LOCUS       sequence-217954-        4360 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression of Omomer (tamoxifen inducible Omomyc) in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4360)
  TITLE     Nasi lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCS Omomyc-Mer
FEATURES             Location/Qualifiers
     source          1..4360
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      78..87
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1411..1545
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1435..1454)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1489..1508
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1658..1678)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1658..1676)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1697..1713)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1697..1713)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1710..1732)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1721..1737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1745..1751,1752..1769,1770..1775))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1790..1811
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1928..1945)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2099..2687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2179..2198)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2858..3649,3650..3718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3481..3500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3719..3823)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3849..4304)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3986..4007)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4198..4217
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agacttgttc
       61 tttttgcagg atctgccgcc accatgggga tcccatcgat gtcgaccgag gagaatgtca
      121 agaggcgaac acacaacgtc ttggagcgcc agaggaggaa cgagctaaaa cggagctttt
      181 ttgccctgcg tgaccagatc ccggagttgg aaaacaatga aaaggccccc aaggtagtta
      241 tccttaaaaa agccacagca tacatcctgt ccgtccaagc agagacgcaa aagctcattt
      301 ctgaaatcga cttgttgcgg aaacaaaacg aacagttgaa acacaaactt gaacagctac
      361 ggaactcttg tgcgtacagg gatccacgaa atgaaatggg tgcttcagga gacatgaggg
      421 ctgccaacct ttggccaagc cctcttgtga ttaagcacac taagaagaat agccctgcct
      481 tgtccttgac agctgaccag atggtcagtg ccttgttgga tgctgaaccg cccatgatct
      541 attctgaata tgatccttct agacccttca gtgaagcctc aatgatgggc ttattgacca
      601 acctagcaga tagggagctg gttcatatga tcaactgggc aaagagagtg ccaggctttg
      661 gggacttgaa tctccatgat caggtccacc ttctcgagtg tgcctggctg gagattctga
      721 tgattggtct cgtctggcgc tccatggaac acccggggaa gctcctgttt gctcctaact
      781 tgctcctgga caggaatcaa ggtaaatgtg tggaaggcat ggtggagatc tttgacatgt
      841 tgcttgctac gtcaagtcgg ttccgcatga tgaacctgca gggtgaagag tttgtgtgcc
      901 tcaaatccat cattttgctt aattccggag tgtacacgtt tctgtccagc accttgaagt
      961 ctctggaaga gaaggaccac atccaccgtg tcctggacaa gatcacagac actttgatcc
     1021 acctgatggc caaagctggc ctgactctgc agcagcagca tcgccgccta gctcagctcc
     1081 ttctcattct ttcccatatc cggcatatga gtaacaaacg catggagcat ctctacaaca
     1141 tgaaatgcaa gaacgtggta cccctctatg acctgctcct ggagatgttg gatgcccacc
     1201 gccttcatgc cccagccagt cgcatgggag tgcccccaga ggagcccagc cagacccagc
     1261 tggccaccac cagctccact tcagcacatt ccttacaaac ctactacata cccccggaag
     1321 cagagggctt ccccaacacg atctgagagc tcctactagt gaattcaagg cctctcgagc
     1381 ctctagaact atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag
     1441 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     1501 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     1561 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cgcggccgcg
     1621 gcgccaatgc attgggcccg gtacccagct tttgttccct ttagtgaggg ttaattgcgc
     1681 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     1741 cacacaacat acgagccggg agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1801 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1861 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1921 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1981 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2041 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2101 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2161 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2221 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2281 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2341 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2401 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2461 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2521 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2581 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2641 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2701 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2761 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2821 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2881 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2941 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3001 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3061 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3841 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     3901 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     3961 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     4021 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     4081 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     4141 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     4201 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     4261 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     4321 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
//
