LOCUS       sequence-221166-        8553 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of exogenous LAP tagged MPS1 WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Nijenhuis W, von Castelmur E, Littler D, De Marco V, Tromer E, 
            Vleugel M, van Osch MH, Snel B, Perrakis A, Kops GJ
  TITLE     A TPR domain-containing N-terminal module of MPS1 is required for 
            its kinetochore localization by Aurora B.
  JOURNAL   J Cell Biol. 2013 Apr 15;201(2):217-31. doi: 10.1083/jcb.201210033. 
            Epub 2013 Apr 8.
  PUBMED    23569217
REFERENCE   2  (bases 1 to 8553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201210033"; journalName: "J Cell Biol"; date: 
            "2013-04-15- 15"; volume: "201"; issue: "2"; pages: "217-31"
COMMENT     Sequence Label: pCDNA5-LAP-MPS1-WT
FEATURES             Location/Qualifiers
     source          1..8553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        155..534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        535..738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     689..709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    740..758
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    761..779
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     789..813
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      938..947
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(944..946,947..949,950..1660)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(989..1010)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1250..1269)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1597..1618
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1706..1726
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1739..1783
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(4425..4442)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4431..4655
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4788..4807)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4939..4986
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4994..6016
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4994..6016
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6146..6267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6183..6202)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6237..6256
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6316..6332)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6316..6332)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6329..6351)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6340..6356
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6364..6370,6371..6388,6389..6394))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6409..6430
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6547..6564)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6718..7306)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6798..6817)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7477..8268,8269..8337))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8100..8119
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8338..8442)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8517..8536)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
       61 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
      121 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
      181 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      241 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      301 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      361 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      421 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      481 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      541 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      601 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      661 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      721 gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat
      781 cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      841 gacctccata gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag
      901 cttggtaccg agctcggatc cgctagcgct accggtcgcc accatggtga gcaagggcga
      961 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1021 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1081 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
     1141 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1201 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1261 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1321 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1381 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1441 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1501 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     1561 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1621 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggagggg atcggtggag
     1681 ctccaccggt ggcggccgct ctagagagaa tctttatttt cagggcgccg ccaaattcaa
     1741 agaaaccgct gctgctaaat tcgaacgcca gcacatggac agcggaggtg gaggtactag
     1801 tctcgagatg gaatccgagg atttaagtgg cagagaattg acaattgatt ccataatgaa
     1861 caaagtgaga gacattaaaa ataagtttaa aaatgaagac cttactgatg aactaagctt
     1921 gaataaaatt tctgctgata ctacagataa ctcgggaact gttaaccaaa ttatgatgat
     1981 ggcaaacaac ccagaggact ggttgagttt gttgctcaaa ctagagaaaa acagtgttcc
     2041 gctaagtgat gctcttttaa ataaattgat tggtcgttac agtcaagcaa ttgaagcgct
     2101 tcccccagat aaatatggcc aaaatgagag ttttgctaga attcaagtga gatttgctga
     2161 attaaaagct attcaagagc cagatgatgc acgtgactac tttcaaatgg ccagagcaaa
     2221 ctgcaagaaa tttgcttttg ttcatatatc ttttgcacaa tttgaactgt cacaaggtaa
     2281 tgtcaaaaaa agtaaacaac ttcttcaaaa agctgtagaa cgtggagcag taccactaga
     2341 aatgctggaa attgccctgc ggaatttaaa cctccaaaaa aagcagctgc tttcagagga
     2401 ggaaaagaag aatttatcag catctacggt attaactgcc caagaatcat tttccggttc
     2461 acttgggcat ttacagaata ggaacaacag ttgtgattcc agaggacaga ctactaaagc
     2521 caggttttta tatggagaga acatgccacc acaagatgca gaaataggtt accggaattc
     2581 attgagacaa actaacaaaa ctaaacagtc atgcccattt ggaagagtcc cagttaacct
     2641 tctaaatagc ccagattgtg atgtgaagac ggacgattca gttgtacctt gttttatgaa
     2701 aagacaaacc tctagatcag aatgccgaga tttggttgtg cctggatcta aaccaagtgg
