LOCUS       sequence-220484-       15837 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15837)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      13890..13899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14585..14608
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             14630..14659
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14660..14683
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14773..14793)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15191..15202)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15427..15561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15464..15483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15518..15537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15590..15755
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggcga ccctggaaaa
     5101 gctgatgaag gccttcgagt ccctcaagtc cttccaacag cagcaacagc aacaacagca
     5161 gcaacagcaa caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca
     5221 gcaacaacag cagcaacagc aacaacagca gcaacagcaa ccgccaccgc cgccgccgcc
     5281 gccgccgcct cctcagcttc ctcagccgcc gccgcaggca cagccgctgc tgcctcagcc
     5341 gcagccgccc ccgccgccgc ccccgccgcc acccggcccg gctgtggctg aggagccgct
     5401 gcaccgacca aagaaagaac tttcagctac caagaaagac cgtgtgaatc attgtctgac
     5461 aatatgtgaa aacatagtgg cacagtctgt cagaaattct ccagaatttc agaaacttct
     5521 gggcatcgct atggaacttt ttctgctgtg cagtgatgac gcagagtcag atgtcaggat
     5581 ggtggctgac gaatgcctca acaaagttat caaagctttg atggattcta atcttccaag
     5641 gttacagctc gagctctata aggaaattaa aaagaatggt gcccctcgga gtttgcgtgc
     5701 tgccctgtgg aggtttgctg agctggctca cctggttcgg cctcagaaat gcaggcctta
     5761 cctggtgaac cttctgccgt gcctgactcg aacaagcaag agacccgaag aatcagtcca
     5821 ggagaccttg gctgcagctg ttcccaaaat tatggcttct tttggcaatt ttgcaaatga
     5881 caatgaaatt aaggttttgt taaaggcctt catagcgaac ctgaagtcaa gctcccccac
     5941 cattcggcgg acagcggctg gatcagcagt gagcatctgc cagcactcaa gaaggacaca
     6001 atatttctat agttggctac taaatgtgct cttaggctta ctcgttcctg tcgaggatga
     6061 acactccact ctgctgattc ttggcgtgct gctcaccctg aggtatttgg tgcccttgct
     6121 gcagcagcag gtcaaggaca caagcctgaa aggcagcttc ggagtgacaa ggaaagaaat
     6181 ggaagtctct ccttctgcag agcagcttgt ccaggtttat gaactgacgt tacatcatac
     6241 acagcaccaa gaccacaatg ttgtgaccgg agccctggag ctgttgcagc agctcttcag
     6301 aacgcctcca cccgagcttc tgcaaaccct gaccgcagtc gggggcattg ggcagctcac
     6361 cgctgctaag gaggagtctg gtggccgaag ccgtagtggg agtattgtgg aacttatagc
     6421 tggagggggt tcctcatgca gccctgtcct ttcaagaaaa caaaaaggca aagtgctctt
     6481 aggagaagaa gaagccttgg aggatgactc tgaatcgaga tcggatgtca gcagctctgc
     6541 cttaacagcc tcagtgaagg atgagatcag tggagagctg gctgcttctt caggggtttc
     6601 cactccaggg tcagcaggtc atgacatcat cacagaacag ccacggtcac agcacacact
     6661 gcaggcggac tcagtggatc tggccagctg tgacttgaca agctctgcca ctgatgggga
