LOCUS       sequence-221025-        9258 bp    DNA     circular SYN 14-APR-2025
DEFINITION  mouse BK channel alpha subunit expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9258)
  AUTHORS   Gruslova A, Semenov I, Wang B
  TITLE     An extracellular domain of the accessory beta1 subunit is required 
            for modulating BK channel voltage sensor and gate.
  JOURNAL   J Gen Physiol. 2012 Jan;139(1):57-67. doi: 10.1085/jgp.201110698. 
            Epub 2011 Dec 12.
  PUBMED    22155735
REFERENCE   2  (bases 1 to 9258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1085/jgp.201110698"; journalName: "J Gen Physiol"; date: 
            "2012-01"; volume: "139"; issue: "1"; pages: "57-67"
COMMENT     Sequence Label: BK alpha subunit
FEATURES             Location/Qualifiers
     source          1..9258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(3918..3936)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3919..3936)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3956..3973)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3962..4186
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4232..4660
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4319..4338)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4529..4550
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4669..4689)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4674..5003
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4854..4989
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4916..4935
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5070..5864
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5124..5143)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5734..5753
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6038..6159
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6075..6094)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6129..6148
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6208..6224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6208..6224)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6221..6243)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6232..6248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6256..6262,6263..6280,6281..6286))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6301..6322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6439..6456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6610..7198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6690..6709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7369..8160,8161..8229))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7992..8011
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8230..8334)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8409..8428)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8600..8979
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8980..9183
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9134..9154
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9228..9247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9228..9246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcttggtac ctcgagcccc tgcgcctgcc gcccattgct agctatggca aacggtggcg
       61 gcggaggcgg cggcagcagc ggcggcggcg gcggcggcgg cggaggcagc ggtcttagaa
      121 tgagcagcaa tatccacgcg aaccatctca gcctagacgc gtcctcctcc tcctcttcct
      181 cctcctcttc ttcttcttcc tcctcctctt cctcctcgtc ctcggtccac gagcccaaga
      241 tggatgcgct catcataccg gtgaccatgg aggtgccgtg cgacagccgg ggccaacgca
      301 tgtggtgggc tttcttggcc tcctccatgg tgactttctt cgggggcctc ttcatcatct
      361 tgctctggcg gacgctcaag tacctgtgga ccgtttgctg ccactgcggg ggcaagacga
      421 aggaggccca gaagataaac aatggctcca gccaggcaga tggtactctc aagccagtgg
      481 acgaaaaaga ggaggtggtg gcagccgagg tcggctggat gacatctgtg aaggactggg
      541 caggggtgat gatatccgcc cagacactga ctggcagagt cctggttgtg ttagtctttg
      601 ctctcagcat tggtgccctc gtaatatact tcatagactc gtcaaaccca atagaatcct
      661 gccagaattt ctacaaagat ttcacattac agatcgacat ggctttcaac gtgttcttcc
      721 tcctctactt tggcttgcgg tttattgcag ccaacgataa gctgtggttc tggctggaag
      781 tgaattcagt agtagatttc ttcacagtcc ctcctgtgtt tgtgtctgtg tacttaaaca
      841 gaagttggct tggcttgaga tttttaagag ctctcagact gatacagttt tcagagattt
      901 tgcagtttct gaatatcctt aaaacaagta actccatcaa gctggtgaat ctgctctcca
      961 tatttatcag cacgtggctg actgcagctg gattcatcca cttggtggag aattcagggg
     1021 acccatggga aaatttccaa aacaaccagg cacttacgta ctgggaatgt gtctacttac
     1081 tcatggtcac aatgtctaca gtgggttatg gggacgttta tgcaaaaacc acacttggac
     1141 gcctcttcat ggtcttcttc atcctcgggg gactggccat gtttgcctcg tacgtccctg
     1201 aaatcataga gttaatagga aaccgcaaga aatacggggg ctcctatagc gcggttagtg
     1261 gaagaaagca cattgtagtc tgtggacaca ttactctgga gagtgtctct aacttcctga
     1321 aggactttct gcacaaggac cgggatgatg tcaacgtgga gattgtcttt cttcacaaca
     1381 tctcccctaa ccttgagctt gaggctctgt tcaaacggca tttcactcag gtggagtttt
     1441 atcagggctc tgtcctcaat ccacatgatc ttgccagagt caagatagag tcagcagatg
     1501 catgcctgat ccttgccaat aagtattgcg ctgacccgga tgcagaagat gcctccaaca
     1561 tcatgagagt gatctccatc aaaaactacc acccaaagat caggatcatc actcagatgc
     1621 tgcagtatca caacaaggcc catctgctca acatccccag ctggaactgg aaagagggtg
     1681 atgacgcaat atgccttgca gagctcaagt tgggtttcat agcccagagc tgtctggctc
     1741 aaggcctctc cacaatgctt gccaatctct tctctatgag gtcattcata aagattgagg
     1801 aagacacatg gcagaaatac tacttggaag gagtctccaa tgaaatgtac acagaatatc
     1861 tctccagtgc cttcgtgggt ctgtccttcc ctactgtttg tgagctgtgt tttgtgaagc
     1921 ttaagctcct gatgatagcc attgagtaca agtctgccaa cagagagagc cgaagccgaa
     1981 agcgaatatt aattaaccct gggaaccacc ttaagatcca agaaggtact ttaggatttt
     2041 tcatcgcaag tgatgccaaa gaagttaaaa gggcattttt ttactgcaag gcctgtcatg
     2101 atgacgtcac agatcccaaa agaattaaaa aatgtggctg caggcggctt gaagatgagc
     2161 agccgccaac cctgtcacca aaaaaaaaac aacgtaatgg gggcatgagg aactcgccca
     2221 acacctcccc gaagctgatg aggcatgacc ccttgttaat tcctggcaat gatcagattg
     2281 acaacatgga ctccaatgtg aaaaagtacg actccactgg aatgtttcac tggtgtgcac
     2341 ccaaggagat tgagaaagtc atcttgactc gaagtgaagc tgccatgact gtcctgagtg
     2401 gccatgtcgt agtctgcatc tttggggatg tcagctcagc cctgattggc ctccggaacc
     2461 tggtgatgcc acttcgtgct agcaactttc actatcatga gctcaaacac attgtgtttg
     2521 tgggctccat tgagtacctc aagagggagt gggaaacact gcacaacttc ccgaaagtgt
     2581 ccatattgcc tggtacacca ttaagtcggg ctgatttaag ggctgtcaac atcaacctct
     2641 gtgacatgtg cgttatcctg tcagccaatc agaataatat tgatgatact tcgcttcagg
     2701 acaaggaatg catcttggcg tcactcaaca tcaaatctat gcagtttgat gacagcatcg
     2761 gggtcttgca ggctaattcc caaggattca cacctcctgg aatggacaga tcatcacccg
