LOCUS       sequence-218874-       11947 bp    DNA     circular SYN 14-APR-2025
DEFINITION  TET-inducible expression of a constitutively active form of the 
            BetaCatenin protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11947)
  AUTHORS   Boitard M, Bocchi R, Egervari K, Petrenko V, Viale B, Gremaud S, 
            Zgraggen E, Salmon P, Kiss JZ
  TITLE     Wnt signaling regulates multipolar-to-bipolar transition of 
            migrating neurons in the cerebral cortex.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1349-61. doi: 
            10.1016/j.celrep.2015.01.061. Epub 2015 Feb 26.
  PUBMED    25732825
REFERENCE   2  (bases 1 to 11947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.061"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1349-61"
COMMENT     Sequence Label: pCWXPGR-pTF-betaCatenin
FEATURES             Location/Qualifiers
     source          1..11947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    165..183
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    245..269
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             278..301
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(2525..2549)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2566..3076
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2995..3013
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      3092..3101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3098..3100,3101..3103,3104..3814)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3098..3100,3101..3103,3104..3814)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3143..3164)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3404..3423)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3751..3772
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3830..3889
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             4958..5329
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    5355..5943
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5408..5428)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5826..5837)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5946..5962)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5947..5963)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(6091..6124)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6144..6324
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6362..6381)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6427..6482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6481..6500)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6843..6859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6843..6859)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6856..6878)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6867..6883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6891..6897,6898..6915,6916..6921))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6936..6957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7016..7211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7062..7197
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7217..7351
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7254..7273)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7308..7327
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7419..7436)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7590..8177)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7670..7689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8348..9139,9140..9208))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8971..8990
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9209..9313)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9344..9723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             9725..9982
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10029..10154
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10646..10763
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    11123..11356
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11541..11585
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    11860..11880
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    11907..11925
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 actctatcat tgatagagtg acccttccac taggttcact tttctctatc attgataggg
       61 agtggtaaac aattgatgga caaaatctat caatgataga gagtgcatgt tactagtatt
      121 gttcttctct atcattgata gtgactttgc ctaatcactt agcaactcta tcattgatag
      181 agttctagta ttagcaaggc ctttcctcta tataaggaag ttcatttcat ttggagaaga
      241 tcccccaagt ttgtacaaaa aagcaggctt caccatggac tacaaagacg atgacgacaa
      301 gcttgcggcc gctctgagtg gtaaaggcaa tcctgaggaa gaggatgtgg atacctccca
      361 agtcctgtat gagtgggaac agggattttc tcagtccttc actcaagaac aagtagctga
      421 tattgatgga cagtatgcaa tgactcgagc tcagagggta cgagctgcta tgttccctga
      481 gacattagat gagggcatgc agatcccatc tacacagttt gatgctgctc atcccactaa
      541 tgtccagcgt ttggctgaac catcacagat gctgaaacat gcagttgtaa acttgattaa
      601 ctatcaagat gatgcagaac ttgccacacg ggcaatccct gaactgacaa aactgctaaa
      661 tgacgaggac caggtggtgg ttaataaggc tgcagttatg gtccatcagc tttctaaaaa
      721 ggaagcttcc agacacgcta tcatgcgttc tcctcagatg gtgtctgcta ttgtacgtac
      781 catgcagaat acaaatgatg tagaaacagc tcgttgtacc gctgggacct tgcataacct
      841 ttcccatcat cgtgagggct tactggccat ctttaagtct ggaggcattc ctgccctggt
      901 gaaaatgctt ggttcaccag tggattctgt gttgttttat gccattacaa ctctccacaa
      961 ccttttatta catcaagaag gagctaaaat ggcagtgcgt ttagctggtg ggctgcagaa
     1021 aatggttgcc ttgctcaaca aaacaaatgt taaattcttg gctattacga cagactgcct
     1081 tcaaatttta gcttatggca accaagaaag caagctcatc atactggcta gtggtggacc
     1141 ccaagcttta gtaaatataa tgaggaccta tacttacgaa aaactactgt ggaccacaag
     1201 cagagtgctg aaggtgctat ctgtctgctc tagtaataag ccggctattg tagaagctgg
     1261 tggaatgcaa gctttaggac ttcacctgac agatccaagt caacgtcttg ttcagaactg
