LOCUS       sequence-219999-       11120 bp    DNA     circular SYN 14-APR-2025
DEFINITION  destination vector for GreenGate cloning method, contains 2x a set 
            of modules from A-F, ""Driver line"", tissues-specific promoter, 
            Dex-inducible mTurquoise2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 11120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pSW648 - pREV>>
FEATURES             Location/Qualifiers
     source          1..11120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             4658..5002
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     terminator      5009..5650
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     protein_bind    5756..5775
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    5808..5827
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    5860..5879
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    5912..5931
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        5955..6001
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             join(6086..6088,6089..6091,6092..6802)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6131..6152)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6392..6411)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6739..6760
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        7466..7846
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    9139..9163
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(9254..9842)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9334..9353)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10013..10801)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(11092..11120,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt ctcgaactag gctcggacga agtaagcggc cgcaacctta
      601 attaaacacg gcgcgccaac agcgatcgca ccaggtacca cctacacctc tttctgatta
      661 ctagcccact ctttggtggt tcttacatat cacacctaac tatactgtgt atccttgaag
      721 tgaaaatcaa atttaccatt cgtatcttac ttacatacac tattattttt cctttttttt
      781 ttcactcaag gtcctactct ttgataccat agctataatt tggaaataac tatttacagt
      841 gtattaatta tacctaaaca gtttaatctg gactaaatat ttagatagat gttacaaatt
      901 tggttcgtct aataaatgaa gacaagacat gctaacaaat aaaacactac cacaaaggga
      961 tagtgagaga atgtgttttg caacaagaca taactttgat tgcttgatgt gttaaaatga
     1021 ttatgtcaga gacagagagc gatcataggc tttctttatt tctaataacg tcgattttct
     1081 tcttcttttc tggcgttcaa taatgtcgaa ttttaattct tgatttttgc aactctaaat
     1141 atccttaacg acattgaagc atggtgcatg tcgatcgtta ataataaagt tgaacaaaaa
     1201 tcttgttgaa ttaattacaa gcacagcttc aatagcataa ctttacgaga cgaccagatc
     1261 ttatagacga gtttcgcttt tactttttta atgattaaaa ctttcatcgg agaacataag
     1321 tcttcctctt aattaaaatt actacccgtg cataacttca ttttttaaaa catcaataat
     1381 taatatacga ttacaaccct aaaaattagt caccctatag tacataacaa tgatgatagt
     1441 tttttctttt tggtgtaatg ttaaaataaa atgttagcca tattaaacga tagttctttt
     1501 aacttagcca ttgtaagata tttcttactt tagtttttcc gtagaagata ttcattatgg
     1561 tatggatagt atatacctta actgagttta aatattggat caataccatc taataacaca
     1621 tatctgatgt tcaatactta ataattttgc ataaatgtta agcgtgacaa cttaaaaaaa
     1681 aacacatcaa cagagtaaaa acatatctgt taaataagaa aaatgtcatt tttataacac
     1741 ttaaaaaaaa atgcatgaag cgtttcatag tttttttttt tttcaaagta atgtaggcgt
     1801 tagatatttc ttacaatttt ttgaaaaata ttttttatgt tgtgattggc tgatatcagg
     1861 taactaaaac ttctttaaag aattgaagaa aatttgaaaa gtaaatagat ggattcctat
     1921 attgtcattt cagaaaaaca gtagggacaa cttcgtaaat gatagccgta ttattaaaca
