LOCUS       sequence-218651-       12032 bp    DNA     circular SYN 14-APR-2025
DEFINITION  galactose-inducible Ty3-his3AI; additional PPT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12032)
  TITLE     Galactose-inducible Ty3-his3AI
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPS3858
FEATURES             Location/Qualifiers
     source          1..12032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    190..307
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     promoter        complement(6208..6394)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     misc_feature    7499..8661
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      7824..8661
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     CDS             complement(8996..9799)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9800..10015)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(10040..10058)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10126..10230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10231..10299,10300..11091)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10449..10468)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11262..11850
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11751..11770
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12004..12021
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gttaacccgc ggatcaaaaa tcatcgcttc gctgattaat taccccagaa ataaggctaa
       61 aaaactaatc gcattatcat cctatggttg ttaatttgat tcgttcattt gaaggtttgt
      121 ggggccaggt tactgccaat ttttcctctt cataaccata aaagctagta ttgtagaatc
      181 tttattgttc ggagcagtgc ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga
      241 agacgaggac gcacggagga gagtctttct tcggagggct gtcacccgct cggcggcttc
      301 taatccgtac ttcaatatag caatgagcag ttaagcgtat tactgaaagt tccaaagaga
      361 aggttttttt aggctaatcg acctcgactc tagaggatcc ccctgaacta cccaaagtat
      421 aaatgcctga acaattagtt tagatccgag attccgcgct tccaccactt agtatgattc
      481 atattttata taatatataa gataagtaac attccgtgaa ttaatctgat aaactgtttt
      541 gacaactggt tacttcccta agactgttta tattaggatt gtcaagacac tccggtatta
      601 ctcgagcccg taatacaaca cctggtagcg ttaaaggtta ctaattgttc aaacgaacca
      661 tcgaaaagcc gaacctagct acaccacacc ccagtatgag ctttatggat caaatcccag
      721 gaggaggaaa ttatccaaaa ctcccagtag aatgccttcc taacttcccg atccaaccat
      781 ctttgacctt cagaggtaga aatgactcgc ataaactgaa aaactttatc tccgaaataa
      841 tgttaaacat gtctatgata tcttggccga atgatgccag tcgtattgtg tactgcagaa
      901 gacatttatt aaaccccgct gctcagtggg ctaatgactt tgtacaagaa caaggtatac
      961 ttgaaataac attcgacaca ttcatacaag gattatatca gcatttctat aagccaccag
     1021 atatcaataa aatctttaat gcaatcacgc aactttccga agctaaactt ggtattgagc
     1081 gtctcaacca acgattcaga aagatttggg acagaatgcc accagacttc atgaccgaaa
     1141 aagctgccat aatgacatat actaggctat tgacaaagga aacctataat attgtcagaa
     1201 tgcacaaacc agagacatta aaagacgcca tggaagaggc ttaccagaca actgcactaa
     1261 ctgaaagatt cttcccagga ttcgaacttg atgctgatgg agacactatc atcggtgcca
     1321 caacccactt acaagaagaa tacgactctg actatgattc agaagataat ctgacccaga
     1381 atggatacgt ccataccgta aggacaagaa gatcttacaa taaaccaatg tcaaatcatc
     1441 gaaacaggag aaataacaac ccatctagag aagaatgtat aaaaaatcgg ctatgcttct
     1501 attgtaagaa agagggacat cgcctgaacg aatgtagagc acgtaaggcg agttctaacc
     1561 gatcttgaac tcgaatcaaa agaccaacaa actcctttta tcaaaacctt accaattgta
     1621 cactatatcg ccatccccga gatggacaat accgccgaaa aaaccataaa aatacaaaac
     1681 acgaaagtaa aaaccctgtt tgacagtgga tcacccacgt catttatccg aagagatatt
     1741 gtagaacttc tcaaatacga aatctacgag acccctccac tccgttttag aggattcgta
     1801 gccaccaaat ccgccgttac atccgaagca gtcaccattg acctcaaaat caatgacctg
     1861 catataactt tagccgcgta catactggat aacatggact accaattgtt aattggaaat
     1921 ccaatcttac gccgctaccc gaaaatcctg cacacagtac tgaataccag agagagcccc
     1981 gactccttaa agcccaagac ttatcgctcc gaaaccgtta ataacgttag aacctactcc
     2041 gctggtaatc gtggtaaccc cagaaacata aaactgtctt ttgcccccac cattctcgaa