     1321 tctttggact ctcaggaatc tttcagatgc tgcaactaaa caggaaggga tggaaggtct
     1381 ccttgggact cttgttcagc ttctgggttc agatgatata aatgtggtca cctgtgcagc
     1441 tggaattctt tctaacctca cttgcaataa ttataagaac aagatgatgg tctgccaagt
     1501 gggtggtata gaggctcttg tgcgtactgt ccttcgggct ggtgacaggg aagacatcac
     1561 tgagcctgcc atctgtgctc ttcgtcatct gaccagccga caccaagaag cagagatggc
     1621 ccagaatgca gttcgccttc actatggact accagttgtg gttaagctct tacacccacc
     1681 atcccactgg cctctgataa aggctactgt tggattgatt cgaaatcttg ccctttgtcc
     1741 cgcaaatcat gcacctttgc gtgagcaggg tgccattcca cgactagttc agttgcttgt
     1801 tcgtgcacat caggataccc agcgccgtac gtccatgggt gggacacagc agcaatttgt
     1861 ggagggggtc cgcatggaag aaatagttga aggttgtacc ggagcccttc acatcctagc
     1921 tcgggatgtt cacaaccgaa ttgttatcag aggactaaat accattccat tgtttgtgca
     1981 gctgctttat tctcccattg aaaacatcca aagagtagct gcaggggtcc tctgtgaact
     2041 tgctcaggac aaggaagctg cagaagctat tgaagctgag ggagccacag ctcctctgac
     2101 agagttactt cactctagga atgaaggtgt ggcgacatat gcagctgctg ttttgttccg
     2161 aatgtctgag gacaagccac aagattacaa gaaacggctt tcagttgagc tgaccagctc
     2221 tctcttcaga acagagccaa tggcttggaa tgagactgct gatcttggac ttgatattgg
     2281 tgcccaggga gaaccccttg gatatcgcca ggatgatcct agctatcgtt cttttcactc
     2341 tggtggatat ggccaggatg ccttgggtat ggaccccatg atggaacatg agatgggtgg
     2401 ccaccaccct ggtgctgact atccagttga tgggctgcca gatctggggc atgcccagga
     2461 cctcatggat gggctgcctc caggtgacag caatcagctg gcctggtttg atactgacct
     2521 gtaaacccag ctttcttgta caaagtggtg ataatcgaat tccacggggt tggggttgcg
     2581 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     2641 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     2701 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     2761 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     2821 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     2881 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     2941 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     3001 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     3061 cgacctctct ccccaggggg atccaccggt cgccaccatg gtgagcaagg gcgaggagct
     3121 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3181 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     3241 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     3301 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3361 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     3421 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3481 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     3541 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     3601 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     3661 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     3721 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     3781 cgggatcact ctcggcatgg acgagctgta caaggctagc ggatcaggtc aatgtactaa
     3841 ctacgctttg ttgaagcttg ccggcgatgt tgaaagtaac cccggtcctg gatccatgtc
     3901 cagactggac aagagcaaag tcataaacgg cgctctggaa ttactcaatg gagtcggtat
     3961 cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta
     4021 ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag
     4081 gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa
     4141 cgccaagtca ttccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg
     4201 cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca
     4261 gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact
     4321 gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac
     4381 caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc
     4441 cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa
     4501 gtgcgaaagc ggcgggccgg cctcgagcga attccagtac ctgccagata cagacgatcg
     4561 tcaccggatt gaggagaaac gtaaaaggac atatgagacc ttcaagagca tcatgaagaa
     4621 gagtcctttc agcggaccca ccgacccccg gcctccacct cgacgcattg ctgtgccttc
     4681 ccgcagctca gcttctgtcc ccaagccagc accccagccc tatcccttta cgtcatccct
     4741 gagcaccatc aactatgatg agtttcccac catggtgttt ccttctgggc agatcagcca
     4801 ggcctcggcc ttggccccgg cccctcccca agtcctgccc caggctccag cccctgcccc
     4861 tgctccagcc atggtatcag ctctggccca ggccccagcc cctgtcccag tcctagcccc
     4921 aggccctcct caggctgtgg ccccacctgc ccccaagccc acccaggctg gggaaggaac
     4981 gctgtcagag gccctgctgc agctgcagtt tgatgatgaa gacctggggg ccttgcttgg
     5041 caacagcaca gacccagctg tgttcacaga cctggcatcc gtcgacaact ccgagtttca
     5101 gcagctgctg aaccagggca tacctgtggc cccccacaca actgagccca tgctgatgga
     5161 gtaccctgag gctataactc gcctagtgac aggggcccag aggccccccg acccagctcc
     5221 tgctccactg ggggccccgg ggctccccaa tggcctcctt tcaggagatg aagacttctc
     5281 ctccattgcg gacatggact tctcagccct gctgagtcag atcagctcct aaagatctga
     5341 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5401 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     5461 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5521 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5581 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5641 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5701 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     5761 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5821 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5881 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5941 cgcatcgata ccgtcgacct cgaggttcgc tttaagacca atgacttaca aggcagctgt
     6001 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6061 aagacaagat gggatcaatt caccatggga ataacttcgt atagcataca ttatacgaag
     6121 ttatgctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6181 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6241 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6301 agtcagtgtg gaaaatctct agcagcatct agttgaatcg ccagtgtgtc gagctagcag
