LOCUS       sequence-219868-        8727 bp    DNA     circular SYN 14-APR-2025
DEFINITION  expresses ABE7.9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu
            DR
  TITLE     Programmable base editing of A*T to G*C in genomic DNA without DNA 
            cleavage.
  JOURNAL   Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. 
            Epub 2017 Oct 25.
  PUBMED    29160308
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature24644"; journalName: "Nature"; date: "2017-11-23- 
            23"; volume: "551"; issue: "7681"; pages: "464-471"
COMMENT     Sequence Label: pCMV-ABE7.9
FEATURES             Location/Qualifiers
     source          1..8727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(50..69)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        241..620
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        621..824
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     775..795
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     866..885
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        866..884
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(898..1398,1399..1494,1495..1992,1993..2088)
                     /codon_start=1
                     /product="adenine base editor comprising wild-type E. coli 
                     TadA fused to the evolved TadA*(7.9) mutant (Gaudelli et 
                     al., 2017)"
                     /label=ABE(7.9)
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGW
                     NRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVF
                     GARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQ
                     SSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGSSEVEFSHEYWMRHALTLAKRALD
                     EREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVT
                     FEPCVMCAGAMIHSRIGRVVFGVRNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECN
                     ALLCYFFRMPRQVFNAQKKAQSSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             join(2089..5487,5488..5490,5491..6087,6088,6089..6091,
                     6092..6093,6094..6096,6097..6189)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(2089..2112,2113..2115,2116..6189)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6202..6222
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6231..6248
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6271..6288)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6277..6501
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6572..6588)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6572..6588)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6585..6607)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6596..6612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6620..6626,6627..6644,6645..6650))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6665..6686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6803..6820)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6974..7562)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7054..7073)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7733..8524,8525..8593))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8356..8375
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8594..8698)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct
       61 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
      121 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
      181 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
      241 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      481 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      541 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      841 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      901 tccgaagtcg agttttccca tgagtactgg atgagacacg cattgactct cgcaaagagg
      961 gcttgggatg aacgcgaggt gcccgtgggg gcagtactcg tgcataacaa tcgcgtaatc
     1021 ggcgaaggtt ggaataggcc gatcggacgc cacgacccca ctgcacatgc ggaaatcatg
     1081 gcccttcgac agggagggct tgtgatgcag aattatcgac ttatcgatgc gacgctgtac
     1141 gtcacgcttg aaccttgcgt aatgtgcgcg ggagctatga ttcactcccg cattggacga
     1201 gttgtattcg gtgcccgcga cgccaagacg ggtgccgcag gttcactgat ggacgtgctg
