LOCUS       sequence-218614-       10540 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Express inducible BCCP-PupE in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Liu Q, Zheng J, Sun W, Huo Y, Zhang L, Hao P, Wang H, Zhuang M
  TITLE     A proximity-tagging system to identify membrane protein-protein 
            interactions.
  JOURNAL   Nat Methods. 2018 Aug 13. pii: 10.1038/s41592-018-0100-5. doi: 
            10.1038/s41592-018-0100-5.
  PUBMED    30104635
REFERENCE   2  (bases 1 to 10540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Aug 13. pii: 10.1038/s41592-018-0100-5. doi: 
            10.1038/s41592-018-0100-5."
COMMENT     Sequence Label: pLVX-TRE3G-BCCP-PupE-IRES-BFP
FEATURES             Location/Qualifiers
     source          1..10540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     40..64
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    614..1163
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(758..775)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     985..1004
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1166..1867
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    1927..2515
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1980..2000)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2398..2409)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2518..2534)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2519..2535)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2625..2858
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2883..2901)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2884..2901)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3191..3210)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3424..3443)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3543..3565
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3603..3621)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3689..3793
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3794..3862,3863..4654)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4012..4031)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4825..5413
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5314..5333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5567..5584
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5654..5674)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5659..5988
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5839..5974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7122..7187
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7317..7337
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7762..7896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7799..7818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7853..7872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8064..8697
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8744..8869
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9366..9599
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9784..9828
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10113..10230
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(10292..10540,1..101)
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    10300..10318
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10336..10354
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10372..10390
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10408..10426
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10444..10462
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10480..10498
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10516..10534
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gtcgaggtag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag tgaaccgtca
       61 gatcgcctgg agcaattcca caacactttt gtcttatact tacgcgtctg caggatatca
      121 agcttgcggt accgcgggcc cggggccacc atggccggga aggcaggcga gggagagatc
      181 cccgcaccct tggccggcac ggtcagcaaa atcctggtca aggaaggcga caccgtgaag
      241 gctggacaga cggtgttggt actggaggcg atgaagatgg agacagagat caatgccccg
      301 accgatggga aggtggagaa ggtgttggtt aaggaaaggg acgccgtgca gggcggtcag
      361 ggactgatca agatcggcga ctacgacatc ccgacaaccg ccagcatgaa cgcgaaacag
      421 acccagatca tgggtggcgg tggtcgtgac gaagacaatg cggaagactc tgctcaggcg
      481 tctggtcagg ttcagatcaa taccgaaggt gttgactctc tgctggacga aatcgacggc
      541 ctgctcgaaa acaacgcgga ggaattcgtt cgttcttacg ttcagaaagg tggtgaataa
      601 gccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
      661 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
      721 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
      781 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
      841 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
      901 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
      961 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     1021 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     1081 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     1141 ttcctttgaa aaacacgata ataccatgag cgagctgatt aaggagaaca tgcacatgaa
     1201 gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg
     1261 caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc
     1321 cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca
     1381 cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca catgggagag
     1441 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga
     1501 cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt
     1561 gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc ccgctgacgg
     1621 cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc
     1681 aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt
     1741 ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga cctacgtcga
     1801 gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct
     1861 taattaggcg gccgcgactc tagagtcgac ctgcaggcat gcaagcttga tatcaagctt
     1921 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     1981 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2041 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2101 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2161 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2221 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2281 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     2341 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2401 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     2461 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     2521 taccgtcgac ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa
     2581 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     2641 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     2701 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     2761 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     2821 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     2881 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     2941 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     3001 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     3061 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     3121 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     3181 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     3241 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     3301 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     3361 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     3421 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     3481 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     3541 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     3601 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     3661 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3721 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3781 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3841 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3901 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3961 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4021 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4081 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4141 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4201 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4261 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4321 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4381 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4441 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4501 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4561 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4621 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4681 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4741 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4801 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4861 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4921 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4981 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5041 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5101 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5161 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5221 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5281 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5341 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5401 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5461 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5521 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     5581 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     5641 ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5701 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5761 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5821 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5881 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5941 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac
     6001 aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag
     6061 tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa
     6121 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
     6181 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
     6241 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
     6301 cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
     6361 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
     6421 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
     6481 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
     6541 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
     6601 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
     6661 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
     6721 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
     6781 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
     6841 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
     6901 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
     6961 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
     7021 caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga
     7081 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     7141 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     7201 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     7261 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     7321 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     7381 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     7441 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     7501 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     7561 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     7621 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     7681 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     7741 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     7801 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7861 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     7921 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     7981 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     8041 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     8101 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     8161 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     8221 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     8281 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     8341 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     8401 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     8461 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     8521 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8581 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8641 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     8701 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     8761 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     8821 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     8881 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     8941 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     9001 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     9061 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     9121 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     9181 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     9241 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     9301 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     9361 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     9421 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     9481 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     9541 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     9601 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     9661 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     9721 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     9781 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     9841 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     9901 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     9961 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    10021 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
    10081 cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
    10141 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
    10201 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
    10261 gtttatcgat gaggcccttt cgtcttcact cgagtttact ccctatcagt gatagagaac
    10321 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca
    10381 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt
    10441 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac
    10501 gtatatccag tttactccct atcagtgata gagaacgtat
//
