LOCUS       sequence-221170-        7948 bp    DNA     circular SYN 15-APR-2025
DEFINITION  protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Jelluma N, Brenkman AB, McLeod I, Yates JR 3rd, Cleveland DW, Medema
            RH, Kops GJ
  TITLE     Chromosomal instability by inefficient Mps1 auto-activation due to a
            weakened mitotic checkpoint and lagging chromosomes.
  JOURNAL   PLoS One. 2008 Jun 11;3(6):e2415. doi: 10.1371/journal.pone.0002415.
  PUBMED    18545697
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2008-06-11"; volume: "3(6):e2415. doi"; pages: " 
            10.1371/journal.pone.0002415"
COMMENT     Sequence Label: pFastBac-GST-Mps1-KD
FEATURES             Location/Qualifiers
     source          1..7948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        151..242
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     167..184
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             285..938
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     896..918
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             945..962
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     polyA_signal    3704..3838
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3741..3760)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3795..3814
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3867..4032
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4216..4670
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4303..4322)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4512..4533
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4696..4800
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4801..4869,4870..5661)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5019..5038)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5832..6420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6321..6340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6574..6591
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  6725..6949
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(7016..7549)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7719..7724,7725..7741,7742..7747))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct
       61 cggttttctg gaaggcgagc atcgtttgtt cgcccaggac tctagctata gttctagtgg
      121 ttggctacgt atactccgga atattaatag atcatggaga taattaaaat gataaccatc
      181 tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa
      241 atattccgga ttattcatac cgtcccacca tcgggcgcgg atccatgtcc cctatactag
      301 gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag
      361 aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt
      421 ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa
      481 cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc
      541 caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg
      601 tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc
      661 tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg
      721 atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg
      781 acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta
      841 tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct
      901 ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt ccgcgtggat
      961 cgaattcaaa ggcctacgtc gagatggaat ccgaggattt aagtggcaga gaattgacaa
     1021 ttgattccat aatgaacaaa gtgagagaca ttaaaaataa gtttaaaaat gaagacctta
     1081 ctgatgaact aagcttgaat aaaatttctg ctgatactac agataactcg ggaactgtta
     1141 accaaattat gatgatggca aacaacccag aggactggtt gagtttgttg ctcaaactag
     1201 agaaaaacag tgttccgcta agtgatgctc ttttaaataa attgattggt cgttacagtc
     1261 aagcaattga agcgcttccc ccagataaat atggccaaaa tgagagtttt gctagaattc
     1321 aagtgagatt tgctgaatta aaagctattc aagagccaga tgatgcacgt gactactttc
     1381 aaatggccag agcaaactgc aagaaatttg cttttgttca tatatctttt gcacaatttg
     1441 aactgtcaca aggtaatgtc aaaaaaagta aacaacttct tcaaaaagct gtagaacgtg
     1501 gagcagtacc actagaaatg ctggaaattg ccctgcggaa tttaaacctc caaaaaaagc
     1561 agctgctttc agaggaggaa aagaagaatt tatcagcatc tacggtatta actgcccaag
     1621 aatcattttc cggttcactt gggcatttac agaataggaa caacagttgt gattccagag
     1681 gacagactac taaagccagg tttttatatg gagagaacat gccaccacaa gatgcagaaa
     1741 taggttaccg gaattcattg agacaaacta acaaaactaa acagtcatgc ccatttggaa
     1801 gagtcccagt taaccttcta aatagcccag attgtgatgt gaagacggac gattcagttg
     1861 taccttgttt tatgaaaaga caaacctcta gatcagaatg ccgagatttg gttgtgcctg
     1921 gatctaaacc aagtggaaat gattcctgtg aattaagaaa tttaaagtct gttcaaaata
     1981 gtcatttcaa ggaacctctg gtgtcagatg aaaagagttc tgaacttatt attactgatt
     2041 caataaccct gaagaataaa acggaatcaa gtcttctagc taaattagaa gaaactaaag
     2101 agtatcaaga accagaggtt ccagagagta accagaaaca gtggcaatct aagagaaagt
     2161 cagagtgtat taaccagaat cctgctgcat cttcaaatca ctggcagatt ccggagttag
     2221 cccgaaaagt taatacagag cagaaacata ccacttttga gcaacctgtc ttttcagttt
     2281 caaaacagtc accaccaata tcaacatcta aatggtttga cccaaaatct atttgtaaga
     2341 caccaagcag caataccttg gatgattaca tgagctgttt tagaactcca gttgtaaaga
     2401 atgactttcc acctgcttgt cagttgtcaa caccttatgg ccaacctgcc tgtttccagc
     2461 agcaacagca tcaaatactt gccactccac ttcaaaattt acaggtttta gcatcttctt
     2521 cagcaaatga atgcatttcg gttaaaggaa gaatttattc catattaaag cagataggaa
     2581 gtggaggttc aagcaaggta tttcaggtgt taaatgaaaa gaaacagata tatgctataa
     2641 aatatgtgaa cttagaagaa gcagataacc aaactcttga tagttaccgg aacgaaatag
     2701 cttatttgaa taaactacaa caacacagtg ataagatcat ccgactttat gattatgaaa
     2761 tcacggacca gtacatctac atggtaatgg agtgtggaaa tattgatctt aatagttggc
