LOCUS       sequence-218691-       12203 bp    DNA     circular SYN 14-APR-2025
DEFINITION  TET-inducible expression of c-Kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12203)
  AUTHORS   Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De 
            Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ
  TITLE     Perturbed Wnt signaling leads to neuronal migration delay, altered 
            interhemispheric connections and impaired social behavior.
  JOURNAL   Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w.
  PUBMED    29079819
REFERENCE   2  (bases 1 to 12203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-27"; volume: "8(1):1158. doi"; pages: " 
            10.1038/s41467-017-01046-w"
COMMENT     Sequence Label: pCLXEBR-pTF-cKit
FEATURES             Location/Qualifiers
     source          1..12203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             185..229
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    520..538
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    561..579
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    725..743
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    828..852
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             1522..1530
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(3786..3810)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3847..3863
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3848..3864
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3879..3996
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4053..4264
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4283..4678
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4688..4747
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5810..6181
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    6207..6795
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6260..6280)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6678..6689)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6937..6970)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6990..7170
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7208..7227)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7273..7328
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7327..7346)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7689..7705)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7689..7705)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7702..7724)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7713..7729
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7737..7743,7744..7761,7762..7767))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7782..7803
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7862..8057
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7908..8043
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8063..8197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8100..8119)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8154..8173
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8265..8282)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8436..9024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8516..8535)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9195..9986,9987..10055))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9818..9837
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10056..10160)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        10191..10570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             10572..10829
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10876..11001
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11493..11610
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    11970..12203
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
       61 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
      121 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
      181 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
      241 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
      301 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
      361 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
      421 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
      481 acggtatcgg ttcggtacaa ctcccactag acatatccta ctctatcatt gatagagtga
      541 cccttccata gacatatcct actctatcat tgatagagtg acccttccac taggttcact
      601 tttctctatc attgataggg agtggtaaac aattgatgga caaaatctat caatgataga
      661 gagtgcatgt tactagtatt gttcttctct atcattgata gtgactttgc ctaatcactt
      721 agcaactcta tcattgatag agttctagta ttagcaaggc ctttcctcta tataaggaag
      781 ttcatttcat ttggagaaga tccccgggga tcctctagag tcgaatcaca agtttgtaca
      841 aaaaagcagg cttcaccatg agaggcgctc gcggcgcctg ggatctgctc tgcgtcctgt
      901 tggtcctgct ccgtggccag acagccacgt ctcagccatc tgcaagtcca ggggagccgt
      961 ctccgccatc catccatcca gcacaatcag agttaatagt tgaagctggc gacaccctca
     1021 gcctgacgtg cattgatccc gactttgtca gatggacttt caagacctat ttcaatgaaa
     1081 tggttgagaa taaaaaaaat gaatggatcc aggaaaaggc cgaggccact cgcacgggca
     1141 catacacgtg cagcaacagc aatggcctca cgagttctat ttacgtgttt gttagagatc
     1201 ctgccaaact tttcctggtt ggccttccct tgtttggcaa agaagacagc gacgcgctgg
     1261 tccgctgccc tctgacagac ccacaggtgt ccaattattc cctcatcgag tgtgatggga
     1321 aatctctccc cacggacctg acgtttgtcc caaaccccaa ggctggcatc accatcaaaa
     1381 acgtgaagcg cgcctaccac cggctctgtg tccgctgtgc tgctcagcgt gacggtacat
     1441 ggctgcattc tgacaaattc accctcaaag tgcgggcagc catcagggct atccctgttg
     1501 tgtctgtgcc tgaaacaagt cacctcctta agaaagggga cacatttacg gtggtgtgca
     1561 ccataaaaga tgtgtctaca tccgtgaact ccatgtggct aaagatgaac cctcagcctc
     1621 agcacatagc ccaggtaaag cacaatagct ggcaccgggg tgacttcaat tatgaacgcc
     1681 aggagacgct gactatcagc tcggcaagag ttgacgattc tggagtgttc atgtgttatg
