LOCUS       sequence-220479-       15786 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15786)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      13839..13848
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14534..14557
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             14579..14608
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14609..14632
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14722..14742)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15140..15151)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15376..15510
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15413..15432)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15467..15486
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15539..15704
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggcga ccctggaaaa
     5101 gctgatgaag gccttcgagt ccctcaagtc cttccaacag cagcaacagc aacaacagca
     5161 gcaacagcaa caacagcagc aacagcaaca acagcagcaa cagcaacaac cgccaccgcc
     5221 gccgccgccg ccgccgcctc ctcagcttcc tcagccgccg ccgcaggcac agccgctgct
     5281 gcctcagccg cagccgcccc cgccgccgcc cccgccgcca cccggcccgg ctgtggctga
     5341 ggagccgctg caccgaccaa agaaagaact ttcagctacc aagaaagacc gtgtgaatca
     5401 ttgtctgaca atatgtgaaa acatagtggc acagtctgtc agaaattctc cagaatttca
     5461 gaaacttctg ggcatcgcta tggaactttt tctgctgtgc agtgatgacg cagagtcaga
     5521 tgtcaggatg gtggctgacg aatgcctcaa caaagttatc aaagctttga tggattctaa
     5581 tcttccaagg ttacagctcg agctctataa ggaaattaaa aagaatggtg cccctcggag
     5641 tttgcgtgct gccctgtgga ggtttgctga gctggctcac ctggttcggc ctcagaaatg
     5701 caggccttac ctggtgaacc ttctgccgtg cctgactcga acaagcaaga gacccgaaga
     5761 atcagtccag gagaccttgg ctgcagctgt tcccaaaatt atggcttctt ttggcaattt
     5821 tgcaaatgac aatgaaatta aggttttgtt aaaggccttc atagcgaacc tgaagtcaag
     5881 ctcccccacc attcggcgga cagcggctgg atcagcagtg agcatctgcc agcactcaag
     5941 aaggacacaa tatttctata gttggctact aaatgtgctc ttaggcttac tcgttcctgt
     6001 cgaggatgaa cactccactc tgctgattct tggcgtgctg ctcaccctga ggtatttggt
     6061 gcccttgctg cagcagcagg tcaaggacac aagcctgaaa ggcagcttcg gagtgacaag
     6121 gaaagaaatg gaagtctctc cttctgcaga gcagcttgtc caggtttatg aactgacgtt
     6181 acatcataca cagcaccaag accacaatgt tgtgaccgga gccctggagc tgttgcagca
     6241 gctcttcaga acgcctccac ccgagcttct gcaaaccctg accgcagtcg ggggcattgg
     6301 gcagctcacc gctgctaagg aggagtctgg tggccgaagc cgtagtggga gtattgtgga
     6361 acttatagct ggagggggtt cctcatgcag ccctgtcctt tcaagaaaac aaaaaggcaa
     6421 agtgctctta ggagaagaag aagccttgga ggatgactct gaatcgagat cggatgtcag
     6481 cagctctgcc ttaacagcct cagtgaagga tgagatcagt ggagagctgg ctgcttcttc
     6541 aggggtttcc actccagggt cagcaggtca tgacatcatc acagaacagc cacggtcaca
     6601 gcacacactg caggcggact cagtggatct ggccagctgt gacttgacaa gctctgccac
     6661 tgatggggat gaggaggata tcttgagcca cagctccagc caggtcagcg ccgtcccatc
     6721 tgaccctgcc atggacctga atgatgggac ccaggcctcg tcgcccatca gcgacagctc
     6781 ccagaccacc accgaagggc ctgattcagc tgttacccct tcagacagtt ctgaaattgt
     6841 gttagacggt accgacaacc agtatttggg cctgcagatt ggacagcccc aggatgaaga
     6901 tgaggaagcc acaggtattc ttcctgatga agcctcggag gccttcagga actcttccat
     6961 ggcccttcaa caggcacatt tattgaaaaa catgagtcac tgcaggcagc cttctgacag
     7021 cagtgttgat aaatttgtgt tgagagatga agctactgaa ccgggtgatc aagaaaacaa
     7081 gccttgccgc atcaaaggtg acattggaca gtccactgat gatgactctg cacctcttgt
     7141 ccattgtgtc cgccttttat ctgcttcgtt tttgctaaca gggggaaaaa atgtgctggt
     7201 tccggacagg gatgtgaggg tcagcgtgaa ggccctggcc ctcagctgtg tgggagcagc