     2821 acaacagccc agtgcacggg atgttacgcc agccgtccat cacaactggg gtcaacattc
     2881 ccatcatcac ggaactcgct aagccgggca agttgccttt ggtatcagtc aatcaggaaa
     2941 aaaacagtgg gacgcacatt ctaatgataa cggagttggt gaatgatacc aatgttcagt
     3001 ttttggacca agacgatgac gatgaccctg acacagagct gtacctcaca cagccctttg
     3061 cttgtgggac agcatttgcc gtcagcgtcc tggactcact catgagcgcg acatacttca
     3121 atgacaatat cctcacccta atacggaccc tggtgacagg aggagccaca ccagagctcg
     3181 aggctctaat agctgaggag aatgcacttc gaggaggcta cagcactccg cagacattgg
     3241 ccaacaggga ccgttgccga gtggcccagt tagccctgtt agatggtccc tttgcagact
     3301 taggggatgg tggttgttat ggtgatctgt tctgcaaagc tctgaaaaca tataatatgc
     3361 tttgttttgg aatttaccgg ctgagagatg cccacctcag cacccccagc cagtgtacaa
     3421 aaaggtacgt catcaccaat cctccctacg agtttgagct cgtaccaaca gacctgatct
     3481 tctgcctgat gcagtttgac cacaacgctg gccaatcccg ggccagtctg tctcattcct
     3541 cccactcctc acagtcgtcc agtaagaaga gctcctccgt ccactccatc ccgtccacag
     3601 caaatcggcc gaaccggccc aagtccaggg agtcccgcga caaacagaac agaaaagaaa
     3661 tggtttacag atgagccgga taatgcctat cccagaaaca ttcaaatcaa gcccatgagt
     3721 acccacatgg ctaaccagat caaccaatat aaatccacaa gcagcctgat ccctccaatc
     3781 agagaagttg aagatgaatg ttgactccca ggagaccaga actatttttt taaagcctga
     3841 caaacttcat aaatggtgac gtgacttttc ttcctcttac atgctgaatc actggtggaa
     3901 attctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
     3961 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4021 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4081 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4141 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4201 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4321 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4381 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4441 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4501 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4561 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4621 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     4681 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4741 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4801 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4861 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4921 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     4981 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     5041 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     5101 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     5161 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     5221 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     5281 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5341 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     5401 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5461 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     5521 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     5581 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     5641 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     5701 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     5761 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5821 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     5881 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     5941 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6001 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     6061 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6121 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     6181 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6241 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6301 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6361 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6421 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6481 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6541 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6601 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6661 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6721 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6781 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6841 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6901 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6961 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7021 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7081 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7141 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7201 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7261 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7321 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7381 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7441 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7501 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7561 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7621 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7681 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7741 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7801 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7861 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7921 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7981 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8041 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8101 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8161 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8221 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8281 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8341 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     8401 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     8461 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     8521 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     8581 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     8641 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     8701 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     8761 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     8821 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     8881 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     8941 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     9001 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     9061 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     9121 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     9181 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
     9241 tatagggaga cccaagct
//
