LOCUS       sequence-218868-        8852 bp    DNA     circular SYN 14-APR-2025
DEFINITION  3rd generation lentiviral vector. eFFly luciferase for in vivo 
            Imaging, mCherry for fluorescent detection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Ebinger S, Ozdemir EZ, Ziegenhain C, Tiedt S, Castro Alves C, 
            Grunert M, Dworzak M, Lutz C, Turati VA, Enver T, Horny HP, Sotlar 
            K, Parekh S, Spiekermann K, Hiddemann W, Schepers A, Polzer B, 
            Kirsch S, Hoffmann M, Knapp B, Hasenauer J, Pfeifer H, 
            Panzer-Grumayer R, Enard W, Gires O, Jeremias I
  TITLE     Characterization of Rare, Dormant, and Therapy-Resistant Cells in 
            Acute Lymphoblastic Leukemia.
  JOURNAL   Cancer Cell. 2016 Dec 12;30(6):849-862. doi: 
            10.1016/j.ccell.2016.11.002. Epub 2016 Dec 1.
  PUBMED    27916615
REFERENCE   2  (bases 1 to 8852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.11.002"; journalName: "Cancer Cell"; date: 
            "2016-12-12- 12"; volume: "30"; issue: "6"; pages: "849-862"
COMMENT     Sequence Label: pCDH-EF1a-eFFly-mCherry
FEATURES             Location/Qualifiers
     source          1..8852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    169..285
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        328..539
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             552..820
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2516..2569
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2576..3286
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2721..2739)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2989..3008
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3293..3881
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3346..3366)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3764..3775)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3955..4188
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4260..4381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4297..4316)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4351..4370
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4421..4556
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4589..4605)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4589..4605)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4602..4624)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4613..4629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4637..4643,4644..4661,4662..4667))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4682..4703
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4820..4837)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4991..5579)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(5750..6541,6542..6610))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6373..6392
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6611..6715)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6783..6801
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6839..6861)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6961..6980
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7174..7196
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7188..7205
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7189..7205
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7220..7446
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7447..7627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7674..7799
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8292..8525
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8710..8754
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
       61 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
      121 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
      181 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
      241 acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga tactagtgga
      301 tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
      361 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact
      421 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
      481 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc
      541 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc
      601 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt
      661 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt
      721 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct
      781 acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta
      841 ctctagagct agcgaattca tggaagatgc caagaacatc aagaaaggcc ctgccccctt
      901 ctaccccctg gaagatggca cagccggcga gcagctgcac aaggccatga agagatacgc
      961 cctggtgccc ggcaccatcg ccttcaccga cgcccacatc gaggtggaca tcacctacgc
     1021 cgagtatttc gagatgagcg tgcggctggc cgaggccatg aaacgctacg gcctgaacac
     1081 caaccaccgg atcgtggtgt gcagcgagaa cagcctgcag ttcttcatgc ccgtgctggg
     1141 cgccctgttc atcggcgtgg ccgtggcccc tgccaacgac atctacaacg agcgggagct
     1201 gctgaacagc atgggcatca gccagcccac cgtggtgttc gtgagcaaga agggcctgca
     1261 gaaaatcctg aacgtgcaga agaagctgcc catcatccag aaaatcatca tcatggacag
     1321 caagaccgac taccagggct tccagagcat gtacaccttc gtgaccagcc acctgccccc
     1381 tggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaga ccatcgccct
     1441 gatcatgaac agcagcggca gcaccggcct gcctaaaggc gtggccctgc ctcaccggac
     1501 cgcctgcgtg cggttcagcc acgcccggga ccccatcttc ggcaaccaga tcatccccga
     1561 caccgccatc ctgagcgtgg tgcccttcca ccacggcttc ggcatgttca ccaccctggg
     1621 ctacctgatc tgcggcttcc gggtggtgct gatgtaccgg ttcgaggaag agctgttcct
     1681 gcggagcctg caggactaca agatccagag cgccctgctg gtgcccaccc tgttcagctt
     1741 tttcgccaag agcaccctga tcgacaagta cgacctgagc aacctgcacg agatcgccag
     1801 cggcggagcc cccctgtcca aagaagtggg cgaagccgtc gccaagcggt tccacctgcc
     1861 cggcatccgg cagggctatg gcctgaccga gaccacaagc gccattctga tcacccccga
     1921 gggcgacgac aagcctggcg ccgtgggcaa ggtggtgcct ttcttcgagg ccaaggtggt
     1981 ggacctggac accggcaaga ccctgggcgt gaaccagcgg ggcgagctgt gcgtgagggg
     2041 ccccatgatc atgagcggct acgtgaacaa ccccgaggcc accaacgccc tgattgacaa
     2101 ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc acttcttcat
     2161 cgtggaccgg ctgaagagcc tgatcaagta caagggctac caggtggccc cagccgagct
     2221 ggaaagcatc ctgctgcagc accccaacat cttcgatgcc ggggtggccg gactgcccga
     2281 cgacgatgcc ggcgagctgc ctgccgccgt ggtggtgctg gaacacggca aaaccatgac
     2341 cgagaaagaa atcgtggact acgtggccag ccaggtgacc accgccaaga aactgagagg
     2401 cggcgtggtg tttgtggacg aggtgcccaa gggcctgaca ggcaagctgg acgcccggaa
     2461 gatccgggag atcctgatca aggccaagaa gggcggcaag ggatccgcgg ccgctgaggg
     2521 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta tgcatatggt
     2581 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     2641 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     2701 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     2761 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     2821 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     2881 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     2941 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     3001 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     3061 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     3121 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     3181 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     3241 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaagtcg acaatcaacc
     3301 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3361 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3421 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     3481 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     3541 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     3601 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     3661 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     3721 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     3781 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     3841 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa
     3901 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     3961 ggctaattca ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt
     4021 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4081 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4141 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     4201 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     4261 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4321 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4381 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     4441 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4501 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga
     4561 cttttgcaga gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc
     4621 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     4681 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     4741 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     4801 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     4861 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     4921 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     4981 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5041 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5101 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5161 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     5221 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5281 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     5341 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     5401 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     5461 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5521 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5581 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5641 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     5701 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     5761 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     5821 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     5881 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     5941 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6001 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6061 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6121 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     6181 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     6241 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     6301 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     6361 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     6421 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     6481 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     6541 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     6601 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     6661 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     6721 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     6781 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     6841 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     6901 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     6961 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     7021 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     7081 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     7141 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     7201 ccagtgccaa gctgacgcgt gtagtcttat gcaatactct tgtagtcttg caacatggta
     7261 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     7321 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     7381 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     7441 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7501 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7561 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7621 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     7681 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     7741 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     7801 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     7861 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     7921 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     7981 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     8041 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     8101 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cactgatctt cagacctgga
     8161 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     8221 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     8281 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     8341 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     8401 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     8461 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     8521 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     8581 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     8641 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     8701 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     8761 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     8821 ttggtaggtt taagaatagt ttttgctgta ct
//
