LOCUS       sequence-220088-        9389 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral backbone for Gateway cloning of Promoter/Gene insert 
            together with constitutive Puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9389)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 9389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWX-R4DESTR2-PGK-PURO
FEATURES             Location/Qualifiers
     source          1..9389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(147..734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(227..246)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(905..1696,1697..1765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1528..1547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1766..1870)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1901..2280
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             2282..2539
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2586..2711
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3203..3320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3680..3913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4098..4142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    4417..4540
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4565..4570,4571..4588,4589..4595)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4649..5308
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4715..4734)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5650..5955
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5834..5853
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6010..6134)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6151..6661
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6580..6598
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6682..7281
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6682..6701)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7178..7198
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7301..7889
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7354..7374)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7772..7783)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7892..7908)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7893..7909)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(8037..8070)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8090..8270
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8308..8327)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8373..8428
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8427..8446)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8789..8805)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8789..8805)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8802..8824)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8813..8829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8837..8843,8844..8861,8862..8867))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8882..8903
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8962..9157
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9008..9143
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9163..9297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9200..9219)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9254..9273
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9365..9382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
       61 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      121 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      181 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      241 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct tacggatacc
      301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1021 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1861 gggttccgcg cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt
     1921 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     1981 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     2041 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     2101 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2161 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2221 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2281 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     2341 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     2401 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     2461 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     2521 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     2581 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     2641 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     2701 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     2761 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     2821 aacgattcgc agttaatact ggcctgttag aaacatcaga aggctgtaga caaatactgg
     2881 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     2941 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     3001 acaagataga ggaagagcaa aacaaaagta agaaaaaagc acagcaagca gcaggatctt
     3061 cagacctgga aattccctac aatccccaaa gtcaaggagt agtagaatct atgaataaag
     3121 aattaaagaa aattatagga caggtaagag atcaggctga acatcttaag acagcagtac
     3181 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     3241 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     3301 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagaaatcca ctttggaaag
     3361 gaccagcaaa gctcctctgg aaaggtgaag gggcagtagt aatacaagat aatagtgaca
     3421 taaaagtagt gccaagaaga aaagcaaaga tcattaggga ttatggaaaa cagatggcag
     3481 gtgatgattg tgtggcaagt agacaggatg aggattagaa catggaaaag tttagtaaaa
     3541 caccataagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     3601 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     3661 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     3721 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     3781 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     3841 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     3901 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     3961 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     4021 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     4081 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     4141 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     4201 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     4261 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     4321 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     4381 tgaacggatc tcgacggtat ctagtgatta tcggatcaac tttgtataga aaagttgaac
     4441 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
     4501 ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc
     4561 aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc
     4621 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
     4681 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
     4741 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
     4801 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
     4861 ggaactccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
     4921 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
     4981 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
     5041 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
     5101 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
     5161 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
     5221 tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
     5281 ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa aagccagata
     5341 acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac
     5401 ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc
     5461 gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa
     5521 ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag
     5581 ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg
     5641 gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt
     5701 gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc
     5761 agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg
     5821 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg
     5881 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
     5941 ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg tcgagcagat
     6001 ctgctcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     6061 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     6121 ttgtacaaag tggtgataat cgaattccac ggggttgggg ttgcgccttt tccaaggcag
     6181 ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc
     6241 cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc
     6301 tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt
     6361 gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc
     6421 agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag
     6481 cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg
     6541 gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc
     6601 tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca
     6661 gggggatcca ccggtgccac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     6721 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6781 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6841 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6901 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6961 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     7021 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     7081 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     7141 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7201 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7261 acccgcaagc ccggtgcctg agataatcaa gcttatcgat aatcaacctc tggattacaa
     7321 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7381 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7441 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7501 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7561 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7621 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7681 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7741 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7801 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7861 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag gttcgcttta
     7921 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7981 ctggaagggc taattcactc ccaacgaaga caagatggga tcaattcacc atgggaataa
     8041 cttcgtatag catacattat acgaagttat gctgcttttt gcttgtactg ggtctctctg
     8101 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8161 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8221 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagtt
     8281 gaatcgccag tgtgtcgagc tagcagatct ttttccctct gccaaaaatt atggggacat
     8341 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     8401 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     8461 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     8521 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     8581 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     8641 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     8701 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     8761 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8821 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8881 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8941 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
     9001 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9061 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9121 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct
     9181 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9241 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc
     9301 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     9361 cttcctcgct cactgactcg ctgcgctcg
//
