LOCUS       sequence-218020-        9229 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Mammalian expression of hsOPA1 isoform 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Song Z, Chen H, Fiket M, Alexander C, Chan DC
  TITLE     OPA1 processing controls mitochondrial fusion and is regulated by 
            mRNA splicing, membrane potential, and Yme1L.
  JOURNAL   J Cell Biol. 2007 Aug 27;178(5):749-55. Epub 2007 Aug 20.
  PUBMED    17709429
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2007-08-27"; volume: "178(5)"; pages: "749-55. Epub 
            2007 Aug 20"
COMMENT     Sequence Label: OPA1-isoform2
FEATURES             Location/Qualifiers
     source          1..9229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     polyA_signal    4237..4358
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4248..4267)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4302..4321
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4372..4871
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4407..4430)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4804..4823
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4892..5491
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4892..4911)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5388..5408
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5591..6105
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6274..6290)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6274..6290)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6287..6309)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6298..6314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6322..6328,6329..6346,6347..6352))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6367..6388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6505..6522)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6676..7264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6756..6775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7435..8226,8227..8295))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8058..8077
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8296..8400)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8468..8486
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8524..8546)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8646..8665
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8859..8881
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9009..9028
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9228..9229,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga gaattcacgc gtggtaccat
     1441 gtggcgacta cgtcgggccg ctgtggcctg tgaggtctgc cagtctttag tgaaacacag
     1501 ctctggaata aaaggaagtt taccactaca aaaactacat ctggtttcac gaagcattta
     1561 tcattcacat catcctacct taaagcttca acgaccccaa ttaaggacat cctttcagca
     1621 gttctcttct ctgacaaacc ttcctttacg taaactgaaa ttctctccaa ttaaatatgg
     1681 ctaccagcct cgcaggaatt tttggccagc aagattagct acgagactct taaaacttcg
     1741 ctatctcata ctaggatcgg ctgttggggg tggctacaca gccaaaaaga cttttgatca
     1801 gtggaaagat atgataccgg accttagtga atataaatgg attgtgcctg acattgtgtg
     1861 ggaaattgat gagtatatcg attttggttc tccggaagaa acggcgttta gagcaacaga
     1921 tcgtggatct gaaagtgaca agcattttag aaaggtgtca gacaaagaga aaattgacca
     1981 acttcaggaa gaacttctgc acactcagtt gaagtatcag agaatcttgg aacgattaga
     2041 aaaggagaac aaagaattga gaaaattagt attgcagaaa gatgacaaag gcattcatca
     2101 tagaaagctt aagaaatctt tgattgacat gtattctgaa gttcttgatg ttctctctga
     2161 ttatgatgcc agttataata cgcaagatca tctgccacgg gttgttgtgg ttggagatca
     2221 gagtgctgga aagactagtg tgttggaaat gattgcccaa gctcgaatat tcccaagagg
     2281 atctggggag atgatgacac gttctccagt taaggtgact ctgagtgaag gtcctcacca
     2341 tgtggcccta tttaaagata gttctcggga gtttgatctt accaaagaag aagatcttgc
     2401 agcattaaga catgaaatag aacttcgaat gaggaaaaat gtgaaagaag gctgtaccgt
     2461 tagccctgag accatatcct taaatgtaaa aggccctgga ctacagagga tggtgcttgt
     2521 tgacttacca ggtgtgatta atactgtgac atcaggcatg gctcctgaca caaaggaaac
     2581 tattttcagt atcagcaaag cttacatgca gaatcctaat gccatcatac tgtgtattca
     2641 agatggatct gtggatgctg aacgcagtat tgttacagac ttggtcagtc aaatggaccc
     2701 tcatggaagg agaaccatat tcgttttgac caaagtagac ctggcagaga aaaatgtagc
     2761 cagtccaagc aggattcagc agataattga aggaaagctc ttcccaatga aagctttagg
     2821 ttattttgct gttgtaacag gaaaagggaa cagctctgaa agcattgaag ctataagaga
     2881 atatgaagaa gagttttttc agaattcaaa gctcctaaag acaagcatgc taaaggcaca
     2941 ccaagtgact acaagaaatt taagccttgc agtatcagac tgcttttgga aaatggtacg
     3001 agagtctgtt gaacaacagg ctgatagttt caaagcaaca cgttttaacc ttgaaactga
     3061 atggaagaat aactatcctc gcctgcggga acttgaccgg aatgaactat ttgaaaaagc
     3121 taaaaatgaa atccttgatg aagttatcag tctgagccag gttacaccaa aacattggga
     3181 ggaaatcctt caacaatctt tgtgggaaag agtatcaact catgtgattg aaaacatcta
     3241 ccttccagct gcgcagacca tgaattcagg aacttttaac accacagtgg atatcaagct
     3301 taaacagtgg actgataaac aacttcctaa taaagcagta gaggttgctt gggagaccct
     3361 acaagaagaa ttttcccgct ttatgacaga accgaaaggg aaagagcatg atgacatatt
     3421 tgataaactt aaagaggccg ttaaggaaga aagtattaaa cgacacaagt ggaatgactt
     3481 tgcggaggac agcttgaggg ttattcaaca caatgctttg gaagaccgat ccatatctga
     3541 taaacagcaa tgggatgcag ctatttattt tatggaagag gctctgcagg ctcgtctcaa