     6721 tgaggaggat atcttgagcc acagctccag ccaggtcagc gccgtcccat ctgaccctgc
     6781 catggacctg aatgatggga cccaggcctc gtcgcccatc agcgacagct cccagaccac
     6841 caccgaaggg cctgattcag ctgttacccc ttcagacagt tctgaaattg tgttagacgg
     6901 taccgacaac cagtatttgg gcctgcagat tggacagccc caggatgaag atgaggaagc
     6961 cacaggtatt cttcctgatg aagcctcgga ggccttcagg aactcttcca tggcccttca
     7021 acaggcacat ttattgaaaa acatgagtca ctgcaggcag ccttctgaca gcagtgttga
     7081 taaatttgtg ttgagagatg aagctactga accgggtgat caagaaaaca agccttgccg
     7141 catcaaaggt gacattggac agtccactga tgatgactct gcacctcttg tccattgtgt
     7201 ccgcctttta tctgcttcgt ttttgctaac agggggaaaa aatgtgctgg ttccggacag
     7261 ggatgtgagg gtcagcgtga aggccctggc cctcagctgt gtgggagcag ctgtggccct
     7321 ccacccggaa tctttcttca gcaaactcta taaagttcct cttgacacca cggaataccc
     7381 tgaggaacag tatgtctcag acatcttgaa ctacatcgat catggagacc cacaggttcg
     7441 aggagccact gccattctct gtgggaccct catctgctcc atcctcagca ggtcccgctt
     7501 ccacgtggga gattggatgg gcaccattag aaccctcaca ggaaatacat tttctttggc
     7561 ggattgcatt cctttgctgc ggaaaacact gaaggatgag tcttctgtta cttgcaagtt
     7621 agcttgtaca gctgtgagga actgtgtcat gagtctctgc agcagcagct acagtgagtt
     7681 aggactgcag ctgatcatcg atgtgctgac tctgaggaac agttcctatt ggctggtgag
     7741 gacagagctt ctggaaaccc ttgcagagat tgacttcagg ctggtgagct ttttggaggc
     7801 aaaagcagaa aacttacaca gaggggctca tcattataca gggcttttaa aactgcaaga
     7861 acgagtgctc aataatgttg tcatccattt gcttggagat gaagacccca gggtgcgaca
     7921 tgttgccgca gcatcactaa ttaggcttgt cccaaagctg ttttataaat gtgaccaagg
     7981 acaagctgat ccagtagtgg ccgtggcaag agatcaaagc agtgtttacc tgaaacttct
     8041 catgcatgag acgcagcctc catctcattt ctccgtcagc acaataacca gaatatatag
     8101 aggctataac ctactaccaa gcataacaga cgtcactatg gaaaataacc tttcaagagt
     8161 tattgcagca gtttctcatg aactaatcac atcaaccacc agagcactca catttggatg
     8221 ctgtgaagct ttgtgtcttc tttccactgc cttcccagtt tgcatttgga gtttaggttg
     8281 gcactgtgga gtgcctccac tgagtgcctc agatgagtct aggaagagct gtaccgttgg
     8341 gatggccaca atgattctga ccctgctctc gtcagcttgg ttcccattgg atctctcagc
     8401 ccatcaagat gctttgattt tggccggaaa cttgcttgca gccagtgctc ccaaatctct
     8461 gagaagttca tgggcctctg aagaagaagc caacccagca gccaccaagc aagaggaggt
     8521 ctggccagcc ctgggggacc gggccctggt gcccatggtg gagcagctct tctctcacct
     8581 gctgaaggtg attaacattt gtgcccacgt cctggatgac gtggctcctg gacccgcaat
     8641 aaaggcagcc ttgccttctc taacaaaccc cccttctcta agtcccatcc gacgaaaggg
     8701 gaaggagaaa gaaccaggag aacaagcatc tgtaccgttg agtcccaaga aaggcagtga
     8761 ggccagtgca gcttctagac aatctgatac ctcaggtcct gttacaacaa gtaaatcctc
     8821 atcactgggg agtttctatc atcttccttc atacctcaaa ctgcatgatg tcctgaaagc
     8881 tacacacgct aactacaagg tcacgctgga tcttcagaac agcacggaaa agtttggagg