     2101 gcaactgacc cgaaatccgc tggtaatcgt ggtgactcca gaaccaaaac cctgtctctt
     2161 gcaaccacta ctcctgcagc aattgacccg cttacgaccc ttgataaccc aggtagtact
     2221 caaagtacat ttgcgcaatt cccgatacct gaagaagcga gcatcctaga agaggatgga
     2281 aaatactcca acgttgtctc aaccattcag agtgtagaac ctaatgctac tgatcacagc
     2341 aataaggaca ccttttgcac tttgccagtt tggttacaac agaagtatag agagatcata
     2401 cgtaatgatc tcccaccaag acctgccgac attaataaca tccccgtaaa acatgatatt
     2461 gaaattaaac ctggcgcaag actacctcga ctacagccat accatgttac agaaaagaac
     2521 gaacaagaaa tcaacaaaat agttcaaaaa ctgctcgata acaagttcat tgttccctca
     2581 aagtcgcctt gcagctcccc tgtagtcctc gtcccgaaga aagacggtac cttccgactc
     2641 tgcgtcgatt accgcaccct gaacaaagct accatctccg acccattccc attacccaga
     2701 atcgacaacc tattgagccg tattggaaat gcccagatat ttaccacgct agatttgcat
     2761 agtggttacc accagatccc gatggaaccc aaagaccgct acaaaaccgc ctttgtcaca
     2821 ccatccggta agtatgaata taccgtcatg ccatttggct tagtcaatgc acctagtaca
     2881 ttcgcaagat acatggctga tacatttaga gacctgagat tcgtcaatgt ttaccttgat
     2941 gatatattaa tattctccga atctccagaa gaacattgga aacatttaga cacggtacta
     3001 gaaagattaa agaacgagaa cctcattgtt aagaagaaaa aatgtaaatt tgcatctgaa
     3061 gaaactgagt ttttaggcta tagtattgga atccagaaaa tagctccact acagcacaaa
     3121 tgtgcagcaa tccgagactt tccgacgcct aaaacagtaa aacaagcaca gagattttta
     3181 ggaatgatta attactacag acgattcatt ccaaattgct ccaagattgc acagccaatc
     3241 caactgttta tttgtgacaa aagtcaatgg acagaaaaac aagacaaggc aattgataaa
     3301 ctaaaagacg ccttgtgtaa ctcccccgtc ctagtaccat tcaacaacaa agcaaactac
     3361 cgacttacaa cagacgcctc aaaagacggc attggtgctg ttctagaaga agtcgacaac
     3421 aagaacaaac ttgttggtgt cgtcggttac ttctctaaat ccttagagag tgcccagaaa
     3481 aactatcctg ctggcgaatt agaactactt ggaattatca aagcactcca ccacttccga
     3541 tatatgcttc acggaaagca tttcacgtta agaacagacc acattagttt gttatcatta
     3601 caaaacaaga acgaacccgc acgacgcgtg caacgctggt tagatgacct agccacatat
     3661 gacttcacct tagaatacct agctggaccc aagaacgttg tcgcagatgc catatcccgt
     3721 gccgtatata ctataacccc cgaaacatcc cgacctatcg acacagaaag ctggaaatct
     3781 tactacaaat cagacccatt atgtagtgct gtcttaattc atatgaaaga attgacacaa
     3841 cacaacgtca cacctgaaga tatgtcagcc ttccgtagtt accagaagaa actcgaacta
     3901 tcagagacct tccgaaagaa ttattcccta gaagacgaaa tgatctatta ccaagaccga
     3961 ctagtagtac caataaaaca acagaacgca gttatgagac tatatcatga ccatacctta
     4021 tttggaggac attttggtgt aacagtgacc cttgcgaaaa tcagcccaat ttactattgg
     4081 ccaaaattac aacattcgat catacaatac atcaggacct gcgtacaatg tcaactaata
     4141 aaatcacacc gaccacgctt acatggacta ttacaaccac tccctatagc agaaggaaga
     4201 tggcttgata tatcaatgga ttttgtgaca ggattacccc cgacatcaaa taacttgaat
     4261 atgatcctcg tcgtagttga tcgtttttcg aaacgcgctc acttcatagc tacaaggaaa
     4321 accttagacg caacacaact aatagatcta ctctttcgat acattttttc atatcatggt
     4381 tttcccagga caataaccag tgatagagat gtccgtatga ccgccgacaa atatcaagaa
     4441 ctcacgaaaa gactaggaat aaaatcgaca atgtcttccg cgaaccaccc ccaaacagat
     4501 ggacaatccg aacgaacgat acagacatta aacaggttac taagagccta tgcttcaacc
     4561 aatattcaga attggcatgt atatttacca caaatcgaat ttgtttacaa ttctacacct
     4621 actagaacac ttggaaaatc accatttgaa attgatttag gatatttacc gaatacccct
     4681 gctattaagt cagatgacga agtcaacgca agaagtttta ctgccgtaga acttgccaaa
     4741 cacctcaaag cccttaccat ccaaacgaag gaacagctag aacacgctca aatcgaaatg
     4801 gaaactaata acaatcaaag acgtaaaccc ttattgttaa acataggaga tcacgtatta
     4861 gtgcatagag atgcatactt caagaaaggt gcttatatga aagtacaaca aatatacgtc
     4921 ggaccatttc gagttgtcaa gaaaataaac gataacgcct acgaactaga tttaaactct
     4981 cacaagaaaa agcacagagt tattaatgta caattcctga aaaagtttgt ataccgtcca
     5041 gacgcgtacc caaagaataa accaatcagc tccactgaaa gaattaagag agcacacgaa
     5101 gttactgcac tcataggaat agatactaca cacaaaactt acttatgtca catgcaagat
     5161 gtagacccaa cactttcagt agaatactca gaagctgaat tttgccaaat tcccgaaaga
     5221 acacgaagat caatattagc caactttaga caactctacg aaacacaaga caaccctgag