     7261 tgtggccctc cacccggaat ctttcttcag caaactctat aaagttcctc ttgacaccac
     7321 ggaataccct gaggaacagt atgtctcaga catcttgaac tacatcgatc atggagaccc
     7381 acaggttcga ggagccactg ccattctctg tgggaccctc atctgctcca tcctcagcag
     7441 gtcccgcttc cacgtgggag attggatggg caccattaga accctcacag gaaatacatt
     7501 ttctttggcg gattgcattc ctttgctgcg gaaaacactg aaggatgagt cttctgttac
     7561 ttgcaagtta gcttgtacag ctgtgaggaa ctgtgtcatg agtctctgca gcagcagcta
     7621 cagtgagtta ggactgcagc tgatcatcga tgtgctgact ctgaggaaca gttcctattg
     7681 gctggtgagg acagagcttc tggaaaccct tgcagagatt gacttcaggc tggtgagctt
     7741 tttggaggca aaagcagaaa acttacacag aggggctcat cattatacag ggcttttaaa
     7801 actgcaagaa cgagtgctca ataatgttgt catccatttg cttggagatg aagaccccag
     7861 ggtgcgacat gttgccgcag catcactaat taggcttgtc ccaaagctgt tttataaatg
     7921 tgaccaagga caagctgatc cagtagtggc cgtggcaaga gatcaaagca gtgtttacct
     7981 gaaacttctc atgcatgaga cgcagcctcc atctcatttc tccgtcagca caataaccag
     8041 aatatataga ggctataacc tactaccaag cataacagac gtcactatgg aaaataacct
     8101 ttcaagagtt attgcagcag tttctcatga actaatcaca tcaaccacca gagcactcac
     8161 atttggatgc tgtgaagctt tgtgtcttct ttccactgcc ttcccagttt gcatttggag
     8221 tttaggttgg cactgtggag tgcctccact gagtgcctca gatgagtcta ggaagagctg
     8281 taccgttggg atggccacaa tgattctgac cctgctctcg tcagcttggt tcccattgga
     8341 tctctcagcc catcaagatg ctttgatttt ggccggaaac ttgcttgcag ccagtgctcc
     8401 caaatctctg agaagttcat gggcctctga agaagaagcc aacccagcag ccaccaagca
     8461 agaggaggtc tggccagccc tgggggaccg ggccctggtg cccatggtgg agcagctctt
     8521 ctctcacctg ctgaaggtga ttaacatttg tgcccacgtc ctggatgacg tggctcctgg
     8581 acccgcaata aaggcagcct tgccttctct aacaaacccc ccttctctaa gtcccatccg
     8641 acgaaagggg aaggagaaag aaccaggaga acaagcatct gtaccgttga gtcccaagaa
     8701 aggcagtgag gccagtgcag cttctagaca atctgatacc tcaggtcctg ttacaacaag
     8761 taaatcctca tcactgggga gtttctatca tcttccttca tacctcaaac tgcatgatgt
     8821 cctgaaagct acacacgcta actacaaggt cacgctggat cttcagaaca gcacggaaaa
     8881 gtttggaggg tttcttcgct cagccttgga tgttctttct cagatactag agctggccac
     8941 actgcaggac attgggaagt gtgttgaaga gatcctagga tacctgaaat cctgctttag
     9001 tcgagaacca atgatggcaa ctgtttgtgt tcaacaattg ttgaagactc tctttggcac
     9061 aaacttggcc tcccagtttg atggcttatc ttccaacccc agcaagtcac aaggccgagc
     9121 acagcgcctt ggctcctcca gtgtgaggcc aggcttgtac cactactgct tcatggcccc
     9181 gtacacccac ttcacccagg ccctcgctga cgccagcctg aggaacatgg tgcaggcgga
     9241 gcaggagaac gacacctcgg gatggtttga tgtcctccag aaagtgtcta cccagttgaa
     9301 gacaaacctc acgagtgtca caaagaaccg tgcagataag aatgctattc ataatcacat
     9361 tcgtttgttt gaacctcttg ttataaaagc tttaaaacag tacacgacta caacatgtgt
     9421 gcagttacag aagcaggttt tagatttgct ggcgcagctg gttcagttac gggttaatta
     9481 ctgtcttctg gattcagatc aggtgtttat tggctttgta ttgaaacagt ttgaatacat
     9541 tgaagtgggc cagttcaggg aatcagaggc aatcattcca aacatctttt tcttcttggt
     9601 attactatct tatgaacgct atcattcaaa acagatcatt ggaattccta aaatcattca
     9661 gctctgtgat ggcatcatgg ccagtggaag gaaggctgtg acacatgcca taccggctct
     9721 gcagcccata gtccacgacc tctttgtatt aagaggaaca aataaagctg atgcaggaaa
     9781 agagcttgaa acccaaaaag aggtggtggt gtcaatgtta ctgagactca tccagtacca
     9841 tcaggtgttg gagatgttca ttcttgtcct gcagcagtgc cacaaggaga atgaagacaa
     9901 gtggaagcga ctgtctcgac agatagctga catcatcctc ccaatgttag ccaaacagca
     9961 gatgcacatt gactctcatg aagcccttgg agtgttaaat acattatttg agattttggc
    10021 cccttcctcc ctccgtccgg tagacatgct tttacggagt atgttcgtca ctccaaacac
