcc cctttcccgg
     7741 cgagccggca gtcgggccga ggttcggata aattttgtat tgtgttttga ttctgtcatg
     7801 agtattactt atgttctctt taggtaaccc caggttaatc aatcacagtt tcataccggc
     7861 tagtattcaa attatgactt ttcttctgca gtgtcagcct tacgacgatt atctatgagc
     7921 tttgaatata gtttgccgtg attcgtatct ttaattggat aataaaatgc gaaggatcga
     7981 tgacccttat tattattttt ctacactggc taccgattta actcatcttc ttgaaagtat
     8041 ataagtaaca gtaaaatata ccgtacttct gctaatgtta tttgtccctt atttttcttt
     8101 tcttgtctta tgctatagta cctaagaata acgactattg ttttgaacta aacaaagtag
     8161 taaaagcaca taaaagaatt aagaaaatgc cacacgtaga aagcacaccc tccacctaca
     8221 tccccggccg cctcgacggc aaagtcgccc tcgtcactgg ctccggccgc ggcatcggcg
     8281 cagccgtagc cacgcatctg ggccgtctag gcgccaaagt ggtcgtcaac tacgccaact
     8341 cgaccaaaga tgctgaaaaa gtcgtctcag agatcaaagc gctgggcagc gacgccatcg
     8401 ccatcaaagc cgacatccgc caagtctcag acattgtccg cctcttcgac gaagccgtcg
     8461 cgcactttgg ccacctcgac atcgccgtca gcaactctgg cgtcgtaagc ttcggccact
     8521 tgaaagacgt gacagaagaa gaattcgacc gcgtcttcag cctcaacacg cgcggccagt
     8581 tcttcgtcgc ccgcgaggca taccgccatc tgaccgaggg cggccgcatc attctcactt
     8641 cctcaaacac ggcccgcgac ttcagcgtgc ccgagcactc gctctactcc gggtccaagg
     8701 gcgcaatcga ctcttttgta cgcatcttct caaaggactg tggcgataag aagattacgg
     8761 ttaatgcggt tgctcccggg ggcacggtga cggacatgtt tcatgaggtt tcgcatcatt
     8821 atattccgaa tggccttgag tatacggctg agcagcgtca gcagatggct gcgcatgcgt
     8881 cgccgttgca taggaatggg ttcccgcaag acgtggccaa tgtggtggga tttttggtta
     8941 gtaaggaggg tgagtgggtg aacggcaagg tccttacgct cgatggaggt gctgcataag
     9001 gagattgata agacttttct agttgcatat cttttatatt taaatcttat ctattagtta
     9061 attttttgta atttatcctt atatatagtc tggttattct aaaatatcat ttcagtatct
     9121 aaaaattccc ctcttttttc agttatatct taacaggcga cagtccaaat gttgatttat
     9181 cccagtccga ttcatcaggg ttgtgaagca ttttgtcaat ggtcgaaatc acatcagtaa
     9241 tagtgcctct tacttgcctc atagaatttc tttctcttaa cgtcaccgtt tggtctttta
     9301 tagtttcgaa atctatggtg ataccaaatg gtgttcccaa ttcatcgtta cgggcgtatt
     9361 ttttaccaat tgaagtattg gaatcgtcaa ttttaaagta tatctctctt ttacgtaaag
     9421 cctgcgagat cctcttaagt atagcgggga agccatcgtt attcgatatt gtcgtaacaa
     9481 atactttgat cggcgctatg cggccgccac cgcggtggag ctccagcttt tgttcccttt
     9541 agtgagggtt aattgcgcgc ttggcgagac tcaaacaaga tatattttgc ccgccttaca
     9601 gacgaaacta gttggaggtt atggagcata ctatcacgtg ggcggccact ggtgagttac
     9661 tacaccccag gggcaacgtt gatgctccta aaaaactctg gctggacgca agccgtaaca
     9721 cccgtgtcac ttcataatcg tttgcaattc agggcttgat ctacacatta ccctgttatc
     9781 cctattaatt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9841 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9901 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9961 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    10021 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    10081 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    10141 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    10201 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    10261 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    10321 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10381 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10441 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10501 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10561 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10621 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10681 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10741 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10801 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10861 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    10921 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10981 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    11041 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    11101 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    11161 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    11221 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    11281 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    11341 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    11401 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11461 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11521 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    11581 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    11641 ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt
    11701 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta
    11761 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc
    11821 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
    11881 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
    11941 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
    12001 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
    12061 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
    12121 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
    12181 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
    12241 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
    12301 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
    12361 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
    12421 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
    12481 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca
    12541 gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag
    12601 cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg
    12661 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt
    12721 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata
    12781 gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag
    12841 tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca
    12901 ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat
    12961 gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat gacattaacc
    13021 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
    13081 aacctcggcg cgccattacc ctgttatccc taatatacaa cgcaaagaag ctggaaaatt
    13141 ggcgagggaa tcctgtttct gtctatccaa gaatgggcat gaggtggcaa ccgtcgtgct
    13201 agcgtacagg gtgcactttg taaccatttg ggacaccgga cactcgctgt tttcgaaatt
    13261 accctttaag cgcgggtatt gaaccaggct tatgcccaag atcgtagcaa gcatttaaat
    13321 gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga
    13381 gttaattaa
//
