LOCUS       sequence-222708-        7846 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Non-Integrating Foamy Virus pol-expressing helper plasmid with 
            mutations in the DDE catalyic core motif of the integrase sequence, 
            remaining as unintegated episomes in transduced cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Deyle DR, Li Y, Olson EM, Russell DW
  TITLE     Nonintegrating foamy virus vectors.
  JOURNAL   J Virol. 2010 Sep;84(18):9341-9. doi: 10.1128/JVI.00394-10. Epub 
            2010 Jun 30.
  PUBMED    20592072
REFERENCE   2  (bases 1 to 7846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.00394-10"; journalName: "J Virol"; date: "2010-09"; 
            volume: "84"; issue: "18"; pages: "9341-9"
COMMENT     Sequence Label: pCiPS-NIFV
FEATURES             Location/Qualifiers
     source          1..7846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     485..505
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     531..555
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          672..768
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    4985..5119
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5009..5028)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5063..5082
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5230..5246)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5230..5246)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5243..5265)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5254..5270
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5278..5284,5285..5302,5303..5308))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5323..5344
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5461..5478)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5632..6220)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5712..5731)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6391..7182,7183..7251))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7014..7033
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7252..7356)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7424..7442
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7480..7502)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7602..7621
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7815..7837
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7829..7846
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7830..7846
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tcgaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg gccgcaaaaa tcaaggagga
      841 tataatcttc gaccccgtac ttaccaacct caaaggtacg gaggaggacg tggacgaaga
      901 tggaacgata atactaacaa tcaagagtcc agaccatcag atcaaggttc tcaaactcct
      961 aggccaaatc aagcaggctc tggggtgcgt ggcaatcagt cacaaactcc cagaccagct
     1021 gctggtcgcg gaggaagagg taaccacaac cgaaaccaac gatcatccgg tgctggtgac
     1081 tcacgcgctg tcaataccgt gacacagagt gccacgtcct ccacagatga atcctcttca
     1141 gctgttacag ccgcttccgg cggagatcaa agggactaaa ttgttagccc actgggattc
     1201 aggggcaaca ataacttgta ttcctgaaag ttttttagaa gatgaacaac ctattaaaaa
     1261 gactttaata aaaacaattc atggagaaaa acaacaaaat gtttattatg taacctttaa
     1321 agttaaagga agaaaagtgg aagcagaagt gatagcttct ccttatgagt atattttgct
     1381 gtcgccaaca gatgttcctt ggttaacaca gcaaccactt cagttaacaa ttttagttcc
     1441 tcttcaagaa tatcaagaga aaatcttaag taagactgct cttccagaag atcaaaaaca
     1501 acaattaaaa accttgtttg tcaagtatga caatctatgg caacattggg aaaatcaagt
     1561 cgggcataga aaaattaggc cacataatat agcaactggt gattatcctc ctcgccctca
     1621 aaaacaatat cctattaatc ctaaggcaaa gcctagtata caaattgtaa tagatgactt
     1681 attgaaacaa ggggtgttaa cgcctcaaaa tagtacaatg aatacaccag tgtatcctgt
     1741 tcctaaacca gatggaaggt ggagaatggt attagattat agagaagtaa ataaaactat
     1801 tccattaaca gctgcccaaa accaacactc tgctggtatt ttagctacta ttgttagaca
     1861 aaaatataaa actaccttag atttagctaa tggattttgg gctcatccta ttacaccaga
     1921 atcttattgg ttaacagcat ttacctggca aggtaaacag tattgttgga cacgtcttcc
     1981 tcaaggattt ttaaatagtc cagcattgtt tacagctgat gtagtagatt tactaaaaga
     2041 aatccctaat gtacaagtgt atgttgatga tatatattta agccatgatg atcctaaaga
     2101 gcatgttcaa caattagaaa aagtgtttca aattttacta caggcaggat atgtagtatc
     2161 tttgaaaaaa tcagaaattg gtcaaaaaac tgtagaattt ttaggattta atattactaa
     2221 agaaggtcgt ggcctaacag acacttttaa aacaaaactg ttaaatatta ctcctccaaa
     2281 agacttaaag caattacaaa gcatattagg attgttaaat tttgctagaa attttatacc
     2341 taattttgct gaactggtac aaccattata caatttaata gcctcagcaa aaggcaaata
     2401 tattgagtgg tctgaagaaa atactaaaca attaaatatg gtaatagaag cattaaacac
     2461 tgcctctaat ttagaagaaa ggttaccaga acagagactg gtaattaaag tcaatacttc
     2521 tccatcagca ggatatgtaa gatattataa tgagactggt aaaaagccta ttatgtacct
     2581 aaattatgtg ttttccaaag cagaattaaa attttctatg ttagaaaaac tattaactac
     2641 aatgcacaaa gccttaatta aggctatgga tttggccatg ggacaagaaa tattagttta
     2701 tagtcccatt gtatctatga ctaaaataca aaaaactcca ctaccagaaa gaaaagcttt
     2761 acccattaga tggataacat ggatgactta tttagaagat ccaagaatcc aatttcatta
     2821 tgataaaacc ttaccagaac ttaagcatat tccagatgta tatacatcta gtcagtctcc
     2881 tgttaaacat ccttctcaat atgaaggagt gttttatact gatggctcgg ccatcaaaag
     2941 tcctgatcct acaaaaagca ataatgctgg catgggaata gtacatgcca catacaaacc