     6361 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
     6421 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     6481 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
     6541 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
     6601 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
     6661 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
     6721 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
     6781 tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca
     6841 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6901 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6961 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc
     7021 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7081 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7141 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7201 gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7261 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7321 actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg
     7381 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7441 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7501 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7561 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7621 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7681 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttacggat
     7741 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7801 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7861 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7921 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7981 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8041 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8101 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8161 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8221 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8281 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8341 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8401 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8461 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8521 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8581 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8641 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8701 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8761 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8821 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8881 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8941 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9001 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9061 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9121 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9181 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9241 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9301 taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg attattgact
     9361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     9421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     9481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     9541 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     9601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     9661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     9721 atgggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     9781 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     9841 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     9901 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     9961 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
    10021 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
    10081 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
    10141 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
    10201 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
    10261 tagaacgatt cgcagttaat actggcctgt tagaaacatc agaaggctgt agacaaatac
    10321 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
    10381 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
    10441 tagacaagat agaggaagag caaaacaaaa gtaagaaaaa agcacagcaa gcagcaggat
    10501 cttcagacct ggaaattccc tacaatcccc aaagtcaagg agtagtagaa tctatgaata
    10561 aagaattaaa gaaaattata ggacaggtaa gagatcaggc tgaacatctt aagacagcag
    10621 tacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
    10681 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    10741 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagaaat ccactttgga
    10801 aaggaccagc aaagctcctc tggaaaggtg aaggggcagt agtaatacaa gataatagtg
    10861 acataaaagt agtgccaaga agaaaagcaa agatcattag ggattatgga aaacagatgg
    10921 caggtgatga ttgtgtggca agtagacagg atgaggatta gaacatggaa aagtttagta
    10981 aaacaccata aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
    11041 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
    11101 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
    11161 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
    11221 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
    11281 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
    11341 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
    11401 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
    11461 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
    11521 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
    11581 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
    11641 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
    11701 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
    11761 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
    11821 tagtgaacgg atctcgacgg tatctagtga ttatcggatc aactttgtat agaaaagttg
    11881 ggtacaactc ccactagaca tatcctactc tatcattgat agagtgaccc ttccatagac
    11941 atatcct
//