    10081 aatggcgtcc gtgagcactg ttcaactgtg gatatcggga attctggcca ttttgagggt
    10141 tctgatttcc cagtcaactg aagatattgt tctttctcgt attcaggagc tctccttctc
    10201 tccgtattta atctcctgta cagtaattaa taggttaaga gatggggaca gtacttcaac
    10261 gctagaagaa cacagtgaag ggaaacaaat aaagaatttg ccagaagaaa cattttcaag
    10321 gtttctatta caactggttg gtattctttt agaagacatt gttacaaaac agctgaaggt
    10381 ggaaatgagt gagcagcaac atactttcta ttgccaggaa ctaggcacac tgctaatgtg
    10441 tctgatccac atcttcaagt ctggaatgtt ccggagaatc acagcagctg ccactaggct
    10501 gttccgcagt gatggctgtg gcggcagttt ctacaccctg gacagcttga acttgcgggc
    10561 tcgttccatg atcaccaccc acccggccct ggtgctgctc tggtgtcaga tactgctgct
    10621 tgtcaaccac accgactacc gctggtgggc agaagtgcag cagaccccga aaagacacag
    10681 tctgtccagc acaaagttac ttagtcccca gatgtctgga gaagaggagg attctgactt
    10741 ggcagccaaa cttggaatgt gcaatagaga aatagtacga agaggggctc tcattctctt
    10801 ctgtgattat gtctgtcaga acctccatga ctccgagcac ttaacgtggc tcattgtaaa
    10861 tcacattcaa gatctgatca gcctttccca cgagcctcca gtacaggact tcatcagtgc
    10921 cgttcatcgg aactctgctg ccagcggcct gttcatccag gcaattcagt ctcgttgtga
    10981 aaacctttca actccaacca tgctgaagaa aactcttcag tgcttggagg ggatccatct
    11041 cagccagtcg ggagctgtgc tcacgctgta tgtggacagg cttctgtgca cccctttccg
    11101 tgtgctggct cgcatggtcg acatccttgc ttgtcgccgg gtagaaatgc ttctggctgc
    11161 aaatttacag agcagcatgg cccagttgcc aatggaagaa ctcaacagaa tccaggaata
    11221 ccttcagagc agcgggctcg ctcagagaca ccaaaggctc tattccctgc tggacaggtt
    11281 tcgtctctcc accatgcaag actcacttag tccctctcct ccagtctctt cccacccgct
    11341 ggacggggat gggcacgtgt cactggaaac agtgagtccg gacaaagact ggtacgttca
    11401 tcttgtcaaa tcccagtgtt ggaccaggtc agattctgca ctgctggaag gtgcagagct
    11461 ggtgaatcgg attcctgctg aagatatgaa tgccttcatg atgaactcgg agttcaacct
    11521 aagcctgcta gctccatgct taagcctagg gatgagtgaa atttctggtg gccagaagag
    11581 tgcccttttt gaagcagccc gtgaggtgac tctggcccgt gtgagcggca ccgtgcagca
    11641 gctccctgct gtccatcatg tcttccagcc cgagctgcct gcagaaccgg cggcctactg
    11701 gagcaagttg aatgatctgt ttggggatgc tgcactgtat cagtccctgc ccactctggc
    11761 ccgggccctg gcacagtacc tggtggtggt ctccaaactg cccagtcatt tgcaccttcc
    11821 tcctgagaaa gagaaggaca ttgtgaaatt cgtggtggca acccttgagg ccctgtcctg
    11881 gcatttgatc catgagcaga tcccgctgag tctggatctc caggcagggc tggactgctg
    11941 ctgcctggcc ctgcagctgc ctggcctctg gagcgtggtc tcctccacag agtttgtgac
    12001 ccacgcctgc tccctcatcc actgtgtgca cttcatcctg gaggccgttg cagtgcagcc
    12061 tggagagcag cttcttagtc cagaaagaag gacaaatacc ccaaaagcca tcagcgagga
    12121 ggaggaggaa gtagatccaa acacacagaa tcctaagtat atcactgcag cctgtgagat
    12181 ggtggcagaa atggtggagt ctctgcagtc ggtgttggcc ttgggtcata aaaggaatag
    12241 cggcgtgccg gcgtttctca cgccattgct caggaacatc atcatcagcc tggcccgcct
    12301 gccccttgtc aacagctaca cacgtgtgcc cccactggtg tggaagcttg gatggtcacc
    12361 caaaccggga ggggattttg gcacagcatt ccctgagatc cccgtggagt tcctccagga
    12421 aaaggaagtc tttaaggagt tcatctaccg catcaacaca ctaggctgga ccagtcgtac
    12481 tcagtttgaa gaaacttggg ccaccctcct tggtgtcctg gtgacgcagc ccctcgtgat
    12541 ggagcaggag gagagcccac cagaagaaga cacagagagg acccagatca acgtcctggc
    12601 cgtgcaggcc atcacctcac tggtgctcag tgcaatgact gtgcctgtgg ccggcaaccc
    12661 agctgtaagc tgcttggagc agcagccccg gaacaagcct ctgaaagctc tcgacaccag
    12721 gtttgggagg aagctgagca ttatcagagg gattgtggag caagagattc aagcaatggt
    12781 ttcaaagaga gagaatattg ccacccatca tttatatcag gcatgggatc ctgtcccttc