     1261 catcacccag gcatgaacca ccgggtagaa atcacagaag gcatattggc ggacgaatgt
     1321 gcggcgctgt tgtccgactt ttttcgcatg cggaggcagg agatcaaggc ccagaaaaaa
     1381 gcacaatcct ctactgactc tggtggttct tctggtggtt ctagcggcag cgagactccc
     1441 gggacctcag agtccgccac acccgaaagt tctggtggtt cttctggtgg ttcttccgaa
     1501 gtcgagtttt cccatgagta ctggatgaga cacgcattga ctctcgcaaa gagggctctc
     1561 gatgaacgcg aggtgcccgt gggggcagta ctcgtgctca acaatcgcgt aatcggcgaa
     1621 ggttggaata gggcaatcgg actccacgac cccactgcac atgcggaaat catggccctt
     1681 cgacagggag ggcttgtgat gcagaattat cgacttatcg atgcgacgct gtacgtcacg
     1741 tttgaacctt gcgtaatgtg cgcgggagct atgattcact cccgcattgg acgagttgta
     1801 ttcggtgttc gcaacgccaa gacgggtgcc gcaggttcac tgatggacgt gctgcattac
     1861 ccaggcatga accaccgggt agaaatcaca gaaggcatat tggcggacga atgtaacgcg
     1921 ctgttgtgtt acttttttcg catgcccagg caggtcttta acgcccagaa aaaagcacaa
     1981 tcctctactg actctggtgg ttcttctggt ggttctagcg gcagcgagac tcccgggacc
     2041 tcagagtccg ccacacccga aagttctggt ggttcttctg gtggttctga taaaaagtat
     2101 tctattggtt tagccatcgg cactaattcc gttggatggg ctgtcataac cgatgaatac
     2161 aaagtacctt caaagaaatt taaggtgttg gggaacacag accgtcattc gattaaaaag
     2221 aatcttatcg gtgccctcct attcgatagt ggcgaaacgg cagaggcgac tcgcctgaaa
     2281 cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa tatgttactt acaagaaatt
     2341 tttagcaatg agatggccaa agttgacgat tctttctttc accgtttgga agagtccttc
     2401 cttgtcgaag aggacaagaa acatgaacgg caccccatct ttggaaacat agtagatgag
     2461 gtggcatatc atgaaaagta cccaacgatt tatcacctca gaaaaaagct agttgactca
     2521 actgataaag cggacctgag gttaatctac ttggctcttg cccatatgat aaagttccgt
     2581 gggcactttc tcattgaggg tgatctaaat ccggacaact cggatgtcga caaactgttc
     2641 atccagttag tacaaaccta taatcagttg tttgaagaga accctataaa tgcaagtggc
     2701 gtggatgcga aggctattct tagcgcccgc ctctctaaat cccgacggct agaaaacctg
     2761 atcgcacaat tacccggaga gaagaaaaat gggttgttcg gtaaccttat agcgctctca
     2821 ctaggcctga caccaaattt taagtcgaac ttcgacttag ctgaagatgc caaattgcag
     2881 cttagtaagg acacgtacga tgacgatctc gacaatctac tggcacaaat tggagatcag
     2941 tatgcggact tatttttggc tgccaaaaac cttagcgatg caatcctcct atctgacata
     3001 ctgagagtta atactgagat taccaaggcg ccgttatccg cttcaatgat caaaaggtac
     3061 gatgaacatc accaagactt gacacttctc aaggccctag tccgtcagca actgcctgag
     3121 aaatataagg aaatattctt tgatcagtcg aaaaacgggt acgcaggtta tattgacggc
     3181 ggagcgagtc aagaggaatt ctacaagttt atcaaaccca tattagagaa gatggatggg
     3241 acggaagagt tgcttgtaaa actcaatcgc gaagatctac tgcgaaagca gcggactttc
     3301 gacaacggta gcattccaca tcaaatccac ttaggcgaat tgcatgctat acttagaagg
     3361 caggaggatt tttatccgtt cctcaaagac aatcgtgaaa agattgagaa aatcctaacc
     3421 tttcgcatac cttactatgt gggacccctg gcccgaggga actctcggtt cgcatggatg
     3481 acaagaaagt ccgaagaaac gattactcca tggaattttg aggaagttgt cgataaaggt
     3541 gcgtcagctc aatcgttcat cgagaggatg accaactttg acaagaattt accgaacgaa
     3601 aaagtattgc ctaagcacag tttactttac gagtatttca cagtgtacaa tgaactcacg
     3661 aaagttaagt atgtcactga gggcatgcgt aaacccgcct ttctaagcgg agaacagaag
     3721 aaagcaatag tagatctgtt attcaagacc aaccgcaaag tgacagttaa gcaattgaaa
     3781 gaggactact ttaagaaaat tgaatgcttc gattctgtcg agatctccgg ggtagaagat
     3841 cgatttaatg cgtcacttgg tacgtatcat gacctcctaa agataattaa agataaggac
     3901 ttcctggata acgaagagaa tgaagatatc ttagaagata tagtgttgac tcttaccctc
     3961 tttgaagatc gggaaatgat tgaggaaaga ctaaaaacat acgctcacct gttcgacgat
     4021 aaggttatga aacagttaaa gaggcgtcgc tatacgggct ggggacgatt gtcgcggaaa
     4081 cttatcaacg ggataagaga caagcaaagt ggtaaaacta ttctcgattt tctaaagagc
     4141 gacggcttcg ccaataggaa ctttatgcag ctgatccatg atgactcttt aaccttcaaa
     4201 gaggatatac aaaaggcaca ggtttccgga caaggggact cattgcacga acatattgcg
     4261 aatcttgctg gttcgccagc catcaaaaag ggcatactcc agacagtcaa agtagtggat
     4321 gagctagtta aggtcatggg acgtcacaaa ccggaaaaca ttgtaatcga gatggcacgc
     4381 gaaaatcaaa cgactcagaa ggggcaaaaa aacagtcgag agcggatgaa gagaatagaa
     4441 gagggtatta aagaactggg cagccagatc ttaaaggagc atcctgtgga aaatacccaa
     4501 ttgcagaacg agaaacttta cctctattac ctacaaaatg gaagggacat gtatgttgat
