LOCUS       sequence-221003-        4277 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: LOC5576189 CCHamide receptor
FEATURES             Location/Qualifiers
     source          1..4277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2165..2299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2202..2221)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2256..2275
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2338..2355)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2509..3097)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2589..2608)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3268..4059,4060..4128))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3891..3910
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4129..4233)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(4273..4277,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tggaactgtg gcgcttgata ccgaacggaa cggaatcggt
      841 tacggtggac gctcaacaga tattgggctt tccctcggtg atatcctcga cggtggagga
      901 catctttggc tccaatcgga tacagaagtc gattcggatg ctggaatcaa cggaggcgac
      961 ggcatttgga gatgtgtccg tggatcacgt ggatgaatac gaaccgtacg cggaccggtt
     1021 ggagacctac atagtgccgg tggtgtttgc catcatattc atcgtgggca ttctgggcaa
     1081 tggaaccctg gttatcatct ttctgcggca tcgagccatg cgaaacattc caaacaccta
     1141 cattttctcg ctggctttgg cagaccttct ggttatcttc gtgtgcgttc cgttggcgtc
     1201 gataatatac accctggaat cgtggccctg gggtgtcact ctgtgtcgcg tgaccgagtg
     1261 cttccgagaa atcagcatcg gcgtttcggt gttcaccctt actgccctgg cagccgatcg
     1321 atactgcgcc attgtcaacc ctttacgaaa gctgcagact cgcccagtca cggtcattgt
     1381 ggcggtgctc atctggttgc tggcgattgc tttcgccatt ccctcggcag ccatctcgga
     1441 cgtggtggtg gtgaaaattg agtccaacaa aaccatcgaa tattgttcac cgttcggaca
     1501 gccaagcgaa agcgctacag cttacaccaa atacaaaacc atcatcaatt cggtcattta
     1561 ctacttcctt ccgctggcca tcatcggcat cctgtacatc ctgatggcgt accgattgca
     1621 cgtcagcgcc cgggacatgc caggagagaa cgccggcccg cagagtcgtt cccaggccag
     1681 ggcccggaga cacgtggcac ggatggtgat taccttcgtg gtggttttca tcatctgctt
     1741 cctgccgcac cacgtgttcc agctgtggtt ccatctgaat ccgaactccc tgttcgagta
     1801 tgacgatttc tggcacatcc tgcggataac cggcttcctt ttgagttttc tcaactcatg
     1861 catcaacccg gtggccctgt actgcgtttc cggcgtattt cggcagcact tccaccggta
     1921 cctgtgctgt cggcagatgt ccgttcagcg gcgcatcggc atgtcctcgg ttggcaatgc
     1981 ttgcgaaacg agttttgtgt cgacgattcg acgctcgcag cataaccaca gcatccggaa
     2041 gtccctgtcg gttcactcgg tccagagcac gaagaggtga gcggccgcta gactagtcta
     2101 gagaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     2161 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     2221 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     2281 atcttatcat gtctggatcc ccgggtaccg agctcgaatt aattcctctt ccgcttcctc
     2341 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2401 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2461 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2521 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2581 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2641 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2701 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2761 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2821 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2881 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2941 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3001 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3061 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3121 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3181 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3241 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3301 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3361 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3421 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3481 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3541 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3601 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3661 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3721 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3781 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3841 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3901 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3961 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4021 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4081 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4141 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4201 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc
     4261 aagcttggct gtggaat
//
