LOCUS       sequence-221691-        7713 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 683, 
            Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 7713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: mIFP12-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..7713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1512
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     protein_bind    3367..3378
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    4441..4562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4478..4497)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4532..4551
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4569..5024)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4706..4727)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4918..4937
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5051..5155
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5157..5514
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5157..5348
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5180..5200)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5365..5500
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5549..6343
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5549..6343
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5603..5622)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6213..6232
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6531..6550)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6575..6622
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6951..7539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7440..7459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgtcggt accgctgact acctcagcat tcggccacgc gtttctggct
      601 aactgtgaac gcgagcagat ccacctggcg ggctccattc agccgcacgg tatcctgctg
      661 gctgtgaaag agccggacaa cgtggtgatc caggcttcta ttaacgctgc ggagttcctg
      721 aacaccaact ctgttgttgg ccgtccgctg cgtgacctgg gcggcgatct gcctttgcag
      781 atcctgccgc acctgaacgg cccgctgcac ctggctccga tgaccctgcg ttgtaccgtg
      841 ggttctccgc cgcgtcgtgt ggactgtacc attcatcgtc cgtctaacgg cggcctgatc
      901 gtagaactgg aaccagcaac caagaccact aacattgcgc cggctctgga cggtgcgttt
      961 catcgtatca cttcttcatc ctccctgatg ggcctgtgtg acgaaaccgc gactattatc
     1021 cgtgagatta ctggctacga ccgtgtgatg gtagtacgtt tcgatgaaga gggtaatggc
     1081 gaaattctgt ccgaacgtcg tcgtgcggac ctggaagcgt tcctgggtaa ccgctacccg
     1141 gcgtctacta ttccgcagat cgctcgtcgc ctgtacgaac ataaccgtgt tcgcctgctg
     1201 gtagatgtga actatactcc ggttccgcta cagccgcgca tcagcccgct gaacggtcgt
     1261 gatctggata tgtccctgtc ttgcctgcgc tctatgtccc cgatccacca gaaatacatg
     1321 caggacatgg gcgttggcgc gaccctggtt tgctctctga tggtgtctgg tcgtctgtgg
     1381 ggtctgatcg cttgccacca ctacgaaccg cgcttcgttc cgttccacat tcgcgctgct
     1441 ggcgaagcgc tggcggaaac ttgtgcgatc cgcatcgcga cgctggagag ctttgcacag
     1501 tctcagtcca aaggtggcgg aagcggcggc ggaagcggtg gcggaagttc cggactcaga
     1561 tctggcagcg gtggaggctt ggaggagccg gaggccgcga cgcggacagc cgcggccgtg
     1621 gattgcaaag acagacccgg gttcccagtg aagaggttaa tacaagcccg cttgcccttc
     1681 aaacgcctga accttgttcc caaggagaaa gttgaggaag acacatcacc aaaagcagct
     1741 gtggaaagca aagtcccgga tctacaattg tctttgggca cttttgaaag ccagtgtcac
     1801 acaggctctc acgtaggctt gagtacaaag ttggtcggtg gccagggccc cattgatagc
     1861 ttcttgaggg caacaatcaa gccagtaccg agtgtggtca ttatcgacct gacggagaac
     1921 tgcagtgaca ttccagacag ccctgagggc cacagtgaac taagtcctga cactgctggt
     1981 gtggtcacca cagtggaagg agctgccaag cagcaggagc actctgcagc agagctgtgt
     2041 ctcctagaga ccccttcaga catcacttgt cacatggaag aggagcctgg gagtccagga
     2101 gacccaaaga ggacgggtga ctgtcaggct ggttcactac agagctgtcc ggagctgacc
     2161 ccaggttcta ggacatgccc caccaaggag ttatcaagct ggagcaaggc aggggatctc
     2221 ctatttatag agaaagtgcc tgtggtggtg ctagaggaca tcctggccac taagccctcc
     2281 attgcctctc ttcccatgat gtccctggat aggagtgtca cttcagagag tgaaatactg
     2341 gagtcctgcc ccgaggatga ctccatactg agccactcct ccaccaactc ctcttctcca
     2401 accagctctc ctgagggacc atccacaccc ccagaacatc ggggtgggag aagctccccc
     2461 tccaccccag cctgcagagt tgccaagaac tttgtcaaag gctccacaga gaagggcagg
     2521 agcaaactgc acagagatag agaacagcag agggaagaga aggaaaagct gagggaagag
     2581 atacgacgag ccaaggagga ggcacggaag aagaaggagg aggagaagga gctgaaggaa
     2641 aaggagcgga gggaaaagcg ggagaaagat gagaaggaga aggcggagaa gcagcggcta
     2701 aaggaggaaa aacgcaagga gcggcaggaa gctctggagg ctaaactgga ggagaagagg
     2761 aagaaggagg aagagaagcg gttacgggag gaagagaagc ggttacggga agaagagaag
     2821 cgcattaagg cagaaaaggc agagatcact cgattcttcc agaaaccgaa gactccgcag
     2881 gctcccaaga ccctggccgg ttcctgtggt aagtttgccc cctttgaaat caaagaacac
     2941 atggtgctgg ctccacggtg ccgagctgcc ttggatcaag acctgtgcga tcagctggac
     3001 cagctcctgc agcagcagag tgtggcaagc accttcctca gtgacctaaa aagccggctg
     3061 cccttgagat cggggcccac ccgggtctgt ggccatgaca cagacatcat gaacagggat