     1981 aataaattta aattagaaaa aaggaaaaaa acgcaccact tttctttttc gctgatgcac
     2041 agcttgtcgg tttgcgtgca aatcctctgt ttcacaattt tttcttcttc tctttctctc
     2101 tcttcctctt ttattcctct gttccaaagt tcagcagaag caaacacaca catcacttac
     2161 tatctctctc tccttcttca ctttctcaca taaccaaact ctctctttct ctcttttttt
     2221 tgaagtctcc tttgaaacta taattgccct ttagtgttgt tcgttcagag tcttcaaaac
     2281 ttttgcagct tcaattgtac ctgggtttct tcttcattgt tcctaaggtt tctgtgtcct
     2341 tcaattcttc tgatataatg cttctttaag agagttgaca tcatcacttt cttggggtac
     2401 tcttctctgt ttctccccag aaaatccaac tctgtaattt tgggtcttta ttctgttttt
     2461 ctctttgaag aatctttaaa attctcagat cttctgaatc tctcttcttt aaaacttttt
     2521 ttaactttat tttttgtact cgcttctttg ccttcatttt tctcgtatcc acatgtcgtt
     2581 ggtctttcgc tacaagccac gaccgtagaa tcttcttttg tctgaaaaga attacaattt
     2641 acgtttctct tacgatacga cggactttcc gaagaaatta atttaaagag aaaagaagaa
     2701 gaagccaaag aagaagaaga agctagaaga aacagtaaag tttgagactt tttttgaggg
     2761 tcgagctaaa aacaccatgg ctagtgaagc tcgaaaaaca aagaaaaaaa tcaaagggat
     2821 tcagcaagcc actgcaggag tctcacaaga cacttcggaa aatcctaaca aaacaatagt
     2881 tcctgcagca ttaccacagc tcacccctac cttggtgtca ctgctggagg tgattgaacc
     2941 cgaggtgttg tatgcaggat atgatagctc tgttccagat tcagcatgga gaattatgac
     3001 cacactcaac atgttaggtg ggcgtcaagt gattgcagca gtgaaatggg caaaggcgat
     3061 accaggcttc agaaacttac acctggatga ccaaatgacc ctgctacagt actcatggat
     3121 gtttctcatg gcatttgccc tgggttggag atcatacaga caatcaagtg gaaacctgct
     3181 ctgctttgct cctgatctga ttattaatga gcagagaatg tctctaccct gcatgtatga
     3241 ccaatgtaaa cacatgctgt ttgtctcctc tgaattacaa agattgcagg tatcctatga
     3301 agagtatctc tgtatgaaaa ccttactgct tctctcctca gttcctaagg aaggtctgaa
     3361 gagccaagag ttatttgatg agattcgaat gacttatatc aaagagctag gaaaagccat
     3421 cgtcaaaagg gaagggaact ccagtcagaa ctggcaacgg ttttaccaac tgacaaagct
     3481 tctggactcc atgcatgagg tggttgagaa tctccttacc tactgcttcc agacattttt
     3541 ggataagacc atgagtattg aattcccaga gatgttagct gaaatcatca ctaatcagat
     3601 accaaaatat tcaaatggaa atatcaaaaa gcttctgttt catcaaaaat ctactagcaa
     3661 accggtaacg ttatacgacg tcgctgaata cgccggcgtt tctcatcaaa ccgtttctag
     3721 agtggttaac caggcttcac atgttagcgc taaaacccgg gaaaaagttg aagctgccat
     3781 ggctgagctc aactacatcc cgaaccgtgt tgcgcagcag ctggctggta aacaaagctt
     3841 gctgatcggt gtcgcgacct cgagcttggc cctgcacgcg ccgtcgcaaa ttgtcgcggc
     3901 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag
     3961 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct
     4021 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa
     4081 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc
     4141 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat
     4201 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca
     4261 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc
     4321 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat
     4381 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct
     4441 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta
     4501 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
     4561 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
     4621 actggtgaaa agaaaaacca ctagtggatc ggaattcgcc aattttaatc aaagtgggaa
     4681 tattgctgat agctcattgt ccttcacttt cactaacagt agcaacggtc cgaacctcat
     4741 aacaactcaa acaaattctc aagcgctttc acaaccaatt gcctcctcta acgttcatga