     5281 agagaggaag atgttgtatc tcaaaatgag atatgtcagt atgacaatac gtcaccctaa
     5341 taagagctcg gttcaagggt gagagtatgt gtcgaatcat cgcctagacc gaaatgcctt
     5401 aaggggaagc ttgggctgca gctttaaata atcggtgtca ctacataaga acacctttgg
     5461 tggagggaac atcgttggta ccattgggcg aggtggcttc tcttatggca accgcaagag
     5521 ccttgaacgc actctcacta cggtgatgat cattcttgcc tcgcagacaa tcaacgtgga
     5581 gggtaattct gctagcctct gcaaagcttt caagaaaatg cgggatcatc tcgcaagaga
     5641 gatctcctac tttctccctt tgcaaaccaa gttcgacaac tgcgtacggc ctgttcgaaa
     5701 gatctaccac cgctctggaa agtgcctcat ccaaaggcgc aaatcctgat ccaaaccttt
     5761 ttactccacg cacggcccct agggcctctt taaaagcttg accgagagca atcccgcagt
     5821 cttcagtggt gtgatggtcg tctatgtgta agtcaccaat gcactcaacg attagcgacc
     5881 agccggaatg cttgggtatg ttaatatgga ctaaaggagg cttttctgca ggtcgactct
     5941 agaggatccc cgggtaccga gctcgaattt ttactaacaa atggtattat ttataacagc
     6001 cagagcatgt atcatatggt ccagaaaccc tatacctgtg tggacgttaa tcacttgcga
     6061 ttgtgtggcc tgttctgcta ctgcttctgc ctctttttct gggaagatcg agtgctctat
     6121 cgctagggga ccacccttta aagagatcgc aatctgaatc ttggtttcat ttgtaatacg
     6181 ctttactagg gctttctgct ctgtcatctt tgccttcgtt tatcttgcct gctcattttt
     6241 tagtatattc ttcgaagaaa tcacattact ttatataatg tataattcat tatgtgataa
     6301 tgccaatcgc taagaaaaaa aaagagtcat ccgctaggtg gaaaaaaaaa atgaaaatca
     6361 ttaccgaggc ataaaaaaat atagagtgta ctagaggctc caagagtgta ctagaggagg
     6421 ccaagagtac ctgcaggtcc tagtcgcacg cttgtagtgg atcgtctgca taatatcgtg
     6481 tgacccgggt gagagagagg aagatgttgt atctcaaaat gagatatgtc agtatgacaa
     6541 tacgtcaccc tgaacgttca taaaacacat atgaaacaac cttataacaa aacgaacaac
     6601 atgagacaaa acccgacctt ccctagctga actacccaaa gtataaatgc ctgaacaatt
     6661 agtttagatc cgagattccg cgcttccacc acttagtatg attcatattt tatataatat
     6721 ataagataag taacattccg tgaattaatc tgataaactg ttttgacaac tggttacttc
     6781 cctaagactg tttatattag gattgtcaag acactccggt attactcgag cccgtaatac
     6841 aacaggcggc cgcgaattcc tttctgcatg ccacgttcgc ggcgcctgat gcggtatttt
     6901 ctccttacgc atctgtgcgg tatttcacac cgcataatcg ctgggccatt ctcatgaaga
     6961 atatcttgaa tttattgtca tattactagt tggtgtggaa gtcctaatat cggtgatcaa
     7021 tatagtggtt gacatgctgg ctagtcaaca ttgagccttt tgatcatgca aatatattac
     7081 ggtattttac aatcaaatat caaacttaac tattgacttt ataacttatt taggtggtaa
     7141 cattcttata aaaaagaaaa aaattactgc aaaacagtac tagcttttaa cttgtatcct
     7201 aggttatcta tgctgtctca ccatagagaa tattacctat ttcagaatgt atgtccatga
     7261 ttcgccgggt aaatacatat aatacacaaa tctggcttaa taaagtctat aatatatctc
     7321 ataaagaagt gctaaattgg ctagtgctat atatttttaa gaaaatttct tttgactaag
     7381 tccatatcga ctttgtaaaa gttcacatta gcatacatat attacacgag ccagaaatag
     7441 taacttttgc ctaaatcaca aattgcaaaa tttaattgct tgcaaaaggt cacatgctta
     7501 taatcaactt ttttaaaaat ttaaaatact tttttatttt ttatttttaa acataaatga
     7561 aataatttat ttattgttta tgattaccga aacataaaac ctgctcaaga aaaagaaact
     7621 gttttgtcct tggaaaaaaa gcactaccta ggagcggcca aaatgccgag gctttcatag
     7681 cttaaactct ttacagaaaa taggcattat agatcagttc gagttttctt attcttcctt
     7741 ccggttttat cgtcacagtt ttacagtaaa taagtatcac ctcttagagt tcgatgataa
     7801 gctgtcaaac atgagaatta attccacatg ttaaaatagt gaaggagcat gttcggcaca
     7861 cagtggaccg aacgtggggt aagtgcacta gggtccggtt aaacggatct cgcattgatg
     7921 aggcaacgct aattatcaac atatagattg ttatctatct gcatgaacac gaaatcttta
     7981 cttgacgact tgaggctgat ggtgtttatg caaagaaacc actgtgttta atatgtgtca
     8041 ctgtttgata ttactgtcag cgtagaagat aatagtaaaa gcggttaata agtgtatttg
     8101 agataagtgt gataaagttt ttacagcgaa aagacgataa atacaagaaa atgattacga
     8161 ggatacggag agaggtatgt acatgtgtat ttatatacta agctgccggc ggttgtttgc
     8221 aagaccgaga aaaggctagc aagaatcggg tcattgtagc gtatgcgcct gtgaacattc
     8281 tcttcaacaa gtttgattcc attgcggtga aatggtaaaa gtcaaccccc tgcgatgtat
     8341 attttcctgt acaatcaatc aaaaagccaa atgatttagc attatcttta catcttgtta
     8401 ttttacagat tttatgttta gatcttttat gcttgctttt caaaaggctt gcaggcaagt
     8461 gcacaaacaa tacttaaata aatactactc agtaataacc tatttcttag catttttgac
