LOCUS       sequence-220989-        4346 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: LOC5570372 NPFF receptor
FEATURES             Location/Qualifiers
     source          1..4346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2234..2368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2271..2290)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2325..2344
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2407..2424)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2578..3166)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2658..2677)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3337..4128,4129..4197))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3960..3979
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4198..4302)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(4342..4346,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tgaagaatgg agaagtccac agaaacgtgc ggcaacttgc
      841 accaaagaat gatggttctt gcaagttgtt gccttttcgt cagaatcagc aaaagatgaa
      901 cgttagcatc tacgattatc ccagagagat ctgggtgacc attccgtttt gggagatttt
      961 agtcaaagcc gccacctttc tgccattgat cacgtttggt atttttggca atatcgcatt
     1021 gctatgcatc atcttcacca ttcgagccct caggacgcca accaatatgt tgattgcaaa
     1081 tttggccgta gctgacttgg cgaccctgat tatctgtccg gtgatgttca tgctgcatga
     1141 tttctaccag aattatgtca tgggatgtgt tggatgcaag ctagaaggat ttcttgaagg
     1201 ttcgctgctc ataacttctg ttctgtgcct ttgtggaata agctacgata gattaacggc
     1261 aattgttttc ccgaagaaat ccagattgac gatgcgaggc gtaacagtta tcattgcctg
     1321 tgcttggctc gcgggatttt tacttgctct accgttaaca atttatcgaa actataagga
     1381 acggcagtgg aaaaattatt tggaaacatt ttgcgcagaa aatacgagtt ttcttccgca
     1441 atattggcat gtcctgattg gagctttggt ttggtttcca ctatctgtaa tgatttgctg
     1501 ttatagtttg atatttctta aacttgatcg atatcaaaaa agagtattaa atcgagagca
     1561 tccaatttca gtttcgtaca aaagaaaagt agctaagtgc ctatttgtag tattaattgt
     1621 gttcatcatc ctgcgaattc cattcaccac tttggttttc ataaggtatt atcgattttt
     1681 aaatgcgaac agtgatcaaa ttggaaaaga ttttcaaata ctttggtacg tgtcccacta
     1741 tttaatgttc ttaaatgccg ccatcaatcc actgatatat ggaatgacaa acgataattt
     1801 ccgaaaggct tacaataaga cgaaaatttg gaaatgcttc tggaaatcgg gaccagttaa
     1861 gaaggtggat cccaggaaaa aagaaccagc agtgaagatc atcgggcatc tccaacctcg
     1921 cggcccatgt ggaagtgata agtttgtgat cttttcagat tgggtcattc agcctaagca
     1981 agaagttcaa acttttgaat cacgagaaac taaatcgtca cagctaaaag ctacaacaga
     2041 tgatgtacag acgaaaagcg aacgaaaatc tttgtacgcc tgcaacttga ttctcgcaga
     2101 tcagtttcaa ggaaaacaaa ataatatcca atcagcagga ttcatttgag cggccgctag
     2161 actagtctag agaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     2221 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     2281 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     2341 catcaatgta tcttatcatg tctggatccc cgggtaccga gctcgaatta attcctcttc
     2401 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2461 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2521 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2581 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2641 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2701 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2761 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2821 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2881 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2941 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3001 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3061 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3121 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3181 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3241 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3301 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3361 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3421 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3481 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3541 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3601 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3661 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3721 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3781 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3841 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3901 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3961 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4021 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4081 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4141 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4201 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4261 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4321 gccacctgca agcttggctg tggaat
//