     4801 taacttcatg aataatgaaa tcacggctag taaaattgat gatggtaata attcaaaacc
     4861 actgtcacct ggttggacgg accaaactgc gtataacgcg tttggaatca ctacagggat
     4921 gtttaatacc actacaatgg atgatgtata taactatcta ttcgatgatg aagatacccc
     4981 accaaaccca aaaaaagagt aactgcaaag ctttcgttcg tatcatcggt ttcgacaacg
     5041 ttcgtcaagt tcaatgcatc agtttcattg cgcacacacc agaatcctac tgagtttgag
     5101 tattatggca ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt
     5161 gttttttatt cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat
     5221 gaatgatatg gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg
     5281 ttgtgtgttg aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt
     5341 gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga gtaaaacact tgtagttgta
     5401 ccattatgct tattcactag gcaacaaata tattttcaga cctagaaaag ctgcaaatgt
     5461 tactgaatac aagtatgtcc tcttgtgttt tagacattta tgaactttcc tttatgtaat
     5521 tttccagaat ccttgtcaga ttctaatcat tgctttataa ttatagttat actcatggat
     5581 ttgtagttga gtatgaaaat attttttaat gcattttatg acttgccaat tgattgacaa
     5641 catgcatcaa tcgactatga gtaggcaaga tgttctgtag gggctatgat gcctggtcac
     5701 aacctatgca tatgtcgagc tcaagaaatc tagaaagaag aaagggaaga gaaagaattg
     5761 tgagcgctca caattgaaag actagaaaga agaaagggaa gagaaagaat tgtgagcgct
     5821 cacaattgaa agactagaaa gaagaaaggg aagagaaaga attgtgagcg ctcacaattg
     5881 aaagactaga aagaagaaag ggaagagaaa gaattgtgag cgctcacaat tgaaagacta
     5941 gtggatcgat cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
     6001 acgaacaatg aaagccttca cactcgctct cttcttagct ctttccctct atctcctgcc
     6061 caatccagcc actagtggct caacaatggt gagcaagggc gaggagctgt tcaccggggt
     6121 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     6181 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     6241 caagctgccc gtgccctggc ccaccctcgt gaccaccctg tcctggggcg tgcagtgctt
     6301 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     6361 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     6421 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     6481 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tactttagcg acaacgtcta
     6541 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     6601 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     6661 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
     6721 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     6781 cggcatggac gagctgtaca agtcaggtca tgatgagctt tagctgcatc tcgtctctgt
     6841 tatgcttaaa aagttcaatg tttcgtttca tgtaaaactt tggtggtttg tgttttgggg
     6901 ccttgtataa tccctgatga ataagtgttc tactatgttt ccgttcctgt tatctctttc
     6961 tttctaatga caagtcgaac ttcttcttta tcatcgcttc gtttttatta tctgtgcttc
     7021 ttttgtttaa tacgcctgca aagtgactcg actctgttta gtgcagttct gcgaaacttg
     7081 taaatagtcc aattgttggc ctctagtaat agatgtagcg aaagtgttga gctgttgggt
     7141 tctaaggatg gcttgaacat gttaatcttt taggttctga gtatgatgaa cattcgttgt
     7201 tgctaagaaa tgcctgtaat gtcccacaaa tgtagaaaat ggttcgtacc tttgtccaag
     7261 cattgatatg tctgatgaga ggaaactgca agatactgag cttggtttaa cgaaggagag
     7321 gcagtttctt ccttccaaag catttcattt gacaatgcct tgatcatctt aagtagagtt
     7381 tctgttgtgg aaagtttgaa actttgaaga aacgactctc aagtaaattg atgatcacaa
     7441 gtgaaagtgt atgttactag gatccttttc aaatcagtgc gcaagacgtg acgtaagtat
     7501 ccgagtcagt ttttattttt ctactaattt ggtcgtttat ttcggcgtgt aggacatggc
     7561 aaccgggcct gaatttcgcg ggtattctgt ttctattcca actttttctt gatccgcagc
     7621 cattaacgac ttttgaatag atacgctgac acgccaagcc tcgctagtca aaagtgtacc
