LOCUS       sequence-218771-         525 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Encodes codon optimized 3xFLAG-SIVSAB Vpr (HQ378594) and puromycin 
            resistance protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, 
            Diehl WE, Luban J
  TITLE     Primate immunodeficiency virus proteins Vpx and Vpr counteract 
            transcriptional repression of proviruses by the HUSH complex.
  JOURNAL   Nat Microbiol. 2018 Dec;3(12):1354-1361. doi: 
            10.1038/s41564-018-0256-x. Epub 2018 Oct 8.
  PUBMED    30297740
REFERENCE   2  (bases 1 to 525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-018-0256-x"; journalName: "Nat Microbiol"; date: 
            "2018-12"; volume: "3"; issue: "12"; pages: "1354-1361"
COMMENT     Sequence Label: pscALPS-SIV-SAB-92018 vpr
FEATURES             Location/Qualifiers
     source          1..525
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..87
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 tctagagccg ccatggatta taaagatgac gatgacaaag actataaaga cgatgacgac
       61 aaagattaca aggacgacga cgacaaagcg gccgctatgg cctcaggggg gtggcttccc
      121 ccgacaggag gggacccccc ccaggacccc cccaagaacc caagggagga aattcctggg
      181 tggttggaaa cgtgggattt gccacgagaa ccgttcgatg aatggctgag agatatgctc
      241 caggacctca acgcggaagc acaatgtcat ttcccccgga accttctctt tcggctttgg
      301 tggaatatcg tggaggagcc ggctattgac cgaggtcaac ccaggctgga gggatggtac
      361 aaatactata ttatagtaca acgggcgctt ttcgttcata tgaagggtcg atgctgtaaa
      421 ccaaagaccc atccagccta cggaccaggt agaggacctc cgggtctcgg gggtgctcct
      481 ggaggagcag cagcggctcc gccaggcttg taacccgggg aattc
//