     8941 gtttcttcgc tcagccttgg atgttctttc tcagatacta gagctggcca cactgcagga
     9001 cattgggaag tgtgttgaag agatcctagg atacctgaaa tcctgcttta gtcgagaacc
     9061 aatgatggca actgtttgtg ttcaacaatt gttgaagact ctctttggca caaacttggc
     9121 ctcccagttt gatggcttat cttccaaccc cagcaagtca caaggccgag cacagcgcct
     9181 tggctcctcc agtgtgaggc caggcttgta ccactactgc ttcatggccc cgtacaccca
     9241 cttcacccag gccctcgctg acgccagcct gaggaacatg gtgcaggcgg agcaggagaa
     9301 cgacacctcg ggatggtttg atgtcctcca gaaagtgtct acccagttga agacaaacct
     9361 cacgagtgtc acaaagaacc gtgcagataa gaatgctatt cataatcaca ttcgtttgtt
     9421 tgaacctctt gttataaaag ctttaaaaca gtacacgact acaacatgtg tgcagttaca
     9481 gaagcaggtt ttagatttgc tggcgcagct ggttcagtta cgggttaatt actgtcttct
     9541 ggattcagat caggtgttta ttggctttgt attgaaacag tttgaataca ttgaagtggg
     9601 ccagttcagg gaatcagagg caatcattcc aaacatcttt ttcttcttgg tattactatc
     9661 ttatgaacgc tatcattcaa aacagatcat tggaattcct aaaatcattc agctctgtga
     9721 tggcatcatg gccagtggaa ggaaggctgt gacacatgcc ataccggctc tgcagcccat
     9781 agtccacgac ctctttgtat taagaggaac aaataaagct gatgcaggaa aagagcttga
     9841 aacccaaaaa gaggtggtgg tgtcaatgtt actgagactc atccagtacc atcaggtgtt
     9901 ggagatgttc attcttgtcc tgcagcagtg ccacaaggag aatgaagaca agtggaagcg
     9961 actgtctcga cagatagctg acatcatcct cccaatgtta gccaaacagc agatgcacat
    10021 tgactctcat gaagcccttg gagtgttaaa tacattattt gagattttgg ccccttcctc
    10081 cctccgtccg gtagacatgc ttttacggag tatgttcgtc actccaaaca caatggcgtc
    10141 cgtgagcact gttcaactgt ggatatcggg aattctggcc attttgaggg ttctgatttc
    10201 ccagtcaact gaagatattg ttctttctcg tattcaggag ctctccttct ctccgtattt
    10261 aatctcctgt acagtaatta ataggttaag agatggggac agtacttcaa cgctagaaga
    10321 acacagtgaa gggaaacaaa taaagaattt gccagaagaa acattttcaa ggtttctatt
    10381 acaactggtt ggtattcttt tagaagacat tgttacaaaa cagctgaagg tggaaatgag
    10441 tgagcagcaa catactttct attgccagga actaggcaca ctgctaatgt gtctgatcca
    10501 catcttcaag tctggaatgt tccggagaat cacagcagct gccactaggc tgttccgcag
    10561 tgatggctgt ggcggcagtt tctacaccct ggacagcttg aacttgcggg ctcgttccat
    10621 gatcaccacc cacccggccc tggtgctgct ctggtgtcag atactgctgc ttgtcaacca
    10681 caccgactac cgctggtggg cagaagtgca gcagaccccg aaaagacaca gtctgtccag
    10741 cacaaagtta cttagtcccc agatgtctgg agaagaggag gattctgact tggcagccaa
    10801 acttggaatg tgcaatagag aaatagtacg aagaggggct ctcattctct tctgtgatta
    10861 tgtctgtcag aacctccatg actccgagca cttaacgtgg ctcattgtaa atcacattca
    10921 agatctgatc agcctttccc acgagcctcc agtacaggac ttcatcagtg ccgttcatcg
    10981 gaactctgct gccagcggcc tgttcatcca ggcaattcag tctcgttgtg aaaacctttc
    11041 aactccaacc atgctgaaga aaactcttca gtgcttggag gggatccatc tcagccagtc
    11101 gggagctgtg ctcacgctgt atgtggacag gcttctgtgc acccctttcc gtgtgctggc
    11161 tcgcatggtc gacatccttg cttgtcgccg ggtagaaatg cttctggctg caaatttaca