     2821 ttaaaaagaa aaaatccatt gatccatggg aacgcaagag ttactggaaa aatatgttag
     2881 aggcagttca cacaatccat caacatggca ttgttcacag tgatcttaaa ccagctaact
     2941 ttctgatagt tgatggaatg ctaaagctaa ttgcttttgg gattgcaaac caaatgcaac
     3001 cagatacaac aagtgttgtt aaagattctc aggttggcac agttaattat atgccaccag
     3061 aagcaatcaa agatatgtct tcctccagag agaatgggaa atctaagtca aagataagcc
     3121 ccaaaagtga tgtttggtcc ttaggatgta ttttgtacta tatgacttac gggaaaacac
     3181 catttcagca gataattaat cagatttcta aattacatgc cataattgat cctaatcatg
     3241 aaattgaatt tcccgatatt ccagagaaag atcttcaaga tgtgttaaag tgttgtttaa
     3301 aaagggaccc aaaacagagg atatccattc ctgagctcct ggctcatcca tatgttcaaa
     3361 ttcaaactca tccagttaac caaatggcca agggaaccac tgaagaaatg aaatatgttc
     3421 tgggccaact tgttggtctg aattctccta actccatttt gaaagctgct aaaactttat
     3481 atgaacacta tagtggtggt gaaagtcata attcttcatc ctccaagact tttgaaaaaa
     3541 aaaggggaaa aaaatgagtc gagcatgcgg taccaagctt gtcgagaagt actagaggat
     3601 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     3661 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     3721 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3781 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg
     3841 atcactgctt gagcctagga gatccgaacc agataagtga aatctagttc caaactattt
     3901 tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc
     3961 actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt
     4021 tgtccgccca cagcggggca tttttcttcc tgttatgttt ttaatcaaac atcctgccaa
     4081 ctccatgtga caaaccgtca tcttcggcta ctttttctct gtcacagaat gaaaattttt
     4141 ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat atcaacgctt atttgcagcc
     4201 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4261 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4321 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4381 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4441 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4501 cgttcttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4561 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4621 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     4681 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4741 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4801 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4861 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4921 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4981 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5041 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5101 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5161 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5221 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5281 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5341 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5401 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5461 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5521 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5581 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5641 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5701 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5761 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5821 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5881 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5941 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6001 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6061 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6121 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6181 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6241 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6301 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6361 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6421 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6481 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6541 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6601 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatagac
     6661 cagccgcgta acctggcaaa atcggttacg gttgagtaat aaatggatgc cctgcgtaag
     6721 cgggtgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac tatgacaata
     6781 aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg ctgtgaaaaa
     6841 gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt gcaaattgcc
     6901 cgtcgtatta aagaggggcg tggccaaggg catggtaaag actatattcg cggcgttgtg
     6961 acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg
     7021 tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat
     7081 agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa
     7141 gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt
     7201 gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg
     7261 gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga
     7321 tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt
     7381 aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga
     7441 cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg
     7501 ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     7561 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt
     7621 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     7681 tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt
     7741 atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg
     7801 gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct
     7861 ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg
     7921 atctgccctg gcttcaggag atcggaag
//