     4561 caggaactgg acataaaccg tttatctgat tacgacgtcg atcacattgt accccaatcc
     4621 tttttgaagg acgattcaat cgacaataaa gtgcttacac gctcggataa gaaccgaggg
     4681 aaaagtgaca atgttccaag cgaggaagtc gtaaagaaaa tgaagaacta ttggcggcag
     4741 ctcctaaatg cgaaactgat aacgcaaaga aagttcgata acttaactaa agctgagagg
     4801 ggtggcttgt ctgaacttga caaggccgga tttattaaac gtcagctcgt ggaaacccgc
     4861 caaatcacaa agcatgttgc acagatacta gattcccgaa tgaatacgaa atacgacgag
     4921 aacgataagc tgattcggga agtcaaagta atcactttaa agtcaaaatt ggtgtcggac
     4981 ttcagaaagg attttcaatt ctataaagtt agggagataa ataactacca ccatgcgcac
     5041 gacgcttatc ttaatgccgt cgtagggacc gcactcatta agaaataccc gaagctagaa
     5101 agtgagtttg tgtatggtga ttacaaagtt tatgacgtcc gtaagatgat cgcgaaaagc
     5161 gaacaggaga taggcaaggc tacagccaaa tacttctttt attctaacat tatgaatttc
     5221 tttaagacgg aaatcactct ggcaaacgga gagatacgca aacgaccttt aattgaaacc
     5281 aatggggaga caggtgaaat cgtatgggat aagggccggg acttcgcgac ggtgagaaaa
     5341 gttttgtcca tgccccaagt caacatagta aagaaaactg aggtgcagac cggagggttt
     5401 tcaaaggaat cgattcttcc aaaaaggaat agtgataagc tcatcgctcg taaaaaggac
     5461 tgggacccga aaaagtacgg tggcttcgat agccctacag ttgcctattc tgtcctagta
     5521 gtggcaaaag ttgagaaggg aaaatccaag aaactgaagt cagtcaaaga attattgggg
     5581 ataacgatta tggagcgctc gtcttttgaa aagaacccca tcgacttcct tgaggcgaaa
     5641 ggttacaagg aagtaaaaaa ggatctcata attaaactac caaagtatag tctgtttgag
     5701 ttagaaaatg gccgaaaacg gatgttggct agcgccggag agcttcaaaa ggggaacgaa
     5761 ctcgcactac cgtctaaata cgtgaatttc ctgtatttag cgtcccatta cgagaagttg
     5821 aaaggttcac ctgaagataa cgaacagaag caactttttg ttgagcagca caaacattat
     5881 ctcgacgaaa tcatagagca aatttcggaa ttcagtaaga gagtcatcct agctgatgcc
     5941 aatctggaca aagtattaag cgcatacaac aagcacaggg ataaacccat acgtgagcag
     6001 gcggaaaata ttatccattt gtttactctt accaacctcg gcgctccagc cgcattcaag
     6061 tattttgaca caacgataga tcgcaaacga tacacttcta ccaaggaggt gctagacgcg
     6121 acactgattc accaatccat cacgggatta tatgaaactc ggatagattt gtcacagctt
     6181 gggggtgact ctggtggttc tcccaagaag aagaggaaag tctaaccggt catcatcacc
     6241 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     6301 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     6361 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     6421 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     6481 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgataccgt
     6541 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6601 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctagggtg
     6661 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6721 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6781 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6841 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6901 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6961 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7141 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7381 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7501 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7561 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7621 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7681 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7741 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7801 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7861 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7921 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7981 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8041 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8101 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8161 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8221 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8281 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8341 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8401 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8461 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8521 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8581 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8641 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8701 catttccccg aaaagtgcca cctgacg
//