     8521 gaaatttgct attttgttag agtcttttac accatttgtc tccacacctc cgcttacatc
     8581 aacaccaata acgccattta atctaagcgc atcaccaaca ttttctggcg tcagtccacc
     8641 agctaacata aaatgtaagc tctcggggct ctcttgcctt ccaacccagt cagaaatcga
     8701 gttccaatcc aaaagttcac ctgtcccacc tgcttctgaa tcaaacaagg gaataaacga
     8761 atgaggtttc tgtgaagctg cactgagtag tatgttgcag tcttttggaa atacgagtct
     8821 tttaataact ggcaaaccga ggaactcttg gtattcttgc cacgactcat ctccatgcag
     8881 ttggacgatc gatgataagc tgtcaaacat gagaattggg taataactga tataattaaa
     8941 ttgaagctct aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt
     9001 tttgctggcc gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct
     9061 ctaccttagc atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc
     9121 ctgtagagac cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat
     9181 ctaaacccac accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt
     9241 ctctttgagc aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac
     9301 ccttagtata ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa
     9361 ggcctctagg ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg
     9421 ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg
     9481 cagagtactg caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta
     9541 aaaaattgta cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat
     9601 cagtcaagat atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta
     9661 actccagtaa ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt
     9721 cgtgcatgat attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct
     9781 tatatgtagc tttcgacatg atttatcttc gtttcctgca tgtttttgtt ctgtgcagtt
     9841 gggttaagaa tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca
     9901 atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa
     9961 aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaaaagct
    10021 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
    10081 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
    10141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
    10201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
    10261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
    10321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
    10381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
    10441 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
    10501 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
    10561 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
    10621 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
    10681 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
    10741 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
    10801 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
    10861 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
    10921 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
    10981 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
    11041 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
    11101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
    11161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    11221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    11281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    11341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    11401 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    11461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    11521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    11581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    11641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    11701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    11761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    11821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    11881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    11941 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    12001 cgcagcgagt cagtgagcga ggaagcggaa ga
//