    12841 tctgtctccg gctactacag gtgccctcat cagccacgag aagctgctgc tgcagatcaa
    12901 ccccgagcgg gagctgggga gcatgagcta caaactcggc caggtgtcca tacactccgt
    12961 gtggctaggg aacagcatca cacccctgag ggaggaggaa tgggacgagg aagaggagga
    13021 ggaggccgac gcccctgcac cttcgtcacc acccacgtct ccagtcaact ccaggaaaca
    13081 ccgggctgga gttgacatcc actcctgttc gcagtttttg cttgagttgt acagccgctg
    13141 gatcctgccg tccagctcag ccaggaggac cccggccatc ctgatcagtg aggtggtcag
    13201 atcccttcta gtggtctcag acttgttcac cgagcgcaac cagtttgagc tgatgtatgt
    13261 gacgctgaca gaactgcgaa gggtgcaccc ttcagaagac gagatcctcg ctcagtacct
    13321 ggtgcctgcc acctgcaagg cagctgccgt ccttgggatg gacaaggccg tggcggagcc
    13381 tgtcagccgc ctgctggaga gcacgctcag gagcagccac ctgcccagca gggttggagc
    13441 cctgcacggc atcctctatg tgctggagtg cgacctgctg gacgacactg ccaagcagct
    13501 catcccggtc atcagcgact atctcctctc caacctgaaa gggatcgccc actgcgtgaa
    13561 cattcacagc cagcagcacg tactggtcat gtgtgccact gcgttttacc tcattgagaa
    13621 ctatcctctg gacgtagggc cggaattttc agcatcaata atacagatgt gtggggtgat
    13681 gctgtctgga agtgaggagt ccaccccctc catcatttac cactgtgccc tcagaggcct
    13741 ggagcgcctc ctgctctctg agcagctctc ccgcctggat gcagaatcgc tggtcaagct
    13801 gagtgtggac agagtgaacg tgcacagccc gcaccgggcc atggcggctc tgggcctgat
    13861 gctcacctgc atgtacacag gaaaggagaa agtcagtccg ggtagaactt cagaccctaa
    13921 tcctgcagcc cccgacagcg agtcagtgat tgttgctatg gagcgggtat ctgttctttt
    13981 tgataggatc aggaaaggct ttccttgtga agccagagtg gtggccagga tcctgcccca
    14041 gtttctagac gacttcttcc caccccagga catcatgaac aaagtcatcg gagagtttct
    14101 gtccaaccag cagccatacc cccagttcat ggccaccgtg gtgtataagg tgtttcagac
    14161 tctgcacagc accgggcagt cgtccatggt ccgggactgg gtcatgctgt ccctctccaa
    14221 cttcacgcag agggccccgg tcgccatggc cacgtggagc ctctcctgct tctttgtcag
    14281 cgcgtccacc agcccgtggg tcgcggcgat cctcccacat gtcatcagca ggatgggcaa
    14341 gctggagcag gtggacgtga accttttctg cctggtcgcc acagacttct acagacacca
    14401 gatagaggag gagctcgacc gcagggcctt ccagtctgtg cttgaggtgg ttgcagcccc
    14461 aggaagccca tatcaccggc tgctgacttg tttacgaaat gtccacaagg tcaccacctg
    14521 cggtggaagc ggtgactaca aagacgatga cgacaagtaa aacctctact tccaatcgca
    14581 ccatcatcac catcaccatc accaccatga ttacaaggat gacgacgata agtgaggcgg
    14641 ccgccaactt gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
    14701 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
    14761 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
    14821 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
    14881 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
    14941 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
    15001 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
    15061 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
    15121 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcgggcgccc
    15181 ccctcctcac ggcgagcgaa gcttgtcgag aagtactaga ggatctctag agcctgcagt
    15241 ctcgacaagc ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag
    15301 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    15361 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    15421 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    15481 ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag gagatccgaa
    15541 ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta
    15601 cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact
    15661 ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt
    15721 cctgttatgt ttttaatcaa acatcctgcc aactccatgt gacaaaccgt catcttcggc
    15781 tacttt
//
