LOCUS       sequence-221195-         823 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  GFP tethered to two repeats of the Gcn5 consensus motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: GFP-X2
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     152..168
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     152..168
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(218..246)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 agtttgctgt cttgctatcn ngtataaata gacctgcaat tattaatctt ttgtttcctc
       61 gtcattgttc tcgttccctt tcttccttgt ttctttttct gcacaatatt tcaagctata
      121 ccaagcatac aatcaactcc aagctggccg ctctagaact agtggatccc ccgggctgca
      181 ggaattcatg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      241 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      301 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      361 aacacttgtc actactttca cttatggtgt tcaatgcttt tcaagatacc cagatcatat
      421 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      481 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
      541 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      601 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
      661 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
      721 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt nnncngncaa
      781 ccattacctg tccacacaat ctgccctttc gaangatccc aac
//
