LOCUS       sequence-221036-        3917 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: AAEL012385 MIP R
FEATURES             Location/Qualifiers
     source          1..3917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    1805..1939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1842..1861)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1896..1915
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1978..1995)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2149..2737)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2229..2248)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2908..3699,3700..3768))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3531..3550
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3769..3873)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(3913..3917,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tggagctact tcgactaata ccatcgaaat actcggcatc
      841 ttcttcatca gcgaagcgac tactacatgc gcaacaatcc gttgcccgta gttgctgcct
      901 tcgtgatgca atacgcatcg catcgttcat ccatcttcgg agcagctact ctgagagcaa
      961 cagtccgttg acggtagcca ttgccttcga ggtgttagcc cttggtatcg attttcgtca
     1021 tcatcgtcga agcggatggc aacaatccat tgcccgaagt cattgccttc gagatggtac
     1081 aacgatacaa actctccctc tcttcaaaag ggtttcgtcg tgcgcaacag tccgatgccc
     1141 gaaacctcga cattacagga agttacaaca tcgtcaccgt cgagaatcgt tgaaacggtc
     1201 cctgccagcc atcgactatt gctttcgaga ttcaagtcgt catctttact acccatattg
     1261 ccgaagcggc caccgcaaca gcgatgaatg ccttcgggat acatttggac attctcatca
     1321 tcgaaaccat caaatcattc gaaattcatc gctttatgtt gcccaatacg atcctttcac
     1381 ggtgggcggc gcatttaagc tcaaacgaag atcatattct atgcgaacat catcccattc
     1441 aacatccaca acaacgagca tcaaaatcac cgatcatcaa agcgacaacg accagttttg
     1501 ccatttatct tccatcatct cctcaacccg atcgcgcaag gtgggcggca aatccttatc
     1561 gtcgttcatc aaatcatcaa cttccatact atcaaggtgg gcggcaaatc accaaccgtc
     1621 attatcatca tcactactag tatcaacaaa tcaatctaat cctacatgcc tactggaaaa
     1681 agcagcgaac atcaattcat ctgatcgcat acatcagtga gcggccgcta gactagtcta
     1741 gagaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     1801 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     1861 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     1921 atcttatcat gtctggatcc ccgggtaccg agctcgaatt aattcctctt ccgcttcctc
     1981 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2041 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2101 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2161 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2221 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2281 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2341 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2401 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2461 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2521 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2581 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2641 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2701 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2761 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2821 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2881 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2941 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3001 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3061 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3121 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3181 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3241 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3301 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3361 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3421 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3481 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3541 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3601 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3661 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3721 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3781 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3841 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc
     3901 aagcttggct gtggaat
//