     3001 tgaatatcaa gttttgaatc aatggtcaat accactaggt aatcatactg ctcagatggc
     3061 tgaaatagct gcagttgaat ttgcctgtaa aaaagcttta aaaatacctg gtcctgtatt
     3121 agttataact gatagtttct atgtagcaga aagtgctaat aaagaattac catactggaa
     3181 atctaatggg tttgttaata ataagaaaaa gcctcttaaa catatctcca aatggaagtc
     3241 tattgctgag tgtttatcta tgaaaccaga cattactatt caacatgaaa aagggcatca
     3301 gcctacaaat accagtattc atactgaagg caatgcccta gcagataagc ttgccaccca
     3361 aggaagttat gtagttaatt gtaataccaa aaaaccaaac ctggatgcag agttggatca
     3421 attattacag ggtcattata taaaaggata tcccaaacaa tatacatatt ttttagaaga
     3481 tggcaaagta aaagtttcca gacctgaagg ggttaaaatt attccccctc agtcagacag
     3541 acaaaaaatt gtgcttcaag cccacaattt ggctcacacc ggacgtgaag ccactctttt
     3601 aaaaattgcc aacctttatt ggtggccaaa tatgagaaag gatgtggtta aacaactagg
     3661 acgctgtcaa cagtgtttaa tcacaaatgc ttccaacaaa gcctctggtc ctattctaag
     3721 accagatagg cctcaaaaac cttttgataa attctttatt gtctatattg gacctttgcc
     3781 accttcacag ggatacctat atgtattagt agttgttgat ggaatgacag gattcacttg
     3841 gttatacccc actaaggctc cttctactag cgcaactgtt aaatctctca atgtactcac
     3901 tagtattgca attccaaagg tgattcactc tgctcaaggt gcagcattca cttcttcaac
     3961 ctttgctgaa tgggcaaagg aaagaggtat acatttggaa ttcagtactc cttatcaccc
     4021 ccaaagtagt ggtaaggtgg aaaggaaaaa tagtgatata aaacgacttt taactaaact
     4081 gctagtagga agacccacaa agtggtatga cctattgcct gttgtacaac ttgctttaaa
     4141 caacacctat agccctgtat taaaatatac tccacatcaa ctcttatttg gtatagattc
     4201 aaatactcca tttgcaaatc aagatacact tgacttgacc agagaagaag aactttctct
     4261 tttacaggaa attcgtactt ctttatacca tccatccacc cctccaacct cctctcgttc
     4321 ctggtctcct gttgttggcc aattggtcca ggagagggtg gctaggcctg cttctttgag
     4381 acctcgttgg cataaaccgt ctactgtact taaggtgttg aatccaagga ctgttgttat
     4441 tttggaccat cttggcaaca acagaactgt aagtatagat aatttaaaac ctacttctca
     4501 tcagaatggc accaccaatg acactgcaac aatggatcat ttggaacaaa atgaataaag
     4561 cgcatgaggc acttcagaat acaacaactg tgactgaaca gcagaaggaa caaattatac
     4621 tggacattca aaatgaagaa gtacaaccaa ctaggagaga taaatttaga tatctgcttt
     4681 atacttgttg tgctactagc tcaagagtat tggcctggat atttttagtt tgtatattgt
     4741 taatcattgt tttggtttca tgctttgtga ctatatccag aatacaatgg aataaggata
     4801 ttcaggtatt aggacctgta atagactgga atgttactca aagagctgtt tatcaaccct
     4861 tacagactag aaggattgca cgttccctta gaatgcagca tcctgttcca aaatatgtgg
     4921 aggtaaatat gactagtatt ccacaaggtg tatactatga accccatccg gttgcggccg
     4981 cggggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     5041 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     5101 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     5161 ggaggtgtgg gaggtttttt cggatcctct agagtcgacc tgcaggcatg caagcttggc
     5221 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     5281 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5341 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5401 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5461 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5521 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5581 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5641 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5701 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5761 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5821 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5881 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5941 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6001 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6061 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6121 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6181 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6241 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6301 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6361 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6421 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6481 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6541 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6601 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6661 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6721 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6781 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6841 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6901 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6961 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7021 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7081 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7141 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7201 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7261 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7321 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7381 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     7441 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     7501 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     7561 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     7621 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7681 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     7741 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     7801 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagt
//