    11221 gagcagcatg gcccagttgc caatggaaga actcaacaga atccaggaat accttcagag
    11281 cagcgggctc gctcagagac accaaaggct ctattccctg ctggacaggt ttcgtctctc
    11341 caccatgcaa gactcactta gtccctctcc tccagtctct tcccacccgc tggacgggga
    11401 tgggcacgtg tcactggaaa cagtgagtcc ggacaaagac tggtacgttc atcttgtcaa
    11461 atcccagtgt tggaccaggt cagattctgc actgctggaa ggtgcagagc tggtgaatcg
    11521 gattcctgct gaagatatga atgccttcat gatgaactcg gagttcaacc taagcctgct
    11581 agctccatgc ttaagcctag ggatgagtga aatttctggt ggccagaaga gtgccctttt
    11641 tgaagcagcc cgtgaggtga ctctggcccg tgtgagcggc accgtgcagc agctccctgc
    11701 tgtccatcat gtcttccagc ccgagctgcc tgcagaaccg gcggcctact ggagcaagtt
    11761 gaatgatctg tttggggatg ctgcactgta tcagtccctg cccactctgg cccgggccct
    11821 ggcacagtac ctggtggtgg tctccaaact gcccagtcat ttgcaccttc ctcctgagaa
    11881 agagaaggac attgtgaaat tcgtggtggc aacccttgag gccctgtcct ggcatttgat
    11941 ccatgagcag atcccgctga gtctggatct ccaggcaggg ctggactgct gctgcctggc
    12001 cctgcagctg cctggcctct ggagcgtggt ctcctccaca gagtttgtga cccacgcctg
    12061 ctccctcatc cactgtgtgc acttcatcct ggaggccgtt gcagtgcagc ctggagagca
    12121 gcttcttagt ccagaaagaa ggacaaatac cccaaaagcc atcagcgagg aggaggagga
    12181 agtagatcca aacacacaga atcctaagta tatcactgca gcctgtgaga tggtggcaga
    12241 aatggtggag tctctgcagt cggtgttggc cttgggtcat aaaaggaata gcggcgtgcc
    12301 ggcgtttctc acgccattgc tcaggaacat catcatcagc ctggcccgcc tgccccttgt
    12361 caacagctac acacgtgtgc ccccactggt gtggaagctt ggatggtcac ccaaaccggg
    12421 aggggatttt ggcacagcat tccctgagat ccccgtggag ttcctccagg aaaaggaagt
    12481 ctttaaggag ttcatctacc gcatcaacac actaggctgg accagtcgta ctcagtttga
    12541 agaaacttgg gccaccctcc ttggtgtcct ggtgacgcag cccctcgtga tggagcagga
    12601 ggagagccca ccagaagaag acacagagag gacccagatc aacgtcctgg ccgtgcaggc
    12661 catcacctca ctggtgctca gtgcaatgac tgtgcctgtg gccggcaacc cagctgtaag
    12721 ctgcttggag cagcagcccc ggaacaagcc tctgaaagct ctcgacacca ggtttgggag
    12781 gaagctgagc attatcagag ggattgtgga gcaagagatt caagcaatgg tttcaaagag
    12841 agagaatatt gccacccatc atttatatca ggcatgggat cctgtccctt ctctgtctcc
    12901 ggctactaca ggtgccctca tcagccacga gaagctgctg ctgcagatca accccgagcg
    12961 ggagctgggg agcatgagct acaaactcgg ccaggtgtcc atacactccg tgtggctagg
    13021 gaacagcatc acacccctga gggaggagga atgggacgag gaagaggagg aggaggccga
    13081 cgcccctgca ccttcgtcac cacccacgtc tccagtcaac tccaggaaac accgggctgg
    13141 agttgacatc cactcctgtt cgcagttttt gcttgagttg tacagccgct ggatcctgcc
    13201 gtccagctca gccaggagga ccccggccat cctgatcagt gaggtggtca gatcccttct
    13261 agtggtctca gacttgttca ccgagcgcaa ccagtttgag ctgatgtatg tgacgctgac
    13321 agaactgcga agggtgcacc cttcagaaga cgagatcctc gctcagtacc tggtgcctgc
    13381 cacctgcaag gcagctgccg tccttgggat ggacaaggcc gtggcggagc ctgtcagccg
    13441 cctgctggag agcacgctca ggagcagcca cctgcccagc agggttggag ccctgcacgg