     1741 ccaataatac ttttggatca gcaaatgtca caacaacctt gaaagtagta gaaaaaggat
     1801 tcatcaacat ctcccctgtg aagaacacta cagtatttgt aaccgatgga gaaaacgtag
     1861 atttggttgt tgaatacgag gcctacccca aacccgagca ccagcagtgg atatatatga
     1921 acaggacctc ggctaacaaa gggaaggatt atgtcaaatc tgataacaaa agcaacatca
     1981 gatatgtgaa ccaacttcgc ctgaccagat taaaaggcac agaaggaggc acttatacct
     2041 ttctggtgtc caactctgat gccagtgctt ccgtgacatt caacgtttac gtgaacacaa
     2101 aaccagaaat cctgacgtac gacaggctca taaatggcat gctccagtgt gtggcagagg
     2161 gattcccgga gcccacaata gattggtatt tttgtacagg agcagagcaa agatgtacca
     2221 ctcctgtctc accagtggac gtacaggtcc agaatgtatc tgtgtcacca tttggaaaac
     2281 tggtggttca gagttccata gactccagcg tcttccggca caacggcacg gtggagtgta
     2341 aggcctccaa cgatgtgggc aagagttccg ccttctttaa ctttgcattt aaagagcaaa
     2401 tccaggccca cactctgttc acgccgctgc tcattggctt tgtggttgca gctggcgcga
     2461 tggggatcat tgtgatggtg ctcacctaca aatatttgca gaaacccatg tatgaagtac
     2521 aatggaaggt tgtcgaggag ataaatggaa acaattatgt ttacatagac ccgacgcaac
     2581 ttccttatga tcacaaatgg gagtttccca gaaacaggct gagttttgga aagacattgg
     2641 gagctggtgc cttcgggaag gtcgttgagg ccactgcata tggcttgatt aagtcggatg
     2701 ctgccatgac agttgccgtg aagatgctca aaccaagtgc ccatttaaca gaaagagagg
     2761 ccctaatgtc ggaactgaag gtcctgagct acctgggcaa tcacatgaat attgtgaacc
     2821 tgcttggcgc atgcacggtg ggagggccca ccctggtcat tacagaatat tgttgctatg
     2881 gtgatctttt gaattttttg agaaggaagc gtgactcgtt tattttctca aagcaagaag
     2941 agcaggcaga agcggcactt tataagaacc ttctgcactc aacggagcct tcctgtgaca
     3001 gttcaaatga atatatggac atgaagcctg gcgtttccta cgtggtgcca accaagacag
     3061 acaagaggag atccgcaaga atagactcgt acatagaaag agacgtgact cctgccatca
     3121 tggaagatga cgagctggct ctggacctgg atgatttgct gagcttctcc taccaggtgg
     3181 ccaagggcat ggcgttcctc gcctccaaga attgtattca cagagatttg gcagccagga
     3241 atatcctcct cactcacggg cggatcacaa agatttgcga tttcgggcta gccagagaca
     3301 tcaggaatga ttcgaattac gtggtcaaag gaaatgcacg gctgcccgtg aagtggatgg
     3361 caccagagag cattttcagc tgcgtgtaca catttgaaag tgatgtctgg tcctatggga
     3421 ttttcctctg ggagctcttc tccttaggaa gcagccccta cccagggatg ccggtcgact
     3481 ccaagttcta caagatgacc aaggaaggct tccggatggt cagcccggag cacgcgcctg
     3541 ccgaaatgta tgacgtcatg aagacttgct gggacgctga ccccttgaaa aggccaacat
     3601 tcaagcaggt tgtccaactt attgagaagc agatctcgga cagcaccaag cacatttact
     3661 ccaacttggc aaactgcaac cccaacccag agaaccccgt ggtggtggac cattccgtga
     3721 gggtcaactc ggtgggcagc agcgcctctt ctacgcagcc cctgctcgtg cacgaagatg
     3781 cctgaaccca gctttcttgt acaaagtggt gattcgacct gcaggcatgc aagctcgggc
     3841 cccccctcga ggtcgacggt atcgataagc ttgataactt ttaaaagaaa aggggggatt
     3901 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     3961 gaattacaaa aacaaattac aaaaattcaa aattttatcg atcacgagac tagcctcgag
     4021 aagcttgatc gatggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     4081 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     4141 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     4201 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     4261 acaggtgtcg tgacgcggat ccatggccaa gcctttgtct caagaagaat ccaccctcat
     4321 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     4381 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     4441 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     4501 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     4561 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     4621 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcgg
     4681 atcaggtcaa tgtactaact acgctttgtt gaagcttgcc ggcgatgttg aaagtaaccc
     4741 cggtcctatg tccagactgg acaagagcaa agtcataaac ggcgctctgg aattactcaa
     4801 tggagtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca
     4861 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga
     4921 gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt
     4981 tctgcggaac aacgccaagt cattccgctg tgctctcctc tcacatcgcg acggggctaa
     5041 agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc
     5101 gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg
     5161 ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag
     5221 agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga
     5281 ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga
     5341 gaaacagcta aagtgcgaaa gcggcgggcc ggcctcgagc gaattccagt acctgccaga
     5401 tacagacgat cgtcaccgga ttgaggagaa acgtaaaagg acatatgaga ccttcaagag
     5461 catcatgaag aagagtcctt tcagcggacc caccgacccc cggcctccac ctcgacgcat
     5521 tgctgtgcct tcccgcagct cagcttctgt ccccaagcca gcaccccagc cctatccctt
     5581 tacgtcatcc ctgagcacca tcaactatga tgagtttccc accatggtgt ttccttctgg
     5641 gcagatcagc caggcctcgg ccttggcccc ggcccctccc caagtcctgc cccaggctcc
     5701 agcccctgcc cctgctccag ccatggtatc agctctggcc caggccccag cccctgtccc
     5761 agtcctagcc ccaggccctc ctcaggctgt ggccccacct gcccccaagc ccacccaggc
     5821 tggggaagga acgctgtcag aggccctgct gcagctgcag tttgatgatg aagacctggg
     5881 ggccttgctt ggcaacagca cagacccagc tgtgttcaca gacctggcat ccgtcgacaa
     5941 ctccgagttt cagcagctgc tgaaccaggg catacctgtg gccccccaca caactgagcc
     6001 catgctgatg gagtaccctg aggctataac tcgcctagtg acaggggccc agaggccccc
     6061 cgacccagct cctgctccac tgggggcccc ggggctcccc aatggcctcc tttcaggaga
     6121 tgaagacttc tcctccattg cggacatgga cttctcagcc ctgctgagtc agatcagctc
     6181 ctaaagatcc gcgtcgtacg gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     6241 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6301 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6361 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6421 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6481 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6541 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6601 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6661 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6721 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6781 gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc
     6841 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6901 ccaacgaaga caagatggga tcaattcacc atgggaataa cttcgtatag catacattat
     6961 acgaagttat gctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7021 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7081 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7141 ccttttagtc agtgtggaaa atctctagca gcatctagtt gaatcgccag tgtgtcgagc
     7201 tagcagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     7261 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     7321 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     7381 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     7441 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     7501 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     7561 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     7621 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg
     7681 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7741 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7801 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     7861 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     7921 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7981 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8041 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
     8101 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8161 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
     8221 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8281 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8341 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8401 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8461 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8521 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8581 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8641 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8701 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8761 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8821 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8881 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8941 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9001 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9061 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9121 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9181 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9241 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9301 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9361 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9421 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9481 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9541 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9601 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9661 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9721 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9781 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9841 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9901 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9961 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10021 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10081 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10141 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc gacattgatt
    10201 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
    10261 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
    10321 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
    10381 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
    10441 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
    10501 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
    10561 tattaccatg ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
    10621 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
    10681 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    10741 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    10801 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
    10861 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
    10921 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
    10981 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
    11041 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
    11101 agggagctag aacgattcgc agttaatact ggcctgttag aaacatcaga aggctgtaga
    11161 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    11221 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
    11281 gaagctttag acaagataga ggaagagcaa aacaaaagta agaaaaaagc acagcaagca
    11341 gcaggatctt cagacctgga aattccctac aatccccaaa gtcaaggagt agtagaatct
    11401 atgaataaag aattaaagaa aattatagga caggtaagag atcaggctga acatcttaag
    11461 acagcagtac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
    11521 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
    11581 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagaaatcca
    11641 ctttggaaag gaccagcaaa gctcctctgg aaaggtgaag gggcagtagt aatacaagat
    11701 aatagtgaca taaaagtagt gccaagaaga aaagcaaaga tcattaggga ttatggaaaa
    11761 cagatggcag gtgatgattg tgtggcaagt agacaggatg aggattagaa catggaaaag
    11821 tttagtaaaa caccataagg aggagatatg agggacaatt ggagaagtga attatataaa
    11881 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    11941 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    12001 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    12061 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    12121 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    12181 tacctaaagg atcaacagct cct
//
