LOCUS       sequence-218625-        6240 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 400 / 490, Emission: 
            468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6240)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-ALK1-N-13
FEATURES             Location/Qualifiers
     source          1..6240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2247..2963
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    3086..3207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3123..3142)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3177..3196
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3214..3669)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3351..3372)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3563..3582
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3696..3800
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3802..4159
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3802..3993
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3825..3845)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4010..4145
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4194..4988
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4194..4988
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4248..4267)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4858..4877
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5176..5195)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5220..5267
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5596..6184
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6085..6104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggactcagat ctcgagctca agcttcgaat tcaccgccat gaccttgggc tcccccagga
      721 aaggccttct gatgctgctg atggccttgg tgacccaggg agaccctgtg aagccgtctc
      781 ggggcccgct ggtgacctgc acgtgtgaga gcccacattg caaggggcct acctgccggg
      841 gggcctggtg cacagtagtg ctggtgcggg aggaggggag gcacccccag gaacatcggg
      901 gctgcgggaa cttgcacagg gagctctgca gggggcgccc caccgagttc gtcaaccact
      961 actgctgcga cagccacctc tgcaaccaca acgtgtccct ggtgctggag gccacccaac
     1021 ctccttcgga gcagccggga acagatggcc agctggccct gatcctgggc cccgtgctgg
     1081 ccttgctggc cctggtggcc ctgggtgtcc tgggcctgtg gcatgtccga cggaggcagg
     1141 agaagcagcg tggcctgcac agcgagctgg gagagtccag tctcatcctg aaagcatctg
     1201 agcagggcga cagcatgttg ggggacctcc tggacagtga ctgcaccaca gggagtggct
     1261 cagggctccc cttcctggtg cagaggacag tggcacggca ggttgccttg gtggagtgtg
     1321 tgggaaaagg ccgctatggc gaagtgtggc ggggcttgtg gcacggtgag agtgtggccg
     1381 tcaagatctt ctcctcgagg gatgaacagt cctggttccg ggagactgag atctataaca
     1441 cagtgttgct cagacacgac aacatcctag gcttcatcgc ctcagacatg acctcccgca
     1501 actcgagcac gcagctgtgg ctcatcacgc actaccacga gcacggctcc ctctacgact
     1561 ttctgcagag acagacgctg gagccccatc tggctctgag gctagctgtg tccgcggcat
     1621 gcggcctggc gcacctgcac gtggagatct tcggtacaca gggcaaacca gccattgccc
     1681 accgcgactt caagagccgc aatgtgctgg tcaagagcaa cctgcagtgt tgcatcgccg
     1741 acctgggcct ggctgtgatg cactcacagg gcagcgatta cctggacatc ggcaacaacc
     1801 cgagagtggg caccaagcgg tacatggcac ccgaggtgct ggacgagcag atccgcacgg
     1861 actgctttga gtcctacaag tggactgaca tctgggcctt tggcctggtg ctgtgggaga
     1921 ttgcccgccg gaccatcgtg aatggcatcg tggaggacta tagaccaccc ttctatgatg
     1981 tggtgcccaa tgaccccagc tttgaggaca tgaagaaggt ggtgtgtgtg gatcagcaga
     2041 cccccaccat ccctaaccgg ctggctgcag acccggtcct ctcaggccta gctcagatga
     2101 tgcgggagtg ctggtaccca aacccctctg cccgactcac cgcgctgcgg atcaagaaga
     2161 cactacaaaa aattagcaac agtccagaga agcctaaagg gattcaaggc agcgcaggcg
     2221 gaagcgggga tccaccggtc gccaccatga gcaagggcgc cgagctgttc accggcatcg
     2281 tgcccatcct gatcgagctg aatggcgatg tgaatggcca caagttcagc gtgagcggcg
     2341 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2401 agctgcctgt gccctggccc accctggtgg ccaccctgag ctacggcgtg cagtgcttct
     2461 cacgctaccc cgatcacatg aagcagcacg acttcttcaa gagcgccatg cctgagggct
     2521 acatccagga gcgcaccatc ttcttcgagg atgacggcaa ctacaagacg cgcgccgagg
     2581 tgaagttcga gggcgatacc ctggtgagtc gcatcgagct gaccggcact gatttcaagg
     2641 aggatggcaa catcctgggc aataagatgg agtacaacta caacgccacc aatgtgtaca
     2701 tcgtggccga caaggccagg aatggcatca aggtgaactt caaggtccgc cacaacatca
     2761 aggatggcag cgtgcagctg gccgaccact accagcagaa tacccccatc ggcgatggcc
     2821 ctgtgctgct gcccgataac cactacctgt ccacccagag cgccctgtcc aaggacccca
     2881 acgagaagcg cgatcacatg atctacctcg agttcgtgac cgccgccgcc atcacccacg
     2941 gcatggatga gctgtacaag taaagcggcc gcgactctag atcataatca gccataccac
     3001 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     3061 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     3121 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3181 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
     3241 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     3301 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     3361 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     3421 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     3481 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     3541 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     3601 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     3661 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3721 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3781 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     3841 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3901 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3961 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4021 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4081 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4141 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     4201 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     4261 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     4321 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     4381 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     4441 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     4501 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     4561 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     4621 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     4681 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     4741 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     4801 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     4861 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     4921 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     4981 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     5041 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     5101 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     5161 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     5221 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     5281 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     5341 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     5401 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
     5461 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5521 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5581 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5641 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5701 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5761 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5821 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5881 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5941 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6001 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6061 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6121 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6181 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
//