     2761 aaatgattcc tgtgaattaa gaaatttaaa gtctgttcaa aatagtcatt tcaaggaacc
     2821 tctggtgtca gatgaaaaga gttctgaact tattattact gattcaataa ccctgaagaa
     2881 taaaacggaa tcaagtcttc tagctaaatt agaagaaact aaagagtatc aagaaccaga
     2941 ggttccagag agtaaccaga aacagtggca atctaagaga aagtcagagt gtattaacca
     3001 gaatcctgct gcatcttcaa atcactggca gattccggag ttagcccgaa aagttaatac
     3061 agagcagaaa cataccactt ttgagcaacc tgtcttttca gtttcaaaac agtcaccacc
     3121 aatatcaaca tctaaatggt ttgacccaaa atctatttgt aagacaccaa gcagcaatac
     3181 cttggatgat tacatgagct gttttagaac tccagttgta aagaatgact ttccacctgc
     3241 ttgtcagttg tcaacacctt atggccaacc tgcctgtttc cagcagcaac agcatcaaat
     3301 acttgccact ccacttcaaa atttacaggt tttagcatct tcttcagcaa atgaatgcat
     3361 ttcggttaaa ggaagaattt attccatatt aaagcagata ggaagtggag gttcaagcaa
     3421 ggtatttcag gtgttaaatg aaaagaaaca gatatatgct ataaaatatg tgaacttaga
     3481 agaagcagat aaccaaactc ttgatagtta ccggaacgaa atagcttatt tgaataaact
     3541 acaacaacac agtgataaga tcatccgact ttatgattat gaaatcacgg accagtacat
     3601 ctacatggta atggagtgtg gaaatattga tcttaatagt tggcttaaaa agaaaaaatc
     3661 cattgatcca tgggaacgca agagttactg gaaaaatatg ttagaggcag ttcacacaat
     3721 ccatcaacat ggcattgttc acagtgatct taaaccagct aactttctga tagttgatgg
     3781 aatgctaaag ctaattgatt ttgggattgc aaaccaaatg caaccagata caacaagtgt
     3841 tgttaaagat tctcaggttg gcacagttaa ttatatgcca ccagaagcaa tcaaagatat
     3901 gtcttcctcc agagagaatg ggaaatctaa gtcaaagata agccccaaaa gtgatgtttg
     3961 gtccttagga tgtattttgt actatatgac ttacgggaaa acaccatttc agcagataat
     4021 taatcagatt tctaaattac atgccataat tgatcctaat catgaaattg aatttcccga
     4081 tattccagag aaagatcttc aagatgtgtt aaagtgttgt ttaaaaaggg acccaaaaca
     4141 gaggatatcc attcctgagc tcctggctca tccatatgtt caaattcaaa ctcatccagt
     4201 taaccaaatg gccaagggaa ccactgaaga aatgaaatat gttctgggcc aacttgttgg
     4261 tctgaattct cctaactcca ttttgaaagc tgctaaaact ttatatgaac actatagtgg
     4321 tggtgaaagt cataattctt catcctccaa gacttttgaa aaaaaaaggg gaaaaaaatg
     4381 agtcgagcat gcatctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     4441 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     4501 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     4561 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     4621 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     4681 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4741 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4801 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4861 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     4921 tgatggttca cgtacctaga agttcctatt ccgaagttcc tattctctag aaagtatagg
     4981 aacttccttg gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     5041 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     5101 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     5161 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     5221 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     5281 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     5341 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     5401 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     5461 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     5521 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     5581 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     5641 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     5701 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     5761 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     5821 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     5881 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     5941 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     6001 gagggcaaag gaatagcacg tactacgaga tttcgattcc accgccgcct tctatgaaag
     6061 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     6121 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     6181 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6241 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     6301 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6361 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6421 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6481 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6541 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6601 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6661 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6721 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6781 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6841 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6901 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6961 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7021 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7081 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7141 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7201 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7261 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7321 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7381 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7441 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7501 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7561 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7621 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7681 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7741 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7801 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7861 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7921 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7981 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8041 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8101 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8161 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8221 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8281 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8341 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8401 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8461 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     8521 atctgctctg atgccgcata gttaagccag tat
//