    13501 catcctctat gtgctggagt gcgacctgct ggacgacact gccaagcagc tcatcccggt
    13561 catcagcgac tatctcctct ccaacctgaa agggatcgcc cactgcgtga acattcacag
    13621 ccagcagcac gtactggtca tgtgtgccac tgcgttttac ctcattgaga actatcctct
    13681 ggacgtaggg ccggaatttt cagcatcaat aatacagatg tgtggggtga tgctgtctgg
    13741 aagtgaggag tccaccccct ccatcattta ccactgtgcc ctcagaggcc tggagcgcct
    13801 cctgctctct gagcagctct cccgcctgga tgcagaatcg ctggtcaagc tgagtgtgga
    13861 cagagtgaac gtgcacagcc cgcaccgggc catggcggct ctgggcctga tgctcacctg
    13921 catgtacaca ggaaaggaga aagtcagtcc gggtagaact tcagacccta atcctgcagc
    13981 ccccgacagc gagtcagtga ttgttgctat ggagcgggta tctgttcttt ttgataggat
    14041 caggaaaggc tttccttgtg aagccagagt ggtggccagg atcctgcccc agtttctaga
    14101 cgacttcttc ccaccccagg acatcatgaa caaagtcatc ggagagtttc tgtccaacca
    14161 gcagccatac ccccagttca tggccaccgt ggtgtataag gtgtttcaga ctctgcacag
    14221 caccgggcag tcgtccatgg tccgggactg ggtcatgctg tccctctcca acttcacgca
    14281 gagggccccg gtcgccatgg ccacgtggag cctctcctgc ttctttgtca gcgcgtccac
    14341 cagcccgtgg gtcgcggcga tcctcccaca tgtcatcagc aggatgggca agctggagca
    14401 ggtggacgtg aaccttttct gcctggtcgc cacagacttc tacagacacc agatagagga
    14461 ggagctcgac cgcagggcct tccagtctgt gcttgaggtg gttgcagccc caggaagccc
    14521 atatcaccgg ctgctgactt gtttacgaaa tgtccacaag gtcaccacct gcggtggaag
    14581 cggtgactac aaagacgatg acgacaagta aaacctctac ttccaatcgc accatcatca
    14641 ccatcaccat caccaccatg attacaagga tgacgacgat aagtgaggcg gccgccaact
    14701 tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
    14761 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
    14821 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
    14881 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
    14941 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
    15001 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
    15061 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
    15121 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
    15181 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcgggcgcc cccctcctca
    15241 cggcgagcga agcttgtcga gaagtactag aggatctcta gagcctgcag tctcgacaag
    15301 cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga ggttttactt
    15361 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    15421 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    15481 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    15541 gtatcttatc atgtctggat ctgatcactg cttgagccta ggagatccga accagataag
    15601 tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt acgacgctac
    15661 acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac tccatttcca
    15721 cccctcccag ttcccaacta ttttgtccgc ccacagcggg gcatttttct tcctgttatg
    15781 tttttaatca aacatcctgc caactccatg tgacaaaccg tcatcttcgg ctacttt
//
