LOCUS       sequence-218630-        6231 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 400 / 490, Emission: 
            468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6231)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-ALK1-C-18
FEATURES             Location/Qualifiers
     source          1..6231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             667..1380
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    3073..3194
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3110..3129)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3164..3183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3201..3656)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3338..3359)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3550..3569
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3683..3787
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3789..4146
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3789..3980
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3812..3832)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3997..4132
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4181..4975
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4181..4975
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4235..4254)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4845..4864
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5163..5182)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5207..5254
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5583..6171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6072..6091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggtc
      661 gccaccatga gcaagggcgc cgagctgttc accggcatcg tgcccatcct gatcgagctg
      721 aatggcgatg tgaatggcca caagttcagc gtgagcggcg agggcgaggg cgatgccacc
      781 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcctgt gccctggccc
      841 accctggtgg ccaccctgag ctacggcgtg cagtgcttct cacgctaccc cgatcacatg
      901 aagcagcacg acttcttcaa gagcgccatg cctgagggct acatccagga gcgcaccatc
      961 ttcttcgagg atgacggcaa ctacaagacg cgcgccgagg tgaagttcga gggcgatacc
     1021 ctggtgagtc gcatcgagct gaccggcact gatttcaagg aggatggcaa catcctgggc
     1081 aataagatgg agtacaacta caacgccacc aatgtgtaca tcgtggccga caaggccagg
     1141 aatggcatca aggtgaactt caaggtccgc cacaacatca aggatggcag cgtgcagctg
     1201 gccgaccact accagcagaa tacccccatc ggcgatggcc ctgtgctgct gcccgataac
     1261 cactacctgt ccacccagag cgccctgtcc aaggacccca acgagaagcg cgatcacatg
     1321 atctacctcg agttcgtgac cgccgccgcc atcacccacg gcatggatga gctgtacaag
     1381 tccggactca gatctcgagc tcaagcttcg aattcagggt ctggaagtgg tagcaccttg
     1441 ggctccccca ggaaaggcct tctgatgctg ctgatggcct tggtgaccca gggagaccct
     1501 gtgaagccgt ctcggggccc gctggtgacc tgcacgtgtg agagcccaca ttgcaagggg
     1561 cctacctgcc ggggggcctg gtgcacagta gtgctggtgc gggaggaggg gaggcacccc
     1621 caggaacatc ggggctgcgg gaacttgcac agggagctct gcagggggcg ccccaccgag
     1681 ttcgtcaacc actactgctg cgacagccac ctctgcaacc acaacgtgtc cctggtgctg
     1741 gaggccaccc aacctccttc ggagcagccg ggaacagatg gccagctggc cctgatcctg
     1801 ggccccgtgc tggccttgct ggccctggtg gccctgggtg tcctgggcct gtggcatgtc
     1861 cgacggaggc aggagaagca gcgtggcctg cacagcgagc tgggagagtc cagtctcatc
     1921 ctgaaagcat ctgagcaggg cgacagcatg ttgggggacc tcctggacag tgactgcacc
     1981 acagggagtg gctcagggct ccccttcctg gtgcagagga cagtggcacg gcaggttgcc
     2041 ttggtggagt gtgtgggaaa aggccgctat ggcgaagtgt ggcggggctt gtggcacggt
     2101 gagagtgtgg ccgtcaagat cttctcctcg agggatgaac agtcctggtt ccgggagact
     2161 gagatctata acacagtgtt gctcagacac gacaacatcc taggcttcat cgcctcagac
     2221 atgacctccc gcaactcgag cacgcagctg tggctcatca cgcactacca cgagcacggc
     2281 tccctctacg actttctgca gagacagacg ctggagcccc atctggctct gaggctagct
     2341 gtgtccgcgg catgcggcct ggcgcacctg cacgtggaga tcttcggtac acagggcaaa
     2401 ccagccattg cccaccgcga cttcaagagc cgcaatgtgc tggtcaagag caacctgcag
     2461 tgttgcatcg ccgacctggg cctggctgtg atgcactcac agggcagcga ttacctggac
     2521 atcggcaaca acccgagagt gggcaccaag cggtacatgg cacccgaggt gctggacgag
     2581 cagatccgca cggactgctt tgagtcctac aagtggactg acatctgggc ctttggcctg
     2641 gtgctgtggg agattgcccg ccggaccatc gtgaatggca tcgtggagga ctatagacca
     2701 cccttctatg atgtggtgcc caatgacccc agctttgagg acatgaagaa ggtggtgtgt
     2761 gtggatcagc agacccccac catccctaac cggctggctg cagacccggt cctctcaggc
     2821 ctagctcaga tgatgcggga gtgctggtac ccaaacccct ctgcccgact caccgcgctg
     2881 cggatcaaga agacactaca aaaaattagc aacagtccag agaagcctaa agtgattcaa
     2941 tagggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
     3001 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     3061 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3121 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3181 atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     3241 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     3301 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     3361 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     3421 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     3481 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     3541 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     3601 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     3661 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3721 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3781 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     3841 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3901 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3961 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4021 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4081 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4141 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     4201 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     4261 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     4321 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     4381 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     4441 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     4501 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     4561 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     4621 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     4681 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     4741 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     4801 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     4861 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     4921 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     4981 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     5041 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     5101 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
     5161 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     5221 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     5281 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     5341 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     5401 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
     5461 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5521 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5581 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5641 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5701 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5761 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5821 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5881 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5941 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6001 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6061 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6121 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6181 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat g
//