     7681 aaacaacgct ttacagcaag aacggaatgc gcgtgacgct cgcggtgacg ccatttcgcc
     7741 ttttcagaaa tggataaata gccttgcttc ctattatatc ttcccaaatt accaatacat
     7801 tacactagca tctgaatttc ataaccaatc tcgatacacc aaatcgccat ggcttctatg
     7861 atatcctctt cagctgtgac tacagtcagc cgtgcttcta cggtgcaatc ggccgcggtg
     7921 gctccattcg gcggcctcaa atccatgact ggattcccag ttaagaaggt caacactgac
     7981 attacttcca ttacaagcaa tggtggaaga gtaaagtgca tggtgacggt gttcggcatt
     8041 ctgaatctca ccgaggactc cttcttcgat gagagccggc ggctagaccc cgccggcgct
     8101 gtcaccgcgg cgatcgaaat gctgcgagtc ggatcagacg tcgtggatgt cggaccggcc
     8161 gccagccatc cggacgcgag gcctgtatcg ccggccgatg agatcagacg tattgcgccg
     8221 ctcttagacg ccctgtccga tcagatgcac cgtgtttcaa tcgacagctt ccaaccggaa
     8281 acccagcgct atgcgctcaa gcgcggcgtg ggctacctga acgatatcca aggatttcct
     8341 gaccctgcgc tctatcccga tattgctgag gcggactgca ggctggtggt tatgcactca
     8401 gcgcagcggg atggcatcgc cacccgcacc ggtcaccttc gacccgaaga cgcgctcgac
     8461 gagattgtgc ggttcttcga ggcgcgggtt tccgccttgc gacggagcgg ggtcgctgcc
     8521 gaccggctca tcctcgatcc ggggatggga tttttcttga gccccgcacc ggaaacatcg
     8581 ctgcacgtgc tgtcgaacct tcaaaagctg aagtcggcgt tggggcttcc gctacttgtc
     8641 tcggtgtcgc ggaaatcctt cttgggcgcc accgttggcc ttcctgtaaa ggatctgggt
     8701 ccagcgagcc ttgcggcgga acttcacgcg atcggcaatg gcgctgacta cgtccgcacc
     8761 cacgcgcctg gagatctgcg aagcgcaatc accatctcgg aaaccctcgc gaaatttcgc
     8821 agtcgcgacg ccagagacag agggttagat catgcctagc ttggctgcag gtcgacctgc
     8881 aggcatgccc gctgaaatca ccagtctctc tctacaaatc tatctctctc tataataatg
     8941 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag
     9001 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     9061 taattcctaa aaccaaaatc caggggtacc gtattctaga ccaagcatgc acagctggcg
     9121 ttctcagcca gatccccttg acaggatata ttggcgggta aactaagtcg ctgtatgtgt
     9181 ttgtttgaga tctcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9241 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9721 tgaagccagt taccttcgga agaagagttg gtagctcttg atccggcaaa caaaccaccg
     9781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9961 aatgaagttt taaatcaatc taaagtatat atgtgtaaca ttggtctagt gattatttgc
    10021 cgactacctt ggtgatctcg cctttcacgt agtgaacaaa ttcttccaac tgatctgcgc
    10081 gcgaggccaa gcgatcttct tgtccaagat aagcctgcct agcttcaagt atgacgggct
    10141 gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt
    10201 tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc
    10261 cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat
    10321 cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat
    10381 gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga
    10441 tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat
    10501 aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct
    10561 cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg
    10621 tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc
    10681 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct
    10741 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca
    10801 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat
    10861 ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa
    10921 atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt
    10981 ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca
    11041 accgtggctc cctcactttc tggctggatg atggggcgat tcaggcgatc cccatccaac
    11101 agcccgccgt cgagcgggct
//