     3601 ggatactgaa aatgcaattg aaaacatggt gggtccagac tggaaaaaga ggtggttata
     3661 ctggaagaat cggacccaag aacagtgtgt tcacaatgaa accaagaatg aattggagaa
     3721 gatgttgaaa tgtaatgagg agcacccagc ttatcttgca agtgatgaaa taaccacagt
     3781 ccggaagaac cttgaatccc gaggagtaga agtagatcca agcttgatta aggatacttg
     3841 gcatcaagtt tatagaagac attttttaaa aacagctcta aaccattgta acctttgtcg
     3901 aagaggtttt tattactacc aaaggcattt tgtagattct gagttggaat gcaatgatgt
     3961 ggtcttgttt tggcgtatac agcgcatgct tgctatcacc gcaaatactt taaggcaaca
     4021 acttacaaat actgaagtta ggcgattaga gaaaaatgtt aaagaggtat tggaagattt
     4081 tgctgaagat ggtgagaaga agattaaatt gcttactggt aaacgcgttc aactggcgga
     4141 agacctcaag aaagttagag aaattcaaga aaaacttgat gctttcattg aagctcttca
     4201 tcaggagaaa taagcggccg cttcgagcag acatgataag atacattgat gagtttggac
     4261 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     4321 ctttatttgt aaccattata agctgcaata aacaagttaa cgaattctac cgggtagggg
     4381 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     4441 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     4501 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     4561 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     4621 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     4681 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     4741 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     4801 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     4861 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct
     4921 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     4981 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     5041 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     5101 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     5161 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     5221 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     5281 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     5341 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     5401 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     5461 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     5521 cgaaaggagc gcacgacccc atgcatcgat aaaataaaag attttattta gtctccagaa
     5581 aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt
     5641 ttgcaaggca tggaaaatac ataactgaga atagagaagt tcagatcaag gttaggaaca
     5701 gagagacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     5761 cagggccaag aacagatggt ccccagatgc ggtcccgccc tcagcagttt ctagagaacc
     5821 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
     5881 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     5941 gcccacaacc cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg
     6001 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     6061 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatgggta acagtttctt
     6121 gaagttggag aacaacattc tgagggtagg agtcgaatat taagtaatcc tgactcaatt
     6181 agccactgtt ttgaatccac atactccaat actcctgaaa tagttcatta tggacagcgc
     6241 agaagagctg gggagaatta attcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6301 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6361 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7081 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     7141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7321 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7381 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7441 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7501 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7561 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7621 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7681 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7741 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7801 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7861 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7921 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7981 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8041 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8101 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8161 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8221 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8281 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8341 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8401 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     8461 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     8521 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     8581 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     8641 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     8701 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     8761 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     8821 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     8881 gacggcgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
     8941 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     9001 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     9061 ccggccacga tgcgtccggc gtagaggcga ttagtccaat ttgttaaaga caggatatca
     9121 gtggtccagg ctctagtttt gactcaacaa tatcaccagc tgaagcctat agagtacgag
     9181 ccatagataa aataaaagat tttatttagt ctccagaaaa aggggggaa
//