     3121 gtggtcattg tggagagcag caaggtggac ggtgtgtctg aaaggaagaa gtttggccgc
     3181 atgaagcttc tgcagttctc agagaaccac cggccagcat actgggggac gtggaacaag
     3241 aagacagcca tcatccgccc ccggaacccc tgggcccagg acaaagacct tcttgactat
     3301 gaggtagaca gtgacgatga gtgggaagag gaggaacctg gggagtcttt gtcccacagt
     3361 gagggggatg aggatgacga tgtgggggaa gatgaggatg aggacgatgg cttctttgtg
     3421 ccccatgggt acttatctga agatgaaggc gtgactgagg agtgtgcaga ccctgagaac
     3481 cacaaagtac accagaagct gaaggccaag gagtgggatg agctcctagc caagggcaag
     3541 cgctttcgtg tgctgcagcc tgtgcacgtg ggatgtgtat gggcatcaga ggcagccaac
     3601 tgcacgagtt ccgacttgaa gctgctgcag cagttcactg catgcctgct ggatgtggca
     3661 tcccctgatg agccagaacc cggggcctcc aggagggaaa agagggacca gcatatcctg
     3721 gcccagttgc ttccgctgct acatggcaat gtgaatggga gcaaggtgat catccatgaa
     3781 ttccaggagc aatgccgcag gggcctgctg acccttccaa gtcccacccc acacctgcag
     3841 atgcccaact tggaggatgc tgttgctgtg ccatccaagg ccaggctgaa gcgccttatc
     3901 tctgagaact ctgcctatga gaagcggccc aacttccgta tgtgctggta tgtgcaccca
     3961 gaggtgctca agagctttgg ccaggagtgc ctgccagtgc cctgccagtg gacctacatc
     4021 accaccatgc cctcagcacc cagggaagac agtggcagtg cttctactga ggggcctggc
     4081 cagagcacac ctatgctact caagcggaag ccagcggcca ccatgtgcat cacgcagttt
     4141 atgaagaagc ggcgctatga tggacaggtt gggtctgggg acatggatgg cttccaggca
     4201 gacacagaag aggatgaaga ggatgacaca gactgtatga tcatagatgt gccagatgtt
     4261 gggagtgacg tgtcagaagc tcccatacct gctcccacac tttgcaagtg aggatccacc
     4321 ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta
     4381 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     4441 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4501 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4561 taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     4621 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     4681 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     4741 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     4801 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     4861 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     4921 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     4981 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     5041 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5101 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
     5161 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5221 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5281 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5341 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5401 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5461 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
     5521 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     5581 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5641 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5701 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
     5761 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     5821 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     5881 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     5941 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6001 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6061 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6121 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6181 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6241 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6301 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6361 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     6421 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6481 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
     6541 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     6601 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     6661 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     6721 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     6781 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
     6841 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6901 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6961 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7021 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7081 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7141 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7201 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7261 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7321 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7381 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7441 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7501 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7561 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7621 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg
     7681 gtcattagtt catagcccat atatggagtt ccg
//
